Xylitol Production by <i>Debaryomyces hansenii</i> in Extracted Olive Pomace Dilute-Acid Hydrolysate
The extracted olive pomace (EOP) is an industrial lignocellulosic by-product of olive pomace oil extraction, currently mainly used for energy production through combustion. In this work, the hemicellulosic fraction of EOP was selectively hydrolyzed by diluted acid hydrolysis to obtain pentose-rich h...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/12/1020 |
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| author | Ana Rita C. Morais Luís C. Duarte Pedro Lourenço Ivone Torrado Teresa Brás Luísa A. Neves Florbela Carvalheiro |
| author_facet | Ana Rita C. Morais Luís C. Duarte Pedro Lourenço Ivone Torrado Teresa Brás Luísa A. Neves Florbela Carvalheiro |
| author_sort | Ana Rita C. Morais |
| collection | DOAJ |
| description | The extracted olive pomace (EOP) is an industrial lignocellulosic by-product of olive pomace oil extraction, currently mainly used for energy production through combustion. In this work, the hemicellulosic fraction of EOP was selectively hydrolyzed by diluted acid hydrolysis to obtain pentose-rich hydrolysates that can potentially be upgraded by <i>Debaryomyces hansenii</i>, targeting xylitol production. The monosaccharides and degradation by-products released along the pre-treatment were quantified and several detoxification methods for the removal of potentially toxic compounds were evaluated, including pH adjustment to 5.5, the use of anion-exchange resins, adsorption into activated charcoal, concentration by evaporation, and membrane techniques, i.e., nanofiltration. The latter approach was shown to be the best method allowing the full removal of furfural, 41% of 5-hydroxymethylfurfural, 54% of acetic acid, and 67% of the phenolic compounds present in the hydrolysate. The effects of the supplementation of both non-detoxified and detoxified hydrolysates were also assessed. The non-detoxified hydrolysate, under aerobic conditions, supported the yeast growth and xylitol production at low levels. Supplementation with the low-cost corn steep liquor of the nanofiltration detoxified hydrolysate showed a higher xylitol yield (0.57 g/g) compared to the non-detoxified hydrolysate. The highest xylitol productivity was found in hydrolysate detoxified with anionic resins (0.30 g/L·h), which was 80% higher than in the non-detoxified culture medium. Overall, the results showed that EOP dilute acid hydrolysates can efficiently be used for xylitol production by <i>D. hansenii</i> if detoxification, and supplementation, even with low-cost supplements, are performed. |
| first_indexed | 2024-03-08T20:46:49Z |
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| id | doaj.art-736542cbfdcc495987ac11984c555c2e |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-08T20:46:49Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-736542cbfdcc495987ac11984c555c2e2023-12-22T14:07:55ZengMDPI AGFermentation2311-56372023-12-01912102010.3390/fermentation9121020Xylitol Production by <i>Debaryomyces hansenii</i> in Extracted Olive Pomace Dilute-Acid HydrolysateAna Rita C. Morais0Luís C. Duarte1Pedro Lourenço2Ivone Torrado3Teresa Brás4Luísa A. Neves5Florbela Carvalheiro6Unidade de Bioenergia e Biorrefinarias, LNEG—Laboratório Nacional de Energia e Geologia, I.P, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, PortugalUnidade de Bioenergia e Biorrefinarias, LNEG—Laboratório Nacional de Energia e Geologia, I.P, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, PortugalUCASUL—União de Cooperativas UCRL, 7920-201 Alvito, PortugalUnidade de Bioenergia e Biorrefinarias, LNEG—Laboratório Nacional de Energia e Geologia, I.P, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, PortugalAlentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)/Polytechnic Institute of Beja (IPBeja), 7801-908 Beja, PortugalLAQV/REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade NOVA de Lisboa, Campus da Caparica, 2829-516 Almada, PortugalUnidade de Bioenergia e Biorrefinarias, LNEG—Laboratório Nacional de Energia e Geologia, I.P, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, PortugalThe extracted olive pomace (EOP) is an industrial lignocellulosic by-product of olive pomace oil extraction, currently mainly used for energy production through combustion. In this work, the hemicellulosic fraction of EOP was selectively hydrolyzed by diluted acid hydrolysis to obtain pentose-rich hydrolysates that can potentially be upgraded by <i>Debaryomyces hansenii</i>, targeting xylitol production. The monosaccharides and degradation by-products released along the pre-treatment were quantified and several detoxification methods for the removal of potentially toxic compounds were evaluated, including pH adjustment to 5.5, the use of anion-exchange resins, adsorption into activated charcoal, concentration by evaporation, and membrane techniques, i.e., nanofiltration. The latter approach was shown to be the best method allowing the full removal of furfural, 41% of 5-hydroxymethylfurfural, 54% of acetic acid, and 67% of the phenolic compounds present in the hydrolysate. The effects of the supplementation of both non-detoxified and detoxified hydrolysates were also assessed. The non-detoxified hydrolysate, under aerobic conditions, supported the yeast growth and xylitol production at low levels. Supplementation with the low-cost corn steep liquor of the nanofiltration detoxified hydrolysate showed a higher xylitol yield (0.57 g/g) compared to the non-detoxified hydrolysate. The highest xylitol productivity was found in hydrolysate detoxified with anionic resins (0.30 g/L·h), which was 80% higher than in the non-detoxified culture medium. Overall, the results showed that EOP dilute acid hydrolysates can efficiently be used for xylitol production by <i>D. hansenii</i> if detoxification, and supplementation, even with low-cost supplements, are performed.https://www.mdpi.com/2311-5637/9/12/1020<i>Debaryomyces hansenii</i>detoxificationextracted olive pomacepentose-rich hydrolysatesupplementation |
| spellingShingle | Ana Rita C. Morais Luís C. Duarte Pedro Lourenço Ivone Torrado Teresa Brás Luísa A. Neves Florbela Carvalheiro Xylitol Production by <i>Debaryomyces hansenii</i> in Extracted Olive Pomace Dilute-Acid Hydrolysate Fermentation <i>Debaryomyces hansenii</i> detoxification extracted olive pomace pentose-rich hydrolysate supplementation |
| title | Xylitol Production by <i>Debaryomyces hansenii</i> in Extracted Olive Pomace Dilute-Acid Hydrolysate |
| title_full | Xylitol Production by <i>Debaryomyces hansenii</i> in Extracted Olive Pomace Dilute-Acid Hydrolysate |
| title_fullStr | Xylitol Production by <i>Debaryomyces hansenii</i> in Extracted Olive Pomace Dilute-Acid Hydrolysate |
| title_full_unstemmed | Xylitol Production by <i>Debaryomyces hansenii</i> in Extracted Olive Pomace Dilute-Acid Hydrolysate |
| title_short | Xylitol Production by <i>Debaryomyces hansenii</i> in Extracted Olive Pomace Dilute-Acid Hydrolysate |
| title_sort | xylitol production by i debaryomyces hansenii i in extracted olive pomace dilute acid hydrolysate |
| topic | <i>Debaryomyces hansenii</i> detoxification extracted olive pomace pentose-rich hydrolysate supplementation |
| url | https://www.mdpi.com/2311-5637/9/12/1020 |
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