The potential value of lncRNA-BC050642 in osteosarcoma origination and outcomes
Background The purpose of our research was to explore potential value of lncRNA-BC050642 in osteosarcoma origination and prognosis.Methods In this study, the tissue specimens were collected from 97 osteosarcoma patients and 97 age-matched healthy controls. Besides, human osteosarcoma cell lines U2OS...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1611593 |
| _version_ | 1817981472420659200 |
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| author | Yang Yang Mengxue Fei Xinying Zhou Yuejun Li Dadi Jin |
| author_facet | Yang Yang Mengxue Fei Xinying Zhou Yuejun Li Dadi Jin |
| author_sort | Yang Yang |
| collection | DOAJ |
| description | Background The purpose of our research was to explore potential value of lncRNA-BC050642 in osteosarcoma origination and prognosis.Methods In this study, the tissue specimens were collected from 97 osteosarcoma patients and 97 age-matched healthy controls. Besides, human osteosarcoma cell lines U2OS and normal osteoblastic cell line hFOB1.19 were selected for experiments in vitro. lncRNA-BC050642 levels were measured through quantitative real-time polymerase chain reaction (qRT-PCR). Relative expression of the gene c-myc was determined via ELISA analysis. x2 test was implemented to appraise possible relationship between BC050642 level and clinicopathological features. Cell proliferation assay, plate colony formation assay, and cell apoptosis assay were adopted to analyze the influence of BC050642 on tumor development. Besides, prognostic value of BC050642 was estimated using Kaplan–Meier and cox regression analysis.Results BC050642 levels showed distinctive increases in osteosarcoma tissues and cell lines compared with controls. And c-myc expression was down-regulated in osteosarcoma. There was a negative correlation between the expressions of BC050642 and c-myc. BC050642 expression was proved to be significantly correlated with Ennking and histological type. Up-regulated BC050642 could promote cell proliferation, induce colony formation and meanwhile inhibit cell apoptosis.Conclusions BC050642 is up-regulated in osteosarcma and its over-expression promotes tumor development via down-regulating the expression of c-myc. It also may be an independent prognostic biomarker for osteosarcoma. |
| first_indexed | 2024-04-13T23:06:11Z |
| format | Article |
| id | doaj.art-73515885b2d24effa8136b87204f4859 |
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| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-13T23:06:11Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
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| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-73515885b2d24effa8136b87204f48592022-12-22T02:25:41ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014711859186610.1080/21691401.2019.1611593The potential value of lncRNA-BC050642 in osteosarcoma origination and outcomesYang Yang0Mengxue Fei1Xinying Zhou2Yuejun Li3Dadi Jin4Department of Orthopedics, Academy of Orthopedics of Guangdong Province, the Third Affiliated Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Environmental Health, School of Public Health, Guangxi Medical University, Nanning, ChinaDepartment of Orthopedics, Academy of Orthopedics of Guangdong Province, the Third Affiliated Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Orthopedics, Academy of Orthopedics of Guangdong Province, the Third Affiliated Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Orthopedics, Academy of Orthopedics of Guangdong Province, the Third Affiliated Hospital, Southern Medical University, Guangzhou, ChinaBackground The purpose of our research was to explore potential value of lncRNA-BC050642 in osteosarcoma origination and prognosis.Methods In this study, the tissue specimens were collected from 97 osteosarcoma patients and 97 age-matched healthy controls. Besides, human osteosarcoma cell lines U2OS and normal osteoblastic cell line hFOB1.19 were selected for experiments in vitro. lncRNA-BC050642 levels were measured through quantitative real-time polymerase chain reaction (qRT-PCR). Relative expression of the gene c-myc was determined via ELISA analysis. x2 test was implemented to appraise possible relationship between BC050642 level and clinicopathological features. Cell proliferation assay, plate colony formation assay, and cell apoptosis assay were adopted to analyze the influence of BC050642 on tumor development. Besides, prognostic value of BC050642 was estimated using Kaplan–Meier and cox regression analysis.Results BC050642 levels showed distinctive increases in osteosarcoma tissues and cell lines compared with controls. And c-myc expression was down-regulated in osteosarcoma. There was a negative correlation between the expressions of BC050642 and c-myc. BC050642 expression was proved to be significantly correlated with Ennking and histological type. Up-regulated BC050642 could promote cell proliferation, induce colony formation and meanwhile inhibit cell apoptosis.Conclusions BC050642 is up-regulated in osteosarcma and its over-expression promotes tumor development via down-regulating the expression of c-myc. It also may be an independent prognostic biomarker for osteosarcoma.https://www.tandfonline.com/doi/10.1080/21691401.2019.1611593OsteosarcomaLncRNA-BC050642C-myctumor developmentprognosis |
| spellingShingle | Yang Yang Mengxue Fei Xinying Zhou Yuejun Li Dadi Jin The potential value of lncRNA-BC050642 in osteosarcoma origination and outcomes Artificial Cells, Nanomedicine, and Biotechnology Osteosarcoma LncRNA-BC050642 C-myc tumor development prognosis |
| title | The potential value of lncRNA-BC050642 in osteosarcoma origination and outcomes |
| title_full | The potential value of lncRNA-BC050642 in osteosarcoma origination and outcomes |
| title_fullStr | The potential value of lncRNA-BC050642 in osteosarcoma origination and outcomes |
| title_full_unstemmed | The potential value of lncRNA-BC050642 in osteosarcoma origination and outcomes |
| title_short | The potential value of lncRNA-BC050642 in osteosarcoma origination and outcomes |
| title_sort | potential value of lncrna bc050642 in osteosarcoma origination and outcomes |
| topic | Osteosarcoma LncRNA-BC050642 C-myc tumor development prognosis |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1611593 |
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