Effects of Particle Geometry for PLGA-Based Nanoparticles: Preparation and In Vitro/In Vivo Evaluation
The physicochemical properties (size, shape, zeta potential, porosity, elasticity, etc.) of nanocarriers influence their biological behavior directly, which may result in alterations of the therapeutic outcome. Understanding the effect of shape on the cellular interaction and biodistribution of intr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/1/175 |
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| author | Meryem Kaplan Kıvılcım Öztürk Süleyman Can Öztürk Ece Tavukçuoğlu Güneş Esendağlı Sema Calis |
| author_facet | Meryem Kaplan Kıvılcım Öztürk Süleyman Can Öztürk Ece Tavukçuoğlu Güneş Esendağlı Sema Calis |
| author_sort | Meryem Kaplan |
| collection | DOAJ |
| description | The physicochemical properties (size, shape, zeta potential, porosity, elasticity, etc.) of nanocarriers influence their biological behavior directly, which may result in alterations of the therapeutic outcome. Understanding the effect of shape on the cellular interaction and biodistribution of intravenously injected particles could have fundamental importance for the rational design of drug delivery systems. In the present study, spherical, rod and elliptical disk-shaped PLGA nanoparticles were developed for examining systematically their behavior in vitro and in vivo. An important finding is that the release of the encapsulated human serum albumin (HSA) was significantly higher in spherical particles compared to rod and elliptical disks, indicating that the shape can make a difference. Safety studies showed that the toxicity of PLGA nanoparticles is not shape dependent in the studied concentration range. This study has pioneering findings on comparing spherical, rod and elliptical disk-shaped PLGA nanoparticles in terms of particle size, particle size distribution, colloidal stability, morphology, drug encapsulation, drug release, safety of nanoparticles, cellular uptake and biodistribution. Nude mice bearing non-small cell lung cancer were treated with 3 differently shaped nanoparticles, and the accumulation of nanoparticles in tumor tissue and other organs was not statistically different (<i>p</i> > 0.05). It was found that PLGA nanoparticles with 1.00, 4.0 ± 0.5, 7.5 ± 0.5 aspect ratios did not differ on total tumor accumulation in non-small cell lung cancer. |
| first_indexed | 2024-03-09T11:27:35Z |
| format | Article |
| id | doaj.art-7370cea167584acea8f38751172e648a |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T11:27:35Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-7370cea167584acea8f38751172e648a2023-11-30T23:58:45ZengMDPI AGPharmaceutics1999-49232023-01-0115117510.3390/pharmaceutics15010175Effects of Particle Geometry for PLGA-Based Nanoparticles: Preparation and In Vitro/In Vivo EvaluationMeryem Kaplan0Kıvılcım Öztürk1Süleyman Can Öztürk2Ece Tavukçuoğlu3Güneş Esendağlı4Sema Calis5Department of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, Ankara 06100, TurkeyDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, Ankara 06100, TurkeyCentre for Laboratory Animals Research and Application, Hacettepe University, Ankara 06100, TurkeyDepartment of Basic Oncology, Hacettepe University Cancer Institute, Ankara 06100, TurkeyDepartment of Basic Oncology, Hacettepe University Cancer Institute, Ankara 06100, TurkeyDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, Ankara 06100, TurkeyThe physicochemical properties (size, shape, zeta potential, porosity, elasticity, etc.) of nanocarriers influence their biological behavior directly, which may result in alterations of the therapeutic outcome. Understanding the effect of shape on the cellular interaction and biodistribution of intravenously injected particles could have fundamental importance for the rational design of drug delivery systems. In the present study, spherical, rod and elliptical disk-shaped PLGA nanoparticles were developed for examining systematically their behavior in vitro and in vivo. An important finding is that the release of the encapsulated human serum albumin (HSA) was significantly higher in spherical particles compared to rod and elliptical disks, indicating that the shape can make a difference. Safety studies showed that the toxicity of PLGA nanoparticles is not shape dependent in the studied concentration range. This study has pioneering findings on comparing spherical, rod and elliptical disk-shaped PLGA nanoparticles in terms of particle size, particle size distribution, colloidal stability, morphology, drug encapsulation, drug release, safety of nanoparticles, cellular uptake and biodistribution. Nude mice bearing non-small cell lung cancer were treated with 3 differently shaped nanoparticles, and the accumulation of nanoparticles in tumor tissue and other organs was not statistically different (<i>p</i> > 0.05). It was found that PLGA nanoparticles with 1.00, 4.0 ± 0.5, 7.5 ± 0.5 aspect ratios did not differ on total tumor accumulation in non-small cell lung cancer.https://www.mdpi.com/1999-4923/15/1/175nanoparticlesparticle shapeanisotrophuman serum albumincellular uptakebiodistribution |
| spellingShingle | Meryem Kaplan Kıvılcım Öztürk Süleyman Can Öztürk Ece Tavukçuoğlu Güneş Esendağlı Sema Calis Effects of Particle Geometry for PLGA-Based Nanoparticles: Preparation and In Vitro/In Vivo Evaluation Pharmaceutics nanoparticles particle shape anisotrop human serum albumin cellular uptake biodistribution |
| title | Effects of Particle Geometry for PLGA-Based Nanoparticles: Preparation and In Vitro/In Vivo Evaluation |
| title_full | Effects of Particle Geometry for PLGA-Based Nanoparticles: Preparation and In Vitro/In Vivo Evaluation |
| title_fullStr | Effects of Particle Geometry for PLGA-Based Nanoparticles: Preparation and In Vitro/In Vivo Evaluation |
| title_full_unstemmed | Effects of Particle Geometry for PLGA-Based Nanoparticles: Preparation and In Vitro/In Vivo Evaluation |
| title_short | Effects of Particle Geometry for PLGA-Based Nanoparticles: Preparation and In Vitro/In Vivo Evaluation |
| title_sort | effects of particle geometry for plga based nanoparticles preparation and in vitro in vivo evaluation |
| topic | nanoparticles particle shape anisotrop human serum albumin cellular uptake biodistribution |
| url | https://www.mdpi.com/1999-4923/15/1/175 |
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