The Viability of the Lactobacillus Rhamnosus IL4.2 Strain in Simulated Gastrointestinal Conditions
The viability maintenance of Lactobacillus rhamnosus IL4.2 strain in gastrointestinal conditions represents one of the most important characteristics regarding its use for obtaining probiotic products. The tests were performed with a cell suspension kept in 0.5% NaