Optimisation of lab-scale continuous alcohol-free beer production

In order to study the formation and conversion of the most important flavour compounds, the real wort used in alcohol-free beer fermentation was mimicked by a complex model medium containing glucose, yeast extract, and selected aldehydes. The fermentation experiments were carried out in a continuous...

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Main Authors: Radek Lehnert, Pavel Novák, Filipe Macieira, Michal Kuřec, José A. Teixeira, Tomáš Brányik
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2009-08-01
Series:Czech Journal of Food Sciences
Subjects:
Online Access:https://cjfs.agriculturejournals.cz/artkey/cjf-200904-0007_optimisation-of-lab-scale-continuous-alcohol-free-beer-production.php
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author Radek Lehnert
Pavel Novák
Filipe Macieira
Michal Kuřec
José A. Teixeira
Tomáš Brányik
author_facet Radek Lehnert
Pavel Novák
Filipe Macieira
Michal Kuřec
José A. Teixeira
Tomáš Brányik
author_sort Radek Lehnert
collection DOAJ
description In order to study the formation and conversion of the most important flavour compounds, the real wort used in alcohol-free beer fermentation was mimicked by a complex model medium containing glucose, yeast extract, and selected aldehydes. The fermentation experiments were carried out in a continuously operating gas-lift reactor with brewing yeast immobilised on spent grains (brewing by-product). During the continuous experiment, parameters such as oxygen supply, residence time (Rt), and temperature (T) were varied to find the optimal conditions for the alcohol-free beer production. The formation of ethanol, higher alcohols (HA), esters (ES), as well as the reduction of aldehydes and consumption of glucose were observed. The results suggest that the process parameters represent a powerful tool in controlling the degree of fermentation and flavour formation brought about by immobilised biocatalyst. Subsequently, the optimised process parameters were used to produce real alcohol-free beer during continuous fermentation. The final product was compared with batch fermented alcohol-free beers using the methods of instrumental and sensorial analysis.
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spelling doaj.art-7361e1955906481bbff5217ed4de72d82023-02-23T03:27:30ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172009-08-0127426727510.17221/128/2009-CJFScjf-200904-0007Optimisation of lab-scale continuous alcohol-free beer productionRadek Lehnert0Pavel Novák1Filipe Macieira2Michal Kuřec3José A. Teixeira4Tomáš Brányik5Department of Fermentation Chemistry and Bioengineering, Faculty of Food and Biochemical Technology, Institute of Chemical Technology, Prague, Czech RepublicDepartment of Fermentation Chemistry and Bioengineering, Faculty of Food and Biochemical Technology, Institute of Chemical Technology, Prague, Czech RepublicCentro de Engenharia Biológica, Universidade do Minho, Braga, PortugalDepartment of Fermentation Chemistry and Bioengineering, Faculty of Food and Biochemical Technology, Institute of Chemical Technology, Prague, Czech RepublicCentro de Engenharia Biológica, Universidade do Minho, Braga, PortugalDepartment of Fermentation Chemistry and Bioengineering, Faculty of Food and Biochemical Technology, Institute of Chemical Technology, Prague, Czech RepublicIn order to study the formation and conversion of the most important flavour compounds, the real wort used in alcohol-free beer fermentation was mimicked by a complex model medium containing glucose, yeast extract, and selected aldehydes. The fermentation experiments were carried out in a continuously operating gas-lift reactor with brewing yeast immobilised on spent grains (brewing by-product). During the continuous experiment, parameters such as oxygen supply, residence time (Rt), and temperature (T) were varied to find the optimal conditions for the alcohol-free beer production. The formation of ethanol, higher alcohols (HA), esters (ES), as well as the reduction of aldehydes and consumption of glucose were observed. The results suggest that the process parameters represent a powerful tool in controlling the degree of fermentation and flavour formation brought about by immobilised biocatalyst. Subsequently, the optimised process parameters were used to produce real alcohol-free beer during continuous fermentation. The final product was compared with batch fermented alcohol-free beers using the methods of instrumental and sensorial analysis.https://cjfs.agriculturejournals.cz/artkey/cjf-200904-0007_optimisation-of-lab-scale-continuous-alcohol-free-beer-production.phpalcohol-free beercontinuous reactorimmobilised yeastbeer flavour
spellingShingle Radek Lehnert
Pavel Novák
Filipe Macieira
Michal Kuřec
José A. Teixeira
Tomáš Brányik
Optimisation of lab-scale continuous alcohol-free beer production
Czech Journal of Food Sciences
alcohol-free beer
continuous reactor
immobilised yeast
beer flavour
title Optimisation of lab-scale continuous alcohol-free beer production
title_full Optimisation of lab-scale continuous alcohol-free beer production
title_fullStr Optimisation of lab-scale continuous alcohol-free beer production
title_full_unstemmed Optimisation of lab-scale continuous alcohol-free beer production
title_short Optimisation of lab-scale continuous alcohol-free beer production
title_sort optimisation of lab scale continuous alcohol free beer production
topic alcohol-free beer
continuous reactor
immobilised yeast
beer flavour
url https://cjfs.agriculturejournals.cz/artkey/cjf-200904-0007_optimisation-of-lab-scale-continuous-alcohol-free-beer-production.php
work_keys_str_mv AT radeklehnert optimisationoflabscalecontinuousalcoholfreebeerproduction
AT pavelnovak optimisationoflabscalecontinuousalcoholfreebeerproduction
AT filipemacieira optimisationoflabscalecontinuousalcoholfreebeerproduction
AT michalkurec optimisationoflabscalecontinuousalcoholfreebeerproduction
AT joseateixeira optimisationoflabscalecontinuousalcoholfreebeerproduction
AT tomasbranyik optimisationoflabscalecontinuousalcoholfreebeerproduction