Composite polyelectrolyte multilayers for biofunctionalization of medical devices
Polyelectrolyte multilayer coatings (PEM) are prepared by alternative layer-by-layer deposition of cationic and anionic polyelectrolyte monolayers on charged surfaces. The thickness of the coatings ranges from nm to few μm. Their properties such as roughness, stiffness, surface charge and surface en...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2020-09-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2020-3110 |
| _version_ | 1818404209758830592 |
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| author | Rudt Alexander Andreeva Tonya D. Krastev Rumen Taneva Stefka G. |
| author_facet | Rudt Alexander Andreeva Tonya D. Krastev Rumen Taneva Stefka G. |
| author_sort | Rudt Alexander |
| collection | DOAJ |
| description | Polyelectrolyte multilayer coatings (PEM) are prepared by alternative layer-by-layer deposition of cationic and anionic polyelectrolyte monolayers on charged surfaces. The thickness of the coatings ranges from nm to few μm. Their properties such as roughness, stiffness, surface charge and surface energy can be precisely tuned to fulfill different technical or biological requirements. The coating process is based on self-assembly of polyelectrolytes. Advantages of these coatings are their easy handling, no harsh chemistry and the possibility for coatings on complex geometries. The PEM coatings can be prepared from a variety of suitable polyelectrolytes. Their stability varies from very durable PEM coatings that are only soluble in strong solvents to quickly degradable, which may be applied as drug release system. One example of such a degradable PEM system is the one based on the polyelectrolyte pair Hyaluronan (HA) and Chitosan (CHI). These biopolymers originate from natural sources and show low toxicity towards human cells. However, HA/CHI multilayers shows only weak adhesiveness for human umbilical vein endothelial cells (HUVEC). In this article, we summarize our approaches to enhance the HA/CHI multilayer by incorporation of a non-polymer substance -graphene oxide- to improve the cell adhesion and keep such properties as low cytotoxicity and biodegradability. Different approaches for incorporation of graphene oxide were performed and the cellular adhesion was tested by metabolic assay. |
| first_indexed | 2024-12-14T08:36:31Z |
| format | Article |
| id | doaj.art-7356671095c745fe88a55c6eb47861ad |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-12-14T08:36:31Z |
| publishDate | 2020-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-7356671095c745fe88a55c6eb47861ad2022-12-21T23:09:24ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042020-09-016342642910.1515/cdbme-2020-3110cdbme-2020-3110Composite polyelectrolyte multilayers for biofunctionalization of medical devicesRudt Alexander0Andreeva Tonya D.1Krastev Rumen2Taneva Stefka G.3Reutlingen University,Reutlingen, GermanyReutlingen University, Reutlingen, Germany and NMI Natural and Medical Sciences Institute at the University of Tübingen,Reutlingen, GermanyReutlingen University, Reutlingen, Germany and NMI Natural and Medical Sciences Institute at the University of Tübingen,Reutlingen, GermanyInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences,Sofia, BulgariaPolyelectrolyte multilayer coatings (PEM) are prepared by alternative layer-by-layer deposition of cationic and anionic polyelectrolyte monolayers on charged surfaces. The thickness of the coatings ranges from nm to few μm. Their properties such as roughness, stiffness, surface charge and surface energy can be precisely tuned to fulfill different technical or biological requirements. The coating process is based on self-assembly of polyelectrolytes. Advantages of these coatings are their easy handling, no harsh chemistry and the possibility for coatings on complex geometries. The PEM coatings can be prepared from a variety of suitable polyelectrolytes. Their stability varies from very durable PEM coatings that are only soluble in strong solvents to quickly degradable, which may be applied as drug release system. One example of such a degradable PEM system is the one based on the polyelectrolyte pair Hyaluronan (HA) and Chitosan (CHI). These biopolymers originate from natural sources and show low toxicity towards human cells. However, HA/CHI multilayers shows only weak adhesiveness for human umbilical vein endothelial cells (HUVEC). In this article, we summarize our approaches to enhance the HA/CHI multilayer by incorporation of a non-polymer substance -graphene oxide- to improve the cell adhesion and keep such properties as low cytotoxicity and biodegradability. Different approaches for incorporation of graphene oxide were performed and the cellular adhesion was tested by metabolic assay.https://doi.org/10.1515/cdbme-2020-3110polyelectrolyte multilayersbiocompatibilitycell adhesioncomposite filmsgraphene oxide |
| spellingShingle | Rudt Alexander Andreeva Tonya D. Krastev Rumen Taneva Stefka G. Composite polyelectrolyte multilayers for biofunctionalization of medical devices Current Directions in Biomedical Engineering polyelectrolyte multilayers biocompatibility cell adhesion composite films graphene oxide |
| title | Composite polyelectrolyte multilayers for biofunctionalization of medical devices |
| title_full | Composite polyelectrolyte multilayers for biofunctionalization of medical devices |
| title_fullStr | Composite polyelectrolyte multilayers for biofunctionalization of medical devices |
| title_full_unstemmed | Composite polyelectrolyte multilayers for biofunctionalization of medical devices |
| title_short | Composite polyelectrolyte multilayers for biofunctionalization of medical devices |
| title_sort | composite polyelectrolyte multilayers for biofunctionalization of medical devices |
| topic | polyelectrolyte multilayers biocompatibility cell adhesion composite films graphene oxide |
| url | https://doi.org/10.1515/cdbme-2020-3110 |
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