Production of a Biopesticide Based on a Cysteine Protease Enzyme from Latex and Papaya (Carica Papaya) for Spodoptera Litura in Red Chili Peppers (Capsicum Annuum)
Spodoptera litura is one of the major pests on red chili peppers (Capsicum annuum). Larvae damage crops by biting, chewing, and then eating the lower surface of the leaves. The leaves became transparent white, and severe damage only leaves the leaf’s midrib and veins. Papaya latex (Carica papaya) co...
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2017-12-01
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| Series: | International Journal of Technology |
| Subjects: | |
| Online Access: | http://ijtech.eng.ui.ac.id/article/view/788 |
| _version_ | 1797970245097357312 |
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| author | Anondho Wijanarko Danti Firda Nur Muhamad Sahlan Nadia Tuada Afnan Tania Surya Utami Heri Hermansyah |
| author_facet | Anondho Wijanarko Danti Firda Nur Muhamad Sahlan Nadia Tuada Afnan Tania Surya Utami Heri Hermansyah |
| author_sort | Anondho Wijanarko |
| collection | DOAJ |
| description | Spodoptera litura is one of the major pests on red chili peppers (Capsicum annuum). Larvae damage crops by biting, chewing, and then eating the lower surface of the leaves. The leaves became transparent white, and severe damage only leaves the leaf’s midrib and veins. Papaya latex (Carica papaya) could be used as a pesticide because it contains cysteine protease, which is a substance that can inhibit the insects from eating the leaves or even kill the pests. The purpose of this study was to produce an organic pesticide from the cysteine protease extracted from papaya latex that is effective against Spodoptera litura. A completely randomized design was used with latex from papaya leaves, papaya rind, and papaya fruit. The methods used were blending and tapping. The blending method was conducted via a chemical extraction using buffer phosphate and ammonium sulfate. The tapping method was conducted via a chemical extraction using acetone. These methods were compared by using an enzyme activity test and efficacy test. The enzyme activity test used a UV-Vis spectrophotometer and the efficacy test was done on Spodoptera litura larvae, which were given red chili pepper leaf covered with an organic pesticide from cysteine protease. |
| first_indexed | 2024-04-11T03:13:52Z |
| format | Article |
| id | doaj.art-73643307b8af45cc82351235c5c44bf8 |
| institution | Directory Open Access Journal |
| issn | 2086-9614 2087-2100 |
| language | English |
| last_indexed | 2024-04-11T03:13:52Z |
| publishDate | 2017-12-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | International Journal of Technology |
| spelling | doaj.art-73643307b8af45cc82351235c5c44bf82023-01-02T10:48:08ZengUniversitas IndonesiaInternational Journal of Technology2086-96142087-21002017-12-01881455146110.14716/ijtech.v8i8.788788Production of a Biopesticide Based on a Cysteine Protease Enzyme from Latex and Papaya (Carica Papaya) for Spodoptera Litura in Red Chili Peppers (Capsicum Annuum)Anondho Wijanarko0Danti Firda Nur1Muhamad Sahlan2Nadia Tuada Afnan3Tania Surya Utami4Heri Hermansyah5Universitas IndonesiaUniversitas IndonesiaUniversitas IndonesiaUniversitas IndonesiaUniversitas Indonesia- Chemical Engineering Department, Universitas Indonesia, Depok, Indonesia<br/>-Spodoptera litura is one of the major pests on red chili peppers (Capsicum annuum). Larvae damage crops by biting, chewing, and then eating the lower surface of the leaves. The leaves became transparent white, and severe damage only leaves the leaf’s midrib and veins. Papaya latex (Carica papaya) could be used as a pesticide because it contains cysteine protease, which is a substance that can inhibit the insects from eating the leaves or even kill the pests. The purpose of this study was to produce an organic pesticide from the cysteine protease extracted from papaya latex that is effective against Spodoptera litura. A completely randomized design was used with latex from papaya leaves, papaya rind, and papaya fruit. The methods used were blending and tapping. The blending method was conducted via a chemical extraction using buffer phosphate and ammonium sulfate. The tapping method was conducted via a chemical extraction using acetone. These methods were compared by using an enzyme activity test and efficacy test. The enzyme activity test used a UV-Vis spectrophotometer and the efficacy test was done on Spodoptera litura larvae, which were given red chili pepper leaf covered with an organic pesticide from cysteine protease.http://ijtech.eng.ui.ac.id/article/view/788Cysteine proteasePapaya latexRed chili pepperSpodoptera litura |
| spellingShingle | Anondho Wijanarko Danti Firda Nur Muhamad Sahlan Nadia Tuada Afnan Tania Surya Utami Heri Hermansyah Production of a Biopesticide Based on a Cysteine Protease Enzyme from Latex and Papaya (Carica Papaya) for Spodoptera Litura in Red Chili Peppers (Capsicum Annuum) International Journal of Technology Cysteine protease Papaya latex Red chili pepper Spodoptera litura |
| title | Production of a Biopesticide Based on a Cysteine Protease Enzyme from Latex and Papaya (Carica Papaya) for Spodoptera Litura in Red Chili Peppers (Capsicum Annuum) |
| title_full | Production of a Biopesticide Based on a Cysteine Protease Enzyme from Latex and Papaya (Carica Papaya) for Spodoptera Litura in Red Chili Peppers (Capsicum Annuum) |
| title_fullStr | Production of a Biopesticide Based on a Cysteine Protease Enzyme from Latex and Papaya (Carica Papaya) for Spodoptera Litura in Red Chili Peppers (Capsicum Annuum) |
| title_full_unstemmed | Production of a Biopesticide Based on a Cysteine Protease Enzyme from Latex and Papaya (Carica Papaya) for Spodoptera Litura in Red Chili Peppers (Capsicum Annuum) |
| title_short | Production of a Biopesticide Based on a Cysteine Protease Enzyme from Latex and Papaya (Carica Papaya) for Spodoptera Litura in Red Chili Peppers (Capsicum Annuum) |
| title_sort | production of a biopesticide based on a cysteine protease enzyme from latex and papaya carica papaya for spodoptera litura in red chili peppers capsicum annuum |
| topic | Cysteine protease Papaya latex Red chili pepper Spodoptera litura |
| url | http://ijtech.eng.ui.ac.id/article/view/788 |
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