Detection of <i>mcr-1</i> Gene in Undefined <i>Vibrio</i> Species Isolated from Clams
The increase of antimicrobial resistant strains is leading to an emerging threat to public health. Pathogenic <i>Vibrio</i> are responsible for human and animal illness. The Enterobacteriaceae family includes microorganisms that affect humans, causing several infections. One of the main...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/2/394 |
| _version_ | 1797477794493497344 |
|---|---|
| author | Christian Valdez Cátia Costa Marco Simões Carla C. C. R. de Carvalho Teresa Baptista Maria J. Campos |
| author_facet | Christian Valdez Cátia Costa Marco Simões Carla C. C. R. de Carvalho Teresa Baptista Maria J. Campos |
| author_sort | Christian Valdez |
| collection | DOAJ |
| description | The increase of antimicrobial resistant strains is leading to an emerging threat to public health. Pathogenic <i>Vibrio</i> are responsible for human and animal illness. The Enterobacteriaceae family includes microorganisms that affect humans, causing several infections. One of the main causes of human infection is related to the ingestion of undercooked seafood. Due to their filter-feeding habit, marine invertebrates, such as clams, are known to be a natural reservoir of specific microbial communities. In the present study, Vibrionaceae and coliforms microorganisms were isolated from clams. A microbial susceptibility test was performed using the disk diffusion method. From 43 presumptive <i>Vibrio</i> spp. and 17 coliforms, three <i>Vibrio</i> spp. with MICs to colistin >512 mg L<sup>−1</sup> were found. From the 23 antimicrobial resistance genes investigated, only the three isolates that showed phenotypic resistance to colistin contained the <i>mcr-1</i> gene. Genotypic analysis for virulence genes in EB07V indicated <i>chiA</i> gene presence. The results from the plasmid cure and transformation showed that the resistance is chromosomally mediated. Biochemical analysis and MLSA, on the basis of four protein-coding gene sequences (<i>recA, rpoB</i>, <i>groEL</i> and <i>dnaJ</i>), grouped the isolates into the genus <i>Vibrio</i> but distinguished them as different from any known <i>Vibrio</i> spp. |
| first_indexed | 2024-03-09T21:23:43Z |
| format | Article |
| id | doaj.art-736e65fa85724523865f1330f6ce3a9e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:23:43Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-736e65fa85724523865f1330f6ce3a9e2023-11-23T21:15:29ZengMDPI AGMicroorganisms2076-26072022-02-0110239410.3390/microorganisms10020394Detection of <i>mcr-1</i> Gene in Undefined <i>Vibrio</i> Species Isolated from ClamsChristian Valdez0Cátia Costa1Marco Simões2Carla C. C. R. de Carvalho3Teresa Baptista4Maria J. Campos5MARE-Marine and Environmental Sciences Centre, ESTM, Polytechnic of Leiria, 2520-630 Peniche, PortugalMARE-Marine and Environmental Sciences Centre, ESTM, Polytechnic of Leiria, 2520-630 Peniche, PortugalMARE-Marine and Environmental Sciences Centre, ESTM, Polytechnic of Leiria, 2520-630 Peniche, PortugaliBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Associate Laboratory i4HB—Institute for Health and Bioeconomy, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, PortugalMARE-Marine and Environmental Sciences Centre, ESTM, Polytechnic of Leiria, 2520-630 Peniche, PortugalMARE-Marine and Environmental Sciences Centre, ESTM, Polytechnic of Leiria, 2520-630 Peniche, PortugalThe increase of antimicrobial resistant strains is leading to an emerging threat to public health. Pathogenic <i>Vibrio</i> are responsible for human and animal illness. The Enterobacteriaceae family includes microorganisms that affect humans, causing several infections. One of the main causes of human infection is related to the ingestion of undercooked seafood. Due to their filter-feeding habit, marine invertebrates, such as clams, are known to be a natural reservoir of specific microbial communities. In the present study, Vibrionaceae and coliforms microorganisms were isolated from clams. A microbial susceptibility test was performed using the disk diffusion method. From 43 presumptive <i>Vibrio</i> spp. and 17 coliforms, three <i>Vibrio</i> spp. with MICs to colistin >512 mg L<sup>−1</sup> were found. From the 23 antimicrobial resistance genes investigated, only the three isolates that showed phenotypic resistance to colistin contained the <i>mcr-1</i> gene. Genotypic analysis for virulence genes in EB07V indicated <i>chiA</i> gene presence. The results from the plasmid cure and transformation showed that the resistance is chromosomally mediated. Biochemical analysis and MLSA, on the basis of four protein-coding gene sequences (<i>recA, rpoB</i>, <i>groEL</i> and <i>dnaJ</i>), grouped the isolates into the genus <i>Vibrio</i> but distinguished them as different from any known <i>Vibrio</i> spp.https://www.mdpi.com/2076-2607/10/2/394antimicrobial resistancecolistin<i>mcr-1</i><i>Vibrio</i> spp. |
| spellingShingle | Christian Valdez Cátia Costa Marco Simões Carla C. C. R. de Carvalho Teresa Baptista Maria J. Campos Detection of <i>mcr-1</i> Gene in Undefined <i>Vibrio</i> Species Isolated from Clams Microorganisms antimicrobial resistance colistin <i>mcr-1</i> <i>Vibrio</i> spp. |
| title | Detection of <i>mcr-1</i> Gene in Undefined <i>Vibrio</i> Species Isolated from Clams |
| title_full | Detection of <i>mcr-1</i> Gene in Undefined <i>Vibrio</i> Species Isolated from Clams |
| title_fullStr | Detection of <i>mcr-1</i> Gene in Undefined <i>Vibrio</i> Species Isolated from Clams |
| title_full_unstemmed | Detection of <i>mcr-1</i> Gene in Undefined <i>Vibrio</i> Species Isolated from Clams |
| title_short | Detection of <i>mcr-1</i> Gene in Undefined <i>Vibrio</i> Species Isolated from Clams |
| title_sort | detection of i mcr 1 i gene in undefined i vibrio i species isolated from clams |
| topic | antimicrobial resistance colistin <i>mcr-1</i> <i>Vibrio</i> spp. |
| url | https://www.mdpi.com/2076-2607/10/2/394 |
| work_keys_str_mv | AT christianvaldez detectionofimcr1igeneinundefinedivibrioispeciesisolatedfromclams AT catiacosta detectionofimcr1igeneinundefinedivibrioispeciesisolatedfromclams AT marcosimoes detectionofimcr1igeneinundefinedivibrioispeciesisolatedfromclams AT carlaccrdecarvalho detectionofimcr1igeneinundefinedivibrioispeciesisolatedfromclams AT teresabaptista detectionofimcr1igeneinundefinedivibrioispeciesisolatedfromclams AT mariajcampos detectionofimcr1igeneinundefinedivibrioispeciesisolatedfromclams |