Study on antimicrobial activity of sturgeon skin mucus polypeptides (Rational Design, Self-Assembly and Application)
Despite the favorable biocompatibility of natural antimicrobial peptides (AMPs), their scarcity limits their practical application. Through rational design, the activity of AMPs can be enhanced to expand their application. In this study, we selected a natural sturgeon epidermal mucus peptide, AP-16...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524001238 |
| _version_ | 1797244843550834688 |
|---|---|
| author | Beining Yang Wei Li Yuxuan Mao Yuanhui Zhao Yong Xue Xinxing Xu Yilin Zhao Kang Liu |
| author_facet | Beining Yang Wei Li Yuxuan Mao Yuanhui Zhao Yong Xue Xinxing Xu Yilin Zhao Kang Liu |
| author_sort | Beining Yang |
| collection | DOAJ |
| description | Despite the favorable biocompatibility of natural antimicrobial peptides (AMPs), their scarcity limits their practical application. Through rational design, the activity of AMPs can be enhanced to expand their application. In this study, we selected a natural sturgeon epidermal mucus peptide, AP-16 (APATPAAPALLPLWLL), as the model molecule and studied its conformational regulation and antimicrobial activity through amino acid substitutions and N-terminal lipidation. The structural and morphological transitions of the peptide self-assemblies were investigated using circular dichroism and transmission electron microscopy. Following amino acid substitution, the conformation of AL-16 (AKATKAAKALLKLWLL) did not change. Following N-terminal alkylation, the C8-AL-16 and C12-AL-16 conformations changed from random coil to β-sheet or α-helix, and the self-assembly changed from nanofibers to nanospheres. AL-16, C8-AL-16, and C8-AL-16 presented significant antimicrobial activity against Pseudomonas and Shewanella at low concentrations. N-terminal alkylation effectively extended the shelf life of Litopenaeus vannamei. These results support the application of natural AMPs. |
| first_indexed | 2024-04-24T19:17:27Z |
| format | Article |
| id | doaj.art-7357dc05a5e044cca464287d565cce36 |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-04-24T19:17:27Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-7357dc05a5e044cca464287d565cce362024-03-26T04:27:43ZengElsevierFood Chemistry: X2590-15752024-03-0121101236Study on antimicrobial activity of sturgeon skin mucus polypeptides (Rational Design, Self-Assembly and Application)Beining Yang0Wei Li1Yuxuan Mao2Yuanhui Zhao3Yong Xue4Xinxing Xu5Yilin Zhao6Kang Liu7Sanya Oceanographic Institution /College of Food Science and Engineering, Ocean University of China, Sanya/Qingdao, China; State Key Laboratory of Marine Food Processing & Safety Control, Ocean University of China, Qingdao, Shandong, ChinaChina Department of General Surgery, The District Hospital of Qingdao West Coast New Area, Qngdao, Shandong, ChinaNational Engineering Research Center for Wheat and Corn Deep Processing, College of Food Science and Engineering, Jilin Agricultural University, Changchun, Jilin, ChinaSanya Oceanographic Institution /College of Food Science and Engineering, Ocean University of China, Sanya/Qingdao, China; State Key Laboratory of Marine Food Processing & Safety Control, Ocean University of China, Qingdao, Shandong, ChinaSanya Oceanographic Institution /College of Food Science and Engineering, Ocean University of China, Sanya/Qingdao, China; State Key Laboratory of Marine Food Processing & Safety Control, Ocean University of China, Qingdao, Shandong, ChinaState Key Laboratory of Marine Food Processing & Safety Control, Ocean University of China, Qingdao, Shandong, ChinaSanya Oceanographic Institution /College of Food Science and Engineering, Ocean University of China, Sanya/Qingdao, China; State Key Laboratory of Marine Food Processing & Safety Control, Ocean University of China, Qingdao, Shandong, China; Corresponding authors at: Sanya Oceanographic Institution /College of Food Science and Engineering/State Key Laboratory of Marine Food Processing & Safety Control, Ocean University