Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues
Abstract Background The incidence of inflammatory bowel disease (IBD), an inflammatory disorder of the gastrointestinal system has increased. IBD, characterized by aberrant immune responses against antigens, is thought to be caused by the invasion of enterobacteria. The pathogenesis of IBD is compli...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12906-022-03536-x |
| _version_ | 1818137629167714304 |
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| author | Ji Min Shin Yang-Ju Son In Jin Ha Saruul Erdenebileg Da Seul Jung Dae-geun Song Young Sik Kim Sang Min Kim Chu Won Nho |
| author_facet | Ji Min Shin Yang-Ju Son In Jin Ha Saruul Erdenebileg Da Seul Jung Dae-geun Song Young Sik Kim Sang Min Kim Chu Won Nho |
| author_sort | Ji Min Shin |
| collection | DOAJ |
| description | Abstract Background The incidence of inflammatory bowel disease (IBD), an inflammatory disorder of the gastrointestinal system has increased. IBD, characterized by aberrant immune responses against antigens, is thought to be caused by the invasion of enterobacteria. The pathogenesis of IBD is complicated, hence novel effective therapeutic agents are warranted. Therefore, this study evaluates the potential of Artemisia argyi, a medicinal herb, in alleviating IBD. Methods The effectiveness of the A. argyi ethanol extract was verified both in vitro and in vivo. Inflammation was induced in RAW 264.7 cells by 1 μg/mL of lipopolysaccharide (LPS) and by 3% dextran sodium sulfate (DSS) in a DSS-induced colitis mouse model. During the ten-day colitis induction, 200 mg/kg of A. argyi ethanol extract was orally administered to the treatment group. Levels of inflammation-related proteins and genes were analyzed in the colon, serum, and lymphoid tissues, i.e., Peyer’s patches (PPs) and spleen. The chemical constituent of the A. argyi ethanol extract was identified using an ultra-high performance liquid chromatography mass spectrometry (UPLC-MS/MS) analysis. Results A. argyi ethanol extract treatment ameliorated IBD symptoms and reduced the expression of inflammation-related proteins and genes in the colon and serum samples. Furthermore, A. argyi treatment induced the activation of anti-oxidative associated proteins, such as nuclear factor-erythroid factor 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1); and the treatment have also inhibited nuclear factor-κB (NF-κB), a central mediator of inflammatory responses. A. argyi enhanced the immunomodulatory effects in the PPs and spleen, which may stem from interleukin-10 (IL-10) upregulation. Chemical analysis identified a total of 28 chemical compounds, several of which have been reported to exert anti-inflammatory effects. Conclusions The effectiveness of the A. argyi ethanol extract in alleviating IBD was demonstrated; application of the extract successfully mitigated IBD symptoms, and enhanced immunomodulatory responses in lymphoid tissues. These findings suggest A. argyi as a promising herbal medicine for IBD treatment. |
| first_indexed | 2024-12-11T09:59:20Z |
| format | Article |
| id | doaj.art-736c5d5509ef45d5ad729c5cae7427b1 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-11T09:59:20Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-736c5d5509ef45d5ad729c5cae7427b12022-12-22T01:12:09ZengBMCBMC Complementary Medicine and Therapies2662-76712022-03-0122111610.1186/s12906-022-03536-xArtemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissuesJi Min Shin0Yang-Ju Son1In Jin Ha2Saruul Erdenebileg3Da Seul Jung4Dae-geun Song5Young Sik Kim6Sang Min Kim7Chu Won Nho8Smart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural ProductsDepartment of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang UniversityKorean Medicine Clinical Trial Center (K-CTC), Kyung Hee University Korean Medicine HospitalSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural ProductsSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural ProductsNatural Products Informatics Center, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural ProductsCollege of Pharmacy, Natural Products Research Institute, Seoul National UniversitySmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural ProductsSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural ProductsAbstract Background The incidence of inflammatory bowel disease (IBD), an inflammatory disorder of the gastrointestinal system has increased. IBD, characterized by aberrant immune responses against antigens, is thought to be caused by the invasion of enterobacteria. The pathogenesis of IBD is complicated, hence novel effective therapeutic agents are warranted. Therefore, this study evaluates the potential of Artemisia argyi, a medicinal herb, in alleviating IBD. Methods The effectiveness of the A. argyi ethanol extract was verified both in vitro and in vivo. Inflammation was induced in RAW 264.7 cells by 1 μg/mL of lipopolysaccharide (LPS) and by 3% dextran sodium sulfate (DSS) in a DSS-induced colitis mouse model. During the ten-day colitis induction, 200 mg/kg of A. argyi ethanol extract was orally administered to the treatment group. Levels of inflammation-related proteins and genes were analyzed in the colon, serum, and lymphoid tissues, i.e., Peyer’s patches (PPs) and spleen. The chemical constituent of the A. argyi ethanol extract was identified using an ultra-high performance liquid chromatography mass spectrometry (UPLC-MS/MS) analysis. Results A. argyi ethanol extract treatment ameliorated IBD symptoms and reduced the expression of inflammation-related proteins and genes in the colon and serum samples. Furthermore, A. argyi treatment induced the activation of anti-oxidative associated proteins, such as nuclear factor-erythroid factor 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1); and the treatment have also inhibited nuclear factor-κB (NF-κB), a central mediator of inflammatory responses. A. argyi enhanced the immunomodulatory effects in the PPs and spleen, which may stem from interleukin-10 (IL-10) upregulation. Chemical analysis identified a total of 28 chemical compounds, several of which have been reported to exert anti-inflammatory effects. Conclusions The effectiveness of the A. argyi ethanol extract in alleviating IBD was demonstrated; application of the extract successfully mitigated IBD symptoms, and enhanced immunomodulatory responses in lymphoid tissues. These findings suggest A. argyi as a promising herbal medicine for IBD treatment.https://doi.org/10.1186/s12906-022-03536-xInflammatory bowel diseasesArtemisia argyiNatural productsSpleenUPLC-MSMS |
| spellingShingle | Ji Min Shin Yang-Ju Son In Jin Ha Saruul Erdenebileg Da Seul Jung Dae-geun Song Young Sik Kim Sang Min Kim Chu Won Nho Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues BMC Complementary Medicine and Therapies Inflammatory bowel diseases Artemisia argyi Natural products Spleen UPLC-MS MS |
| title | Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues |
| title_full | Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues |
| title_fullStr | Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues |
| title_full_unstemmed | Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues |
| title_short | Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues |
| title_sort | artemisia argyi extract alleviates inflammation in a dss induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues |
| topic | Inflammatory bowel diseases Artemisia argyi Natural products Spleen UPLC-MS MS |
| url | https://doi.org/10.1186/s12906-022-03536-x |
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