Microbiota continuum along the chicken oviduct and its association with host genetics and egg formation
ABSTRACT: The microbiota of female reproductive tract have attracted considerable attention in recent years due to their effects on host fitness. However, the microbiota throughout the chicken oviduct and its symbiotic relationships with the host have not been well characterized. Here, we characteri...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-07-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579121001383 |
| _version_ | 1818724336293380096 |
|---|---|
| author | Chaoliang Wen Quanlin Li Fangren Lan Xiaochang Li Guangqi Li Yiyuan Yan Guiqin Wu Ning Yang Congjiao Sun |
| author_facet | Chaoliang Wen Quanlin Li Fangren Lan Xiaochang Li Guangqi Li Yiyuan Yan Guiqin Wu Ning Yang Congjiao Sun |
| author_sort | Chaoliang Wen |
| collection | DOAJ |
| description | ABSTRACT: The microbiota of female reproductive tract have attracted considerable attention in recent years due to their effects on host fitness. However, the microbiota throughout the chicken oviduct and its symbiotic relationships with the host have not been well characterized. Here, we characterized the microbial composition of six segments of the reproductive tract, including the infundibulum, magnum, isthmus, uterus, vagina and cloaca, in pedigreed laying hens with phenotypes of egg quality and quantity. We found that the microbial diversity gradually increased along the reproductive tract from the infundibulum to the cloaca, and the microbial communities were distinct among the cloaca, vagina and four other oviductal segments. The magnum exhibited the lowest diversity, given that the lysozyme and other antimicrobial proteins are secreted at this location. The results of correlation estimated showed that the relationship between host genetic kinship and microbial distance was negligible. Additionally, the genetically related pairwise individuals did not exhibit a more similar microbial community than unrelated pairs. Although the egg might be directly contaminated with potential pathogenic bacteria during egg formation and oviposition, some microorganisms provide long-term benefits to the host. Among these, we observed that increased abundance of vaginal Staphylococcus and Ralstonia was significantly associated with darker eggshells. Meanwhile, vaginal Romboutsia could be used as a predictor for egg number. These findings provide insight into the nature of the chicken reproductive tract microbiota and highlight the effect of oviductal bacteria on the process of egg formation. |
| first_indexed | 2024-12-17T21:24:48Z |
| format | Article |
| id | doaj.art-73599cc5224649369fca116511cb3281 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-17T21:24:48Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-73599cc5224649369fca116511cb32812022-12-21T21:32:03ZengElsevierPoultry Science0032-57912021-07-011007101104Microbiota continuum along the chicken oviduct and its association with host genetics and egg formationChaoliang Wen0Quanlin Li1Fangren Lan2Xiaochang Li3Guangqi Li4Yiyuan Yan5Guiqin Wu6Ning Yang7Congjiao Sun8National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaBeijing Engineering Research Centre of Layer, Beijing 101206, ChinaBeijing Engineering Research Centre of Layer, Beijing 101206, ChinaBeijing Engineering Research Centre of Layer, Beijing 101206, ChinaNational Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, China; Corresponding author:ABSTRACT: The microbiota of female reproductive tract have attracted considerable attention in recent years due to their effects on host fitness. However, the microbiota throughout the chicken oviduct and its symbiotic relationships with the host have not been well characterized. Here, we characterized the microbial composition of six segments of the reproductive tract, including the infundibulum, magnum, isthmus, uterus, vagina and cloaca, in pedigreed laying hens with phenotypes of egg quality and quantity. We found that the microbial diversity gradually increased along the reproductive tract from the infundibulum to the cloaca, and the microbial communities were distinct among the cloaca, vagina and four other oviductal segments. The magnum exhibited the lowest diversity, given that the lysozyme and other antimicrobial proteins are secreted at this location. The results of correlation estimated showed that the relationship between host genetic kinship and microbial distance was negligible. Additionally, the genetically related pairwise individuals did not exhibit a more similar microbial community than unrelated pairs. Although the egg might be directly contaminated with potential pathogenic bacteria during egg formation and oviposition, some microorganisms provide long-term benefits to the host. Among these, we observed that increased abundance of vaginal Staphylococcus and Ralstonia was significantly associated with darker eggshells. Meanwhile, vaginal Romboutsia could be used as a predictor for egg number. These findings provide insight into the nature of the chicken reproductive tract microbiota and highlight the effect of oviductal bacteria on the process of egg formation.http://www.sciencedirect.com/science/article/pii/S0032579121001383chicken reproductive tractmicrobiotahost geneticsmicrobial contaminationegg quality |
| spellingShingle | Chaoliang Wen Quanlin Li Fangren Lan Xiaochang Li Guangqi Li Yiyuan Yan Guiqin Wu Ning Yang Congjiao Sun Microbiota continuum along the chicken oviduct and its association with host genetics and egg formation Poultry Science chicken reproductive tract microbiota host genetics microbial contamination egg quality |
| title | Microbiota continuum along the chicken oviduct and its association with host genetics and egg formation |
| title_full | Microbiota continuum along the chicken oviduct and its association with host genetics and egg formation |
| title_fullStr | Microbiota continuum along the chicken oviduct and its association with host genetics and egg formation |
| title_full_unstemmed | Microbiota continuum along the chicken oviduct and its association with host genetics and egg formation |
| title_short | Microbiota continuum along the chicken oviduct and its association with host genetics and egg formation |
| title_sort | microbiota continuum along the chicken oviduct and its association with host genetics and egg formation |
| topic | chicken reproductive tract microbiota host genetics microbial contamination egg quality |
| url | http://www.sciencedirect.com/science/article/pii/S0032579121001383 |
| work_keys_str_mv | AT chaoliangwen microbiotacontinuumalongthechickenoviductanditsassociationwithhostgeneticsandeggformation AT quanlinli microbiotacontinuumalongthechickenoviductanditsassociationwithhostgeneticsandeggformation AT fangrenlan microbiotacontinuumalongthechickenoviductanditsassociationwithhostgeneticsandeggformation AT xiaochangli microbiotacontinuumalongthechickenoviductanditsassociationwithhostgeneticsandeggformation AT guangqili microbiotacontinuumalongthechickenoviductanditsassociationwithhostgeneticsandeggformation AT yiyuanyan microbiotacontinuumalongthechickenoviductanditsassociationwithhostgeneticsandeggformation AT guiqinwu microbiotacontinuumalongthechickenoviductanditsassociationwithhostgeneticsandeggformation AT ningyang microbiotacontinuumalongthechickenoviductanditsassociationwithhostgeneticsandeggformation AT congjiaosun microbiotacontinuumalongthechickenoviductanditsassociationwithhostgeneticsandeggformation |