of China, Qingdao, Shandong, China (Y. Zhao).College of Ocean Food and Biological Engineering, Jimei University, Xiamen, China; National & Local Joint Engineering Research Center of Processing Technology for Aquatic Products, Xiamen, China; Corresponding authors at: Sanya Oceanographic Institution /College of Food Science and Engineering/State Key Laboratory of Marine Food Processing & Safety Control, Ocean University of China, Qingdao, Shandong, China (Y. Zhao).Despite the favorable biocompatibility of natural antimicrobial peptides (AMPs), their scarcity limits their practical application. Through rational design, the activity of AMPs can be enhanced to expand their application. In this study, we selected a natural sturgeon epidermal mucus peptide, AP-16 (APATPAAPALLPLWLL), as the model molecule and studied its conformational regulation and antimicrobial activity through amino acid substitutions and N-terminal lipidation. The structural and morphological transitions of the peptide self-assemblies were investigated using circular dichroism and transmission electron microscopy. Following amino acid substitution, the conformation of AL-16 (AKATKAAKALLKLWLL) did not change. Following N-terminal alkylation, the C8-AL-16 and C12-AL-16 conformations changed from random coil to β-sheet or α-helix, and the self-assembly changed from nanofibers to nanospheres. AL-16, C8-AL-16, and C8-AL-16 presented significant antimicrobial activity against Pseudomonas and Shewanella at low concentrations. N-terminal alkylation effectively extended the shelf life of Litopenaeus vannamei. These results support the application of natural AMPs.http://www.sciencedirect.com/science/article/pii/S2590157524001238Sturgeon epidermal mucusAntimicrobial peptideSelf-assemblyMolecular designAntimicrobial activity |
| spellingShingle | Beining Yang Wei Li Yuxuan Mao Yuanhui Zhao Yong Xue Xinxing Xu Yilin Zhao Kang Liu Study on antimicrobial activity of sturgeon skin mucus polypeptides (Rational Design, Self-Assembly and Application) Food Chemistry: X Sturgeon epidermal mucus Antimicrobial peptide Self-assembly Molecular design Antimicrobial activity |
| title | Study on antimicrobial activity of sturgeon skin mucus polypeptides (Rational Design, Self-Assembly and Application) |
| title_full | Study on antimicrobial activity of sturgeon skin mucus polypeptides (Rational Design, Self-Assembly and Application) |
| title_fullStr | Study on antimicrobial activity of sturgeon skin mucus polypeptides (Rational Design, Self-Assembly and Application) |
| title_full_unstemmed | Study on antimicrobial activity of sturgeon skin mucus polypeptides (Rational Design, Self-Assembly and Application) |
| title_short | Study on antimicrobial activity of sturgeon skin mucus polypeptides (Rational Design, Self-Assembly and Application) |
| title_sort | study on antimicrobial activity of sturgeon skin mucus polypeptides rational design self assembly and application |
| topic | Sturgeon epidermal mucus Antimicrobial peptide Self-assembly Molecular design Antimicrobial activity |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524001238 |
| work_keys_str_mv | AT beiningyang studyonantimicrobialactivityofsturgeonskinmucuspolypeptidesrationaldesignselfassemblyandapplication AT weili studyonantimicrobialactivityofsturgeonskinmucuspolypeptidesrationaldesignselfassemblyandapplication AT yuxuanmao studyonantimicrobialactivityofsturgeonskinmucuspolypeptidesrationaldesignselfassemblyandapplication AT yuanhuizhao studyonantimicrobialactivityofsturgeonskinmucuspolypeptidesrationaldesignselfassemblyandapplication AT yongxue studyonantimicrobialactivityofsturgeonskinmucuspolypeptidesrationaldesignselfassemblyandapplication AT xinxingxu studyonantimicrobialactivityofsturgeonskinmucuspolypeptidesrationaldesignselfassemblyandapplication AT yilinzhao studyonantimicrobialactivityofsturgeonskinmucuspolypeptidesrationaldesignselfassemblyandapplication AT kangliu studyonantimicrobialactivityofsturgeonskinmucuspolypeptidesrationaldesignselfassemblyandapplication |