Development of polyclonal antibodies-based serological methods for detection of the rehmannia mosaic virus in field plants
Rehmannia glutinosa is a top-grade traditional Chinese medicine, and also is an important planting medicinal material for Chinese poor farmers shaking off poverty. Rehmannia mosaic virus (ReMV) causes big economic loss of R. glutinosa in planting area. However, there is no effective methods for quic...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsufs.2022.1013470/full |
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| author | Jiahuan Chen Chenwei Feng Xiao Guo Yuchen Zhou Tianxiao Gu Xinjian Zhuang Lei Cheng Kun Zhang Kun Zhang |
| author_facet | Jiahuan Chen Chenwei Feng Xiao Guo Yuchen Zhou Tianxiao Gu Xinjian Zhuang Lei Cheng Kun Zhang Kun Zhang |
| author_sort | Jiahuan Chen |
| collection | DOAJ |
| description | Rehmannia glutinosa is a top-grade traditional Chinese medicine, and also is an important planting medicinal material for Chinese poor farmers shaking off poverty. Rehmannia mosaic virus (ReMV) causes big economic loss of R. glutinosa in planting area. However, there is no effective methods for quick, accurate, and high-throughput detection for ReMV in Chinese production area. The preserved R. glutinosa samples carrying ReMV was taken for research material. The coat protein coding sequences (CPReMV) was cloned and sequenced. The target sequence was further placed into a prokaryotic expression vector to express the N-terminal-tagged recombinant CPReMV protein (His-CPReMV). Purified His-CPReMV was used as an antigen to immunize New Zealand white rabbits, and antiserum was obtained. The titers and sensitivities of the antisera were analyzed and evaluated. Polyclonal antibodies were purified from the antiserum, and the titers and sensitivity to the target His-CPReMV protein were evaluated. The results demonstrate that the obtained polyclonal antibodies against His-CPReMV could be successfully used for rapid, accurate, and high-throughput detection of ReMV from R. glutinosa planted in the wild. Our investigation established serological-based detection methods for ReMV for the first time, and provides a foundation for future exploration of the pathogenic mechanisms of ReMV in R. glutinosa. |
| first_indexed | 2024-04-11T10:16:14Z |
| format | Article |
| id | doaj.art-7359a35c7d624a79817a9d55d9e610fc |
| institution | Directory Open Access Journal |
| issn | 2571-581X |
| language | English |
| last_indexed | 2024-04-11T10:16:14Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sustainable Food Systems |
| spelling | doaj.art-7359a35c7d624a79817a9d55d9e610fc2022-12-22T04:29:56ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2022-10-01610.3389/fsufs.2022.10134701013470Development of polyclonal antibodies-based serological methods for detection of the rehmannia mosaic virus in field plantsJiahuan Chen0Chenwei Feng1Xiao Guo2Yuchen Zhou3Tianxiao Gu4Xinjian Zhuang5Lei Cheng6Kun Zhang7Kun Zhang8Department of Pharmacy, Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, ChinaDepartment of Plant Pathology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, ChinaDepartment of Plant Pathology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, ChinaDepartment of Pharmacy, Medical College of Yangzhou University, Yangzhou University, Yangzhou, ChinaDepartment of Plant Pathology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, ChinaDepartment of Plant Pathology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, ChinaBozhou Vocational and Technical College, Bozhou, ChinaDepartment of Plant Pathology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University, Nanjing, ChinaRehmannia glutinosa is a top-grade traditional Chinese medicine, and also is an important planting medicinal material for Chinese poor farmers shaking off poverty. Rehmannia mosaic virus (ReMV) causes big economic loss of R. glutinosa in planting area. However, there is no effective methods for quick, accurate, and high-throughput detection for ReMV in Chinese production area. The preserved R. glutinosa samples carrying ReMV was taken for research material. The coat protein coding sequences (CPReMV) was cloned and sequenced. The target sequence was further placed into a prokaryotic expression vector to express the N-terminal-tagged recombinant CPReMV protein (His-CPReMV). Purified His-CPReMV was used as an antigen to immunize New Zealand white rabbits, and antiserum was obtained. The titers and sensitivities of the antisera were analyzed and evaluated. Polyclonal antibodies were purified from the antiserum, and the titers and sensitivity to the target His-CPReMV protein were evaluated. The results demonstrate that the obtained polyclonal antibodies against His-CPReMV could be successfully used for rapid, accurate, and high-throughput detection of ReMV from R. glutinosa planted in the wild. Our investigation established serological-based detection methods for ReMV for the first time, and provides a foundation for future exploration of the pathogenic mechanisms of ReMV in R. glutinosa.https://www.frontiersin.org/articles/10.3389/fsufs.2022.1013470/fullrehmannia mosaic virusantiserumpolyclonal antibodiesdetectionapplication |
| spellingShingle | Jiahuan Chen Chenwei Feng Xiao Guo Yuchen Zhou Tianxiao Gu Xinjian Zhuang Lei Cheng Kun Zhang Kun Zhang Development of polyclonal antibodies-based serological methods for detection of the rehmannia mosaic virus in field plants Frontiers in Sustainable Food Systems rehmannia mosaic virus antiserum polyclonal antibodies detection application |
| title | Development of polyclonal antibodies-based serological methods for detection of the rehmannia mosaic virus in field plants |
| title_full | Development of polyclonal antibodies-based serological methods for detection of the rehmannia mosaic virus in field plants |
| title_fullStr | Development of polyclonal antibodies-based serological methods for detection of the rehmannia mosaic virus in field plants |
| title_full_unstemmed | Development of polyclonal antibodies-based serological methods for detection of the rehmannia mosaic virus in field plants |
| title_short | Development of polyclonal antibodies-based serological methods for detection of the rehmannia mosaic virus in field plants |
| title_sort | development of polyclonal antibodies based serological methods for detection of the rehmannia mosaic virus in field plants |
| topic | rehmannia mosaic virus antiserum polyclonal antibodies detection application |
| url | https://www.frontiersin.org/articles/10.3389/fsufs.2022.1013470/full |
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