Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes
Entamoeba histolytica produces Monocyte Locomotion Inhibitory Factor (MLIF), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. Leukocytes are affected through the modulation of cytokine expression and/or production. We evaluated the effects of MLIF on the activa...
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| Format: | Article |
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BMC
2009-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400003 |
| _version_ | 1818521465043025920 |
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| author | SARA ROJAS-DOTOR JULIA PÉREZ-RAMOS JUAN ANTONIO GIMÉNEZ-SCHERER FRANCISCO BLANCO-FAVELA GUADALUPE RICO-ROSILLO |
| author_facet | SARA ROJAS-DOTOR JULIA PÉREZ-RAMOS JUAN ANTONIO GIMÉNEZ-SCHERER FRANCISCO BLANCO-FAVELA GUADALUPE RICO-ROSILLO |
| author_sort | SARA ROJAS-DOTOR |
| collection | DOAJ |
| description | Entamoeba histolytica produces Monocyte Locomotion Inhibitory Factor (MLIF), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. Leukocytes are affected through the modulation of cytokine expression and/or production. We evaluated the effects of MLIF on the activation and production of intracellular cytokines in human CD4+ T lymphocytes by flow cytometry. Cells were stimulated for 24 h with PMA, MLIF, or PMA+MLIF. Cellular activation was measured using anti-CD69. Th1/Th2 production was studied by the expression of intracellular cytokines and cytokine/chemokine receptors. MLIF increased CD69 and induced the over-expression of the IL-lß, IFN-γ, IL-2, IL-4, and IL-10 intracellular cytokines; PMA+MLIF inhibited Th1 cytokine (IFN-γ) and increased Th2 cytokines (IL-4 and IL-10). The co-expression of the cytokine and chemokine receptors IFN-γ/CCR5 and IL-1ß/CCR5 was inhibited by PMA+MLIF and Th2 co-expression was increased. MLIF effects varied depending on the conditions. MLIF alone activated the Th1 and Th2 cytokines and cytokine/receptor expression; however, PMA+MLIF increased the expression of Th2 but inhibited it in Th1. |
| first_indexed | 2024-12-11T01:51:20Z |
| format | Article |
| id | doaj.art-735f7f1e90454c6e86b9580880f57de3 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-11T01:51:20Z |
| publishDate | 2009-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-735f7f1e90454c6e86b9580880f57de32022-12-22T01:24:44ZengBMCBiological Research0716-97600717-62872009-01-01424415425Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytesSARA ROJAS-DOTORJULIA PÉREZ-RAMOSJUAN ANTONIO GIMÉNEZ-SCHERERFRANCISCO BLANCO-FAVELAGUADALUPE RICO-ROSILLOEntamoeba histolytica produces Monocyte Locomotion Inhibitory Factor (MLIF), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. Leukocytes are affected through the modulation of cytokine expression and/or production. We evaluated the effects of MLIF on the activation and production of intracellular cytokines in human CD4+ T lymphocytes by flow cytometry. Cells were stimulated for 24 h with PMA, MLIF, or PMA+MLIF. Cellular activation was measured using anti-CD69. Th1/Th2 production was studied by the expression of intracellular cytokines and cytokine/chemokine receptors. MLIF increased CD69 and induced the over-expression of the IL-lß, IFN-γ, IL-2, IL-4, and IL-10 intracellular cytokines; PMA+MLIF inhibited Th1 cytokine (IFN-γ) and increased Th2 cytokines (IL-4 and IL-10). The co-expression of the cytokine and chemokine receptors IFN-γ/CCR5 and IL-1ß/CCR5 was inhibited by PMA+MLIF and Th2 co-expression was increased. MLIF effects varied depending on the conditions. MLIF alone activated the Th1 and Th2 cytokines and cytokine/receptor expression; however, PMA+MLIF increased the expression of Th2 but inhibited it in Th1.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400003Amebic hepatic abscess (AHA)CC chemokine receptors (CCR4, CCR5)monocyte locomotion inhibitory factor (MLIF)Entamoeba histolytica (E. histolytica)1-phorbol-12 myristate-13 acetate (PMA)interleukin (IL)T helper 1 lymphocyte (Th1)T helper 2 lymphocyte Th2) |
| spellingShingle | SARA ROJAS-DOTOR JULIA PÉREZ-RAMOS JUAN ANTONIO GIMÉNEZ-SCHERER FRANCISCO BLANCO-FAVELA GUADALUPE RICO-ROSILLO Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes Biological Research Amebic hepatic abscess (AHA) CC chemokine receptors (CCR4, CCR5) monocyte locomotion inhibitory factor (MLIF) Entamoeba histolytica (E. histolytica) 1-phorbol-12 myristate-13 acetate (PMA) interleukin (IL) T helper 1 lymphocyte (Th1) T helper 2 lymphocyte Th2) |
| title | Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes |
| title_full | Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes |
| title_fullStr | Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes |
| title_full_unstemmed | Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes |
| title_short | Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes |
| title_sort | effect of the monocyte locomotion inhibitory factor mlif produced by e histolityca on cytokines and chemokine receptors in t cd4 lymphocytes |
| topic | Amebic hepatic abscess (AHA) CC chemokine receptors (CCR4, CCR5) monocyte locomotion inhibitory factor (MLIF) Entamoeba histolytica (E. histolytica) 1-phorbol-12 myristate-13 acetate (PMA) interleukin (IL) T helper 1 lymphocyte (Th1) T helper 2 lymphocyte Th2) |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400003 |
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