Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature?
Aim: To evaluate whether semen processing at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature (RT) by swim-up method. Settings: This study was done at tertiary care center attached to Reproductive Medicine Unit and Medical College. Design: Pros...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2013-01-01
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| Series: | Journal of Human Reproductive Sciences |
| Subjects: | |
| Online Access: | http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2013;volume=6;issue=1;spage=23;epage=26;aulast=Repalle |
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| author | Deepthi Repalle Priya Bhave Chittawar Shilpa Bhandari Geetanjali Joshi Mansi Paranjape Charudutta Joshi |
| author_facet | Deepthi Repalle Priya Bhave Chittawar Shilpa Bhandari Geetanjali Joshi Mansi Paranjape Charudutta Joshi |
| author_sort | Deepthi Repalle |
| collection | DOAJ |
| description | Aim: To evaluate whether semen processing at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature (RT) by swim-up method. Settings: This study was done at tertiary care center attached to Reproductive Medicine Unit and Medical College. Design: Prospective pilot study. Patients: Normozoospermic men (n = 50) undergoing diagnostic semen analysis. Materials and Methods: Normozoospermic samples (World Health Organization, 2010 criteria) after analysis was divided into two aliquots (0.5 mL each); one was processed at 37°C and the other at RT by swim-up method. DNA fragmentation of both samples post wash was calculated by acridine orange method. Statistical Analysis Used: The values of sperm DNA fragmentation were represented as mean and standard error (mean ± SEM) of the mean. Paired t-test was used for calculating the sperm DNA integrity difference between post wash at RT and 37°C. Results: Statistically significant difference was not observed in post wash sperm DNA fragmentation values at 37°C compared to RT. Conclusion: Our data represents that there was no significant difference in sperm DNA fragmentation values of samples processed at 37°C and at RT. Hence, sperm processing at 37°C does not yield sperm with better DNA integrity compared to centrifugation and processing at RT. |
| first_indexed | 2024-12-14T21:24:26Z |
| format | Article |
| id | doaj.art-735f3c360eea48b998769cef01128bb6 |
| institution | Directory Open Access Journal |
| issn | 0974-1208 1998-4766 |
| language | English |
| last_indexed | 2024-12-14T21:24:26Z |
| publishDate | 2013-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Human Reproductive Sciences |
| spelling | doaj.art-735f3c360eea48b998769cef01128bb62022-12-21T22:46:52ZengWolters Kluwer Medknow PublicationsJournal of Human Reproductive Sciences0974-12081998-47662013-01-0161232610.4103/0974-1208.112375Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature?Deepthi RepallePriya Bhave ChittawarShilpa BhandariGeetanjali JoshiMansi ParanjapeCharudutta JoshiAim: To evaluate whether semen processing at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature (RT) by swim-up method. Settings: This study was done at tertiary care center attached to Reproductive Medicine Unit and Medical College. Design: Prospective pilot study. Patients: Normozoospermic men (n = 50) undergoing diagnostic semen analysis. Materials and Methods: Normozoospermic samples (World Health Organization, 2010 criteria) after analysis was divided into two aliquots (0.5 mL each); one was processed at 37°C and the other at RT by swim-up method. DNA fragmentation of both samples post wash was calculated by acridine orange method. Statistical Analysis Used: The values of sperm DNA fragmentation were represented as mean and standard error (mean ± SEM) of the mean. Paired t-test was used for calculating the sperm DNA integrity difference between post wash at RT and 37°C. Results: Statistically significant difference was not observed in post wash sperm DNA fragmentation values at 37°C compared to RT. Conclusion: Our data represents that there was no significant difference in sperm DNA fragmentation values of samples processed at 37°C and at RT. Hence, sperm processing at 37°C does not yield sperm with better DNA integrity compared to centrifugation and processing at RT.http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2013;volume=6;issue=1;spage=23;epage=26;aulast=RepalleAcridine orangesemen preparationsperm DNA integritytemperature |
| spellingShingle | Deepthi Repalle Priya Bhave Chittawar Shilpa Bhandari Geetanjali Joshi Mansi Paranjape Charudutta Joshi Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature? Journal of Human Reproductive Sciences Acridine orange semen preparation sperm DNA integrity temperature |
| title | Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature? |
| title_full | Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature? |
| title_fullStr | Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature? |
| title_full_unstemmed | Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature? |
| title_short | Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature? |
| title_sort | does centrifugation and semen processing with swim up at 37°c yield sperm with better dna integrity compared to centrifugation and processing at room temperature |
| topic | Acridine orange semen preparation sperm DNA integrity temperature |
| url | http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2013;volume=6;issue=1;spage=23;epage=26;aulast=Repalle |
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