Blood pressure and sugar regulating potentials of Anarcadium occidentale nut globulin and albumin hydrolysates
Several novel functional peptides have been successfully extracted from plant storage proteins. This study investigated the degree of hydrolysis, peptide yield, amino acid constituents, angiotensin converting enzyme (ACE), alpha amylase inhibitory and in vitro antioxidant activities of cashew (Anarc...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844021004898 |
| _version_ | 1818667944600666112 |
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| author | Rotimi Olusanya Arise Oluwaseun Oluwatosin Taofeek Kehinde Babaita Raphael Idowu Adeoye Omorefosa Osemwegie |
| author_facet | Rotimi Olusanya Arise Oluwaseun Oluwatosin Taofeek Kehinde Babaita Raphael Idowu Adeoye Omorefosa Osemwegie |
| author_sort | Rotimi Olusanya Arise |
| collection | DOAJ |
| description | Several novel functional peptides have been successfully extracted from plant storage proteins. This study investigated the degree of hydrolysis, peptide yield, amino acid constituents, angiotensin converting enzyme (ACE), alpha amylase inhibitory and in vitro antioxidant activities of cashew (Anarcardium occidentale) nut proteins (CNP) hydrolysates (CNPHs). Cashew nut proteins (albumin and globulin) were hydrolysed using pancreatin, Alcalase and trypsin. The peptide yield and degree of hydrolysis (DH) of CNP by pancreatin (75.69 ± 0.84%; 37.39 ± 0.31) was significantly higher than those by Alcalase (61.67 ± 0.55%; 23.87 ± 0.23) and trypsin (43.33 ± 0.45%; 11 ± 0.15). The inhibition of ACE by albumin and globulin hydrolysates was concentration dependent. At 1.2 mg/mL, ACE-inhibitory activity of pancreatic cashew nut globulin (CNGH) hydrolysate (51.65 ± 1.2%) was significantly higher than those of Alcalase (34.603 ± 0.65%) and tryptic (29.92 ± 0.73%) CNGHs. Cashew nut albumin hydrolysate (CNAH) demonstrated concentration-dependent alpha-amylase inhibition (IC50 0.17 ± 0.02–0.41 ± 0.021 mg/mL). The order of inhibition was tryptic > Alcalase > pancreatic CNAHs. The pancreatic hydrolysates of both albumin and globulin fractions displayed the highest DPPH antioxidant activity, while pancreatic CNAH was the most potent superoxide anion scavenger. These findings therefore posit that cashew nut globulin and albumin hydrolysates are laden with useful bioactive peptides that may be further explored for regulation of blood pressure and sugar in hypertensive and diabetic in vivo models. |
| first_indexed | 2024-12-17T06:28:28Z |
| format | Article |
| id | doaj.art-7359aa9f577e40b181af9e9c1464473f |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-17T06:28:28Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-7359aa9f577e40b181af9e9c1464473f2022-12-21T22:00:13ZengElsevierHeliyon2405-84402021-03-0173e06384Blood pressure and sugar regulating potentials of Anarcadium occidentale nut globulin and albumin hydrolysatesRotimi Olusanya Arise0Oluwaseun Oluwatosin Taofeek1Kehinde Babaita2Raphael Idowu Adeoye3Omorefosa Osemwegie4Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, P.M.B. 1515, Ilorin, Nigeria; Corresponding author.Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, P.M.B. 1515, Ilorin, NigeriaDepartment of Biochemistry, Faculty of Life Sciences, University of Ilorin, P.M.B. 1515, Ilorin, NigeriaDepartment of Biochemistry, Faculty of Life Sciences, University of Ilorin, P.M.B. 1515, Ilorin, NigeriaDepartment of Biological Sciences, College of Science and Engineering, Landmark University, Omu-Aran, NigeriaSeveral novel functional peptides have been successfully extracted from plant storage proteins. This study investigated the degree of hydrolysis, peptide yield, amino acid constituents, angiotensin converting enzyme (ACE), alpha amylase inhibitory and in vitro antioxidant activities of cashew (Anarcardium occidentale) nut proteins (CNP) hydrolysates (CNPHs). Cashew nut proteins (albumin and globulin) were hydrolysed using pancreatin, Alcalase and trypsin. The peptide yield and degree of hydrolysis (DH) of CNP by pancreatin (75.69 ± 0.84%; 37.39 ± 0.31) was significantly higher than those by Alcalase (61.67 ± 0.55%; 23.87 ± 0.23) and trypsin (43.33 ± 0.45%; 11 ± 0.15). The inhibition of ACE by albumin and globulin hydrolysates was concentration dependent. At 1.2 mg/mL, ACE-inhibitory activity of pancreatic cashew nut globulin (CNGH) hydrolysate (51.65 ± 1.2%) was significantly higher than those of Alcalase (34.603 ± 0.65%) and tryptic (29.92 ± 0.73%) CNGHs. Cashew nut albumin hydrolysate (CNAH) demonstrated concentration-dependent alpha-amylase inhibition (IC50 0.17 ± 0.02–0.41 ± 0.021 mg/mL). The order of inhibition was tryptic > Alcalase > pancreatic CNAHs. The pancreatic hydrolysates of both albumin and globulin fractions displayed the highest DPPH antioxidant activity, while pancreatic CNAH was the most potent superoxide anion scavenger. These findings therefore posit that cashew nut globulin and albumin hydrolysates are laden with useful bioactive peptides that may be further explored for regulation of blood pressure and sugar in hypertensive and diabetic in vivo models.http://www.sciencedirect.com/science/article/pii/S2405844021004898Cashew nut proteinAntioxidantsGlobulin and albumin hydrolysatesACE and Alpha amylase inhibition |
| spellingShingle | Rotimi Olusanya Arise Oluwaseun Oluwatosin Taofeek Kehinde Babaita Raphael Idowu Adeoye Omorefosa Osemwegie Blood pressure and sugar regulating potentials of Anarcadium occidentale nut globulin and albumin hydrolysates Heliyon Cashew nut protein Antioxidants Globulin and albumin hydrolysates ACE and Alpha amylase inhibition |
| title | Blood pressure and sugar regulating potentials of Anarcadium occidentale nut globulin and albumin hydrolysates |
| title_full | Blood pressure and sugar regulating potentials of Anarcadium occidentale nut globulin and albumin hydrolysates |
| title_fullStr | Blood pressure and sugar regulating potentials of Anarcadium occidentale nut globulin and albumin hydrolysates |
| title_full_unstemmed | Blood pressure and sugar regulating potentials of Anarcadium occidentale nut globulin and albumin hydrolysates |
| title_short | Blood pressure and sugar regulating potentials of Anarcadium occidentale nut globulin and albumin hydrolysates |
| title_sort | blood pressure and sugar regulating potentials of anarcadium occidentale nut globulin and albumin hydrolysates |
| topic | Cashew nut protein Antioxidants Globulin and albumin hydrolysates ACE and Alpha amylase inhibition |
| url | http://www.sciencedirect.com/science/article/pii/S2405844021004898 |
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