Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/11/728 |
| _version_ | 1797509374932942848 |
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| author | Xiaowei Song Jia Li Mohammad Mofidfar Richard N. Zare |
| author_facet | Xiaowei Song Jia Li Mohammad Mofidfar Richard N. Zare |
| author_sort | Xiaowei Song |
| collection | DOAJ |
| description | Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of the power of this approach, isobaric ions in urinary metabolic profiles are identified and used to distinguish between healthy individuals and those having bladder cancer. |
| first_indexed | 2024-03-10T05:17:49Z |
| format | Article |
| id | doaj.art-735dd03740714895acb0bd1e5a5ec44f |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T05:17:49Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-735dd03740714895acb0bd1e5a5ec44f2023-11-23T00:20:39ZengMDPI AGMetabolites2218-19892021-10-01111172810.3390/metabo11110728Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass SpectrometryXiaowei Song0Jia Li1Mohammad Mofidfar2Richard N. Zare3Department of Chemistry, Stanford University, Stanford, CA 94305, USADepartment of Chemistry, Fudan University, Shanghai 200438, ChinaDepartment of Chemistry, Stanford University, Stanford, CA 94305, USADepartment of Chemistry, Stanford University, Stanford, CA 94305, USAIsobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of the power of this approach, isobaric ions in urinary metabolic profiles are identified and used to distinguish between healthy individuals and those having bladder cancer.https://www.mdpi.com/2218-1989/11/11/728hydrogen-deuterium exchangeisobaric ionsambient ionization mass spectrometrymicrodroplets |
| spellingShingle | Xiaowei Song Jia Li Mohammad Mofidfar Richard N. Zare Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry Metabolites hydrogen-deuterium exchange isobaric ions ambient ionization mass spectrometry microdroplets |
| title | Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry |
| title_full | Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry |
| title_fullStr | Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry |
| title_full_unstemmed | Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry |
| title_short | Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry |
| title_sort | distinguishing between isobaric ions using microdroplet hydrogen deuterium exchange mass spectrometry |
| topic | hydrogen-deuterium exchange isobaric ions ambient ionization mass spectrometry microdroplets |
| url | https://www.mdpi.com/2218-1989/11/11/728 |
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