A myxobacterial GH19 lysozyme with bacteriolytic activity on both Gram-positive and negative phytopathogens
Abstract Myxobacteria, as predatory bacteria, have good application potential in the biocontrol of pathogenic microorganisms. Extracellular enzymes are thought to play an important role in their predation and also provide resources for discovering new antibacterial molecules. We previously isolated...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2022-05-01
|
| Series: | AMB Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13568-022-01393-y |
| _version_ | 1818210271349440512 |
|---|---|
| author | Yueqiu Li Xiaoli Zhou Xianjiao Zhang Zhiqiang Xu Honghong Dong Guohui Yu Ping Cheng Qing Yao Honghui Zhu |
| author_facet | Yueqiu Li Xiaoli Zhou Xianjiao Zhang Zhiqiang Xu Honghong Dong Guohui Yu Ping Cheng Qing Yao Honghui Zhu |
| author_sort | Yueqiu Li |
| collection | DOAJ |
| description | Abstract Myxobacteria, as predatory bacteria, have good application potential in the biocontrol of pathogenic microorganisms. Extracellular enzymes are thought to play an important role in their predation and also provide resources for discovering new antibacterial molecules. We previously isolated a myxobacterium, Corallococcus silvisoli c25j21 GDMCC 1.1387, which is predatory to plant pathogenic bacteria. In this study, we identified an endolysin-like GH19 glycoside hydrolase, C25GH19B, from the genome of c25j21. After its heterologous expression and purification from E. coli, the enzymatic properties of C25GH19B were characterized. C25GH19B showed lysozyme activity with the optimal reaction conditions at 40 °C and pH 4.5–5.0. Moreover, C25GH19B showed bacteriolytic activity against both Gram-positive and Gram-negative plant pathogenic bacteria. Our research provides not only a candidate enzyme for the development of novel biocontrol agents but also an experimental basis for further study on the function and mechanisms of extracellular enzymes in myxobacterial predation. |
| first_indexed | 2024-12-12T05:13:57Z |
| format | Article |
| id | doaj.art-7372eb3d2dcd4c1dbb071c122c2d3616 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-12T05:13:57Z |
| publishDate | 2022-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-7372eb3d2dcd4c1dbb071c122c2d36162022-12-22T00:36:49ZengSpringerOpenAMB Express2191-08552022-05-0112111010.1186/s13568-022-01393-yA myxobacterial GH19 lysozyme with bacteriolytic activity on both Gram-positive and negative phytopathogensYueqiu Li0Xiaoli Zhou1Xianjiao Zhang2Zhiqiang Xu3Honghong Dong4Guohui Yu5Ping Cheng6Qing Yao7Honghui Zhu8Key Laboratory of Agricultural Microbiomics and Precision Application-Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of SciencesKey Laboratory of Agricultural Microbiomics and Precision Application-Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of SciencesKey Laboratory of Agricultural Microbiomics and Precision Application-Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of SciencesKey Laboratory of Agricultural Microbiomics and Precision Application-Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of SciencesKey Laboratory of Agricultural Microbiomics and Precision Application-Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of SciencesKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Guangdong University Key Laboratory for Sustainable Control of Fruit and Vegetable Diseases and Pests, Innovative Institute for Plant Health, College of Agriculture and Biology, Zhongkai University of Agriculture and EngineeringKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Guangdong University Key Laboratory for Sustainable Control of Fruit and Vegetable Diseases and Pests, Innovative Institute for Plant Health, College of Agriculture and Biology, Zhongkai University of Agriculture and EngineeringCenter for Litchi, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangdong Engineering Research Center for Grass Science, Guangdong Engineering, College of Horticulture, South China Agricultural UniversityKey Laboratory of Agricultural Microbiomics and Precision Application-Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of SciencesAbstract Myxobacteria, as predatory bacteria, have good application potential in the biocontrol of pathogenic microorganisms. Extracellular enzymes are thought to play an important role in their predation and also provide resources for discovering new antibacterial molecules. We previously isolated a myxobacterium, Corallococcus silvisoli c25j21 GDMCC 1.1387, which is predatory to plant pathogenic bacteria. In this study, we identified an endolysin-like GH19 glycoside hydrolase, C25GH19B, from the genome of c25j21. After its heterologous expression and purification from E. coli, the enzymatic properties of C25GH19B were characterized. C25GH19B showed lysozyme activity with the optimal reaction conditions at 40 °C and pH 4.5–5.0. Moreover, C25GH19B showed bacteriolytic activity against both Gram-positive and Gram-negative plant pathogenic bacteria. Our research provides not only a candidate enzyme for the development of novel biocontrol agents but also an experimental basis for further study on the function and mechanisms of extracellular enzymes in myxobacterial predation.https://doi.org/10.1186/s13568-022-01393-yMyxobacteriaLysozymeBacteriolytic activityPhytopathogens |
| spellingShingle | Yueqiu Li Xiaoli Zhou Xianjiao Zhang Zhiqiang Xu Honghong Dong Guohui Yu Ping Cheng Qing Yao Honghui Zhu A myxobacterial GH19 lysozyme with bacteriolytic activity on both Gram-positive and negative phytopathogens AMB Express Myxobacteria Lysozyme Bacteriolytic activity Phytopathogens |
| title | A myxobacterial GH19 lysozyme with bacteriolytic activity on both Gram-positive and negative phytopathogens |
| title_full | A myxobacterial GH19 lysozyme with bacteriolytic activity on both Gram-positive and negative phytopathogens |
| title_fullStr | A myxobacterial GH19 lysozyme with bacteriolytic activity on both Gram-positive and negative phytopathogens |
| title_full_unstemmed | A myxobacterial GH19 lysozyme with bacteriolytic activity on both Gram-positive and negative phytopathogens |
| title_short | A myxobacterial GH19 lysozyme with bacteriolytic activity on both Gram-positive and negative phytopathogens |
| title_sort | myxobacterial gh19 lysozyme with bacteriolytic activity on both gram positive and negative phytopathogens |
| topic | Myxobacteria Lysozyme Bacteriolytic activity Phytopathogens |
| url | https://doi.org/10.1186/s13568-022-01393-y |
| work_keys_str_mv | AT yueqiuli amyxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT xiaolizhou amyxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT xianjiaozhang amyxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT zhiqiangxu amyxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT honghongdong amyxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT guohuiyu amyxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT pingcheng amyxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT qingyao amyxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT honghuizhu amyxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT yueqiuli myxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT xiaolizhou myxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT xianjiaozhang myxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT zhiqiangxu myxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT honghongdong myxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT guohuiyu myxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT pingcheng myxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT qingyao myxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens AT honghuizhu myxobacterialgh19lysozymewithbacteriolyticactivityonbothgrampositiveandnegativephytopathogens |