Quantitative image analysis of thrombus formation in microfluidic in-vitro models
Abstract In this study, we present a method to quantitatively analyze the thrombus formation process through image analysis in an in vitro thrombus model with a circular cross section. The thrombus model used was designed based on the mechanism between the physical principle of wall shear rate (WSR)...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-12-01
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| Series: | Micro and Nano Systems Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40486-022-00166-3 |
| _version_ | 1797977342353604608 |
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| author | Ji-Seob Choi Dong-Hwi Ham Jung-Hyun Kim Helem Betsua Flores Marcial Pyeong-Ho Jeong Jin-Ho Choi Woo-Tae Park |
| author_facet | Ji-Seob Choi Dong-Hwi Ham Jung-Hyun Kim Helem Betsua Flores Marcial Pyeong-Ho Jeong Jin-Ho Choi Woo-Tae Park |
| author_sort | Ji-Seob Choi |
| collection | DOAJ |
| description | Abstract In this study, we present a method to quantitatively analyze the thrombus formation process through image analysis in an in vitro thrombus model with a circular cross section. The thrombus model used was designed based on the mechanism between the physical principle of wall shear rate (WSR) and thrombus formation. Image analysis was used to help visualize the thrombus formation process and calculate the thrombus area. Through this method, the thrombus formation and growth from the channel wall was demonstrated without the use of fluorescence. In addition, by dividing the image into sub-sections, the accuracy of the thrombus growth pattern was improved. The departing blood clots which are called embolus, were observed being separated from the thrombus. |
| first_indexed | 2024-04-11T05:05:16Z |
| format | Article |
| id | doaj.art-73648dabb886480d95afa4af3f8e1a71 |
| institution | Directory Open Access Journal |
| issn | 2213-9621 |
| language | English |
| last_indexed | 2024-04-11T05:05:16Z |
| publishDate | 2022-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Micro and Nano Systems Letters |
| spelling | doaj.art-73648dabb886480d95afa4af3f8e1a712022-12-25T12:25:11ZengSpringerOpenMicro and Nano Systems Letters2213-96212022-12-011011710.1186/s40486-022-00166-3Quantitative image analysis of thrombus formation in microfluidic in-vitro modelsJi-Seob Choi0Dong-Hwi Ham1Jung-Hyun Kim2Helem Betsua Flores Marcial3Pyeong-Ho Jeong4Jin-Ho Choi5Woo-Tae Park6Department of Mechanical Engineering, Seoul National University of Science and TechnologyDepartment of Mechanical Engineering, Seoul National University of Science and TechnologyDepartment of Mechanical Engineering, Seoul National University of Science and TechnologyConvergence Institute of Biomedical Engineering and Biomaterials, Seoul National University of Science and TechnologyDepartment of Mechanical Engineering, Seoul National University of Science and TechnologySamsung Medical CenterDepartment of Mechanical Engineering, Seoul National University of Science and TechnologyAbstract In this study, we present a method to quantitatively analyze the thrombus formation process through image analysis in an in vitro thrombus model with a circular cross section. The thrombus model used was designed based on the mechanism between the physical principle of wall shear rate (WSR) and thrombus formation. Image analysis was used to help visualize the thrombus formation process and calculate the thrombus area. Through this method, the thrombus formation and growth from the channel wall was demonstrated without the use of fluorescence. In addition, by dividing the image into sub-sections, the accuracy of the thrombus growth pattern was improved. The departing blood clots which are called embolus, were observed being separated from the thrombus.https://doi.org/10.1186/s40486-022-00166-3ThrombosisThrombus formationEmbolusImage analysisPlatelet |
| spellingShingle | Ji-Seob Choi Dong-Hwi Ham Jung-Hyun Kim Helem Betsua Flores Marcial Pyeong-Ho Jeong Jin-Ho Choi Woo-Tae Park Quantitative image analysis of thrombus formation in microfluidic in-vitro models Micro and Nano Systems Letters Thrombosis Thrombus formation Embolus Image analysis Platelet |
| title | Quantitative image analysis of thrombus formation in microfluidic in-vitro models |
| title_full | Quantitative image analysis of thrombus formation in microfluidic in-vitro models |
| title_fullStr | Quantitative image analysis of thrombus formation in microfluidic in-vitro models |
| title_full_unstemmed | Quantitative image analysis of thrombus formation in microfluidic in-vitro models |
| title_short | Quantitative image analysis of thrombus formation in microfluidic in-vitro models |
| title_sort | quantitative image analysis of thrombus formation in microfluidic in vitro models |
| topic | Thrombosis Thrombus formation Embolus Image analysis Platelet |
| url | https://doi.org/10.1186/s40486-022-00166-3 |
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