Isolation and evolutionary analyses of porcine epidemic diarrhea virus in Asia
Porcine epidemic diarrhea virus (PEDV) is a leading cause of diarrhea in pigs worldwide. Virus isolation and genetic evolutionary analysis allow investigations into the prevalence of epidemic strains and provide data for the clinical diagnosis and vaccine development. In this study, we investigated...
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PeerJ Inc.
2020-10-01
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| Online Access: | https://peerj.com/articles/10114.pdf |
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| author | Wan Liang Danna Zhou Chao Geng Keli Yang Zhengying Duan Rui Guo Wei Liu Fangyan Yuan Zewen Liu Ting Gao Ling Zhao Dongwan Yoo Yongxiang Tian |
| author_facet | Wan Liang Danna Zhou Chao Geng Keli Yang Zhengying Duan Rui Guo Wei Liu Fangyan Yuan Zewen Liu Ting Gao Ling Zhao Dongwan Yoo Yongxiang Tian |
| author_sort | Wan Liang |
| collection | DOAJ |
| description | Porcine epidemic diarrhea virus (PEDV) is a leading cause of diarrhea in pigs worldwide. Virus isolation and genetic evolutionary analysis allow investigations into the prevalence of epidemic strains and provide data for the clinical diagnosis and vaccine development. In this study, we investigated the genetic characteristics of PEDV circulation in Asia through virus isolation and comparative genomics analysis. APEDV strain designated HB2018 was isolated from a pig in a farm experiencing a diarrhea outbreak. The complete genome sequence of HB2018 was 28,138 bp in length. Phylogenetic analysis of HB2018 and 207 PEDVs in Asia showed that most PEDV strains circulating in Asia after 2010 belong to genotype GII, particularly GII-a. The PEDV vaccine strain CV777 belonged to GI, and thus, unmatched genotypes between CV777 and GII-a variants might partially explain incomplete protection by the CV777-derived vaccine against PEDV variants in China. In addition, we found the S protein of variant strains contained numerous mutations compared to the S protein of CV777, and these mutations occurred in the N-terminal domain of the S protein. These mutations may influence the antigenicity, pathogenicity, and neutralization properties of the variant strains. |
| first_indexed | 2024-03-09T07:02:15Z |
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| id | doaj.art-736d937b3e4140b09c75a64659b87315 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:02:15Z |
| publishDate | 2020-10-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
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| spelling | doaj.art-736d937b3e4140b09c75a64659b873152023-12-03T09:51:06ZengPeerJ Inc.PeerJ2167-83592020-10-018e1011410.7717/peerj.10114Isolation and evolutionary analyses of porcine epidemic diarrhea virus in AsiaWan Liang0Danna Zhou1Chao Geng2Keli Yang3Zhengying Duan4Rui Guo5Wei Liu6Fangyan Yuan7Zewen Liu8Ting Gao9Ling Zhao10Dongwan Yoo11Yongxiang Tian12Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaDepartment of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Champaign, United States of AmericaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, ChinaPorcine epidemic diarrhea virus (PEDV) is a leading cause of diarrhea in pigs worldwide. Virus isolation and genetic evolutionary analysis allow investigations into the prevalence of epidemic strains and provide data for the clinical diagnosis and vaccine development. In this study, we investigated the genetic characteristics of PEDV circulation in Asia through virus isolation and comparative genomics analysis. APEDV strain designated HB2018 was isolated from a pig in a farm experiencing a diarrhea outbreak. The complete genome sequence of HB2018 was 28,138 bp in length. Phylogenetic analysis of HB2018 and 207 PEDVs in Asia showed that most PEDV strains circulating in Asia after 2010 belong to genotype GII, particularly GII-a. The PEDV vaccine strain CV777 belonged to GI, and thus, unmatched genotypes between CV777 and GII-a variants might partially explain incomplete protection by the CV777-derived vaccine against PEDV variants in China. In addition, we found the S protein of variant strains contained numerous mutations compared to the S protein of CV777, and these mutations occurred in the N-terminal domain of the S protein. These mutations may influence the antigenicity, pathogenicity, and neutralization properties of the variant strains.https://peerj.com/articles/10114.pdfPorcine epidemic diarrhea virusVirus isolationGenetic evolutionary analysisAmino acid mutationsAsia |
| spellingShingle | Wan Liang Danna Zhou Chao Geng Keli Yang Zhengying Duan Rui Guo Wei Liu Fangyan Yuan Zewen Liu Ting Gao Ling Zhao Dongwan Yoo Yongxiang Tian Isolation and evolutionary analyses of porcine epidemic diarrhea virus in Asia PeerJ Porcine epidemic diarrhea virus Virus isolation Genetic evolutionary analysis Amino acid mutations Asia |
| title | Isolation and evolutionary analyses of porcine epidemic diarrhea virus in Asia |
| title_full | Isolation and evolutionary analyses of porcine epidemic diarrhea virus in Asia |
| title_fullStr | Isolation and evolutionary analyses of porcine epidemic diarrhea virus in Asia |
| title_full_unstemmed | Isolation and evolutionary analyses of porcine epidemic diarrhea virus in Asia |
| title_short | Isolation and evolutionary analyses of porcine epidemic diarrhea virus in Asia |
| title_sort | isolation and evolutionary analyses of porcine epidemic diarrhea virus in asia |
| topic | Porcine epidemic diarrhea virus Virus isolation Genetic evolutionary analysis Amino acid mutations Asia |
| url | https://peerj.com/articles/10114.pdf |
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