Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature?
Aim: To evaluate whether semen processing at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature (RT) by swim-up method. Settings: This study was done at tertiary care center attached to Reproductive Medicine Unit and Medical College. Design: Pros...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2013-01-01
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| Series: | Journal of Human Reproductive Sciences |
| Subjects: | |
| Online Access: | http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2013;volume=6;issue=1;spage=23;epage=26;aulast=Repalle |
| Summary: | Aim: To evaluate whether semen processing at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature (RT) by swim-up method. Settings: This study was done at tertiary care center attached to Reproductive Medicine Unit and Medical College. Design: Prospective pilot study. Patients: Normozoospermic men (n = 50) undergoing diagnostic semen analysis. Materials and Methods: Normozoospermic samples (World Health Organization, 2010 criteria) after analysis was divided into two aliquots (0.5 mL each); one was processed at 37°C and the other at RT by swim-up method. DNA fragmentation of both samples post wash was calculated by acridine orange method. Statistical Analysis Used: The values of sperm DNA fragmentation were represented as mean and standard error (mean ± SEM) of the mean. Paired t-test was used for calculating the sperm DNA integrity difference between post wash at RT and 37°C. Results: Statistically significant difference was not observed in post wash sperm DNA fragmentation values at 37°C compared to RT. Conclusion: Our data represents that there was no significant difference in sperm DNA fragmentation values of samples processed at 37°C and at RT. Hence, sperm processing at 37°C does not yield sperm with better DNA integrity compared to centrifugation and processing at RT. |
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| ISSN: | 0974-1208 1998-4766 |