Antifungal Susceptibility of 3,4-di-hydropyrimidine-1-(H2)-L-H1-pyrrole Derivatives in Candida Clinical Isolates
Background and purpose: There is an increasing rate of drug resistance to azole among Candida species, so, finding new compounds that are effective in laboratory conditions, such as 3,4-dihydropyrimidine derivatives are important. The purpose of this study was to evaluate the antifungal sensitivity...
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| Format: | Article |
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Mazandaran University of Medical Sciences
2022-12-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
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| Online Access: | http://jmums.mazums.ac.ir/article-1-18712-en.html |
| _version_ | 1797893154970533888 |
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| author | Khosro Ghashari Saeid Mahdavi Omran Asieh Khalilpur Jalal Jafarzadeh Mojtaba Taghizadeh Armaki Akbar Hossein Nejad Ahmad Reza Aminian |
| author_facet | Khosro Ghashari Saeid Mahdavi Omran Asieh Khalilpur Jalal Jafarzadeh Mojtaba Taghizadeh Armaki Akbar Hossein Nejad Ahmad Reza Aminian |
| author_sort | Khosro Ghashari |
| collection | DOAJ |
| description | Background and purpose: There is an increasing rate of drug resistance to azole among Candida species, so, finding new compounds that are effective in laboratory conditions, such as 3,4-dihydropyrimidine derivatives are important. The purpose of this study was to evaluate the antifungal sensitivity of 3,4-di-hydropyrimidine-1- (H2) -L- H1-pyrrole derivatives in Candida isolates.
Materials and methods: Antifungal sensitivity of 102 Candida isolates with the origin of otomycosis to dihydropyrimidine derivatives and itraconazole were evaluated by broth microdilution according to CLSI-M27S4 guidelines. The serial dilution range of compounds and antifungal drug was 0.016-16 μg/ml. A concentration of compounds that showed at least 50% growth inhibition compared to the positive control group was considered as the minimum inhibitory concentration (MIC). Statistical analysis was performed in SPSS V16.
Results: Findings showed that 3,4-di-hydropyrimidine-1-(H2)-L-H1-pyrrole derivatives have higher MIC than itraconazole against Candida species. Also, comparing the MIC values of 3,4-di- hydropyrimidine with each other (P1-P4), P1 derivatives were found with lower MIC values than the other three derivatives and almost all compounds showed more efficacy against Candida albicans than other Candida species.
Conclusion: Although the antifungal effects of 3,4-dihydropyrimidine-1-(H2)-L-H1-pyrrole derivatives against Candida species were lower than itraconazole, but, making structural changes in these compounds can increase their antifungal effects. |
| first_indexed | 2024-04-10T06:48:24Z |
| format | Article |
| id | doaj.art-7365198baf5e495387f6220dcf8a9618 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T06:48:24Z |
| publishDate | 2022-12-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-7365198baf5e495387f6220dcf8a96182023-02-28T09:18:48ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792022-12-01322152634Antifungal Susceptibility of 3,4-di-hydropyrimidine-1-(H2)-L-H1-pyrrole Derivatives in Candida Clinical IsolatesKhosro Ghashari0Saeid Mahdavi Omran1Asieh Khalilpur2Jalal Jafarzadeh3Mojtaba Taghizadeh Armaki4Akbar Hossein Nejad5Ahmad Reza Aminian6 General Practitioner, Student Research Committee, Babol University of Medical Sciences, Babol, Iran Professor, Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Assistant Professor, Environmental Health Research Center, Social Determinants of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran MSc in Medical Mycology, Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Assistant Professor, Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran PhD Student in Medical Mycology, Faculty of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran PhD Student in Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Background and purpose: There is an increasing rate of drug resistance to azole among Candida species, so, finding new compounds that are effective in laboratory conditions, such as 3,4-dihydropyrimidine derivatives are important. The purpose of this study was to evaluate the antifungal sensitivity of 3,4-di-hydropyrimidine-1- (H2) -L- H1-pyrrole derivatives in Candida isolates. Materials and methods: Antifungal sensitivity of 102 Candida isolates with the origin of otomycosis to dihydropyrimidine derivatives and itraconazole were evaluated by broth microdilution according to CLSI-M27S4 guidelines. The serial dilution range of compounds and antifungal drug was 0.016-16 μg/ml. A concentration of compounds that showed at least 50% growth inhibition compared to the positive control group was considered as the minimum inhibitory concentration (MIC). Statistical analysis was performed in SPSS V16. Results: Findings showed that 3,4-di-hydropyrimidine-1-(H2)-L-H1-pyrrole derivatives have higher MIC than itraconazole against Candida species. Also, comparing the MIC values of 3,4-di- hydropyrimidine with each other (P1-P4), P1 derivatives were found with lower MIC values than the other three derivatives and almost all compounds showed more efficacy against Candida albicans than other Candida species. Conclusion: Although the antifungal effects of 3,4-dihydropyrimidine-1-(H2)-L-H1-pyrrole derivatives against Candida species were lower than itraconazole, but, making structural changes in these compounds can increase their antifungal effects.http://jmums.mazums.ac.ir/article-1-18712-en.html34-di-hydropyrimidine-1-(h2)-l-h1-pyrrole derivativesitraconazolecandida species |
| spellingShingle | Khosro Ghashari Saeid Mahdavi Omran Asieh Khalilpur Jalal Jafarzadeh Mojtaba Taghizadeh Armaki Akbar Hossein Nejad Ahmad Reza Aminian Antifungal Susceptibility of 3,4-di-hydropyrimidine-1-(H2)-L-H1-pyrrole Derivatives in Candida Clinical Isolates Journal of Mazandaran University of Medical Sciences 3 4-di-hydropyrimidine-1-(h2)-l-h1-pyrrole derivatives itraconazole candida species |
| title | Antifungal Susceptibility of 3,4-di-hydropyrimidine-1-(H2)-L-H1-pyrrole Derivatives in Candida Clinical Isolates |
| title_full | Antifungal Susceptibility of 3,4-di-hydropyrimidine-1-(H2)-L-H1-pyrrole Derivatives in Candida Clinical Isolates |
| title_fullStr | Antifungal Susceptibility of 3,4-di-hydropyrimidine-1-(H2)-L-H1-pyrrole Derivatives in Candida Clinical Isolates |
| title_full_unstemmed | Antifungal Susceptibility of 3,4-di-hydropyrimidine-1-(H2)-L-H1-pyrrole Derivatives in Candida Clinical Isolates |
| title_short | Antifungal Susceptibility of 3,4-di-hydropyrimidine-1-(H2)-L-H1-pyrrole Derivatives in Candida Clinical Isolates |
| title_sort | antifungal susceptibility of 3 4 di hydropyrimidine 1 h2 l h1 pyrrole derivatives in candida clinical isolates |
| topic | 3 4-di-hydropyrimidine-1-(h2)-l-h1-pyrrole derivatives itraconazole candida species |
| url | http://jmums.mazums.ac.ir/article-1-18712-en.html |
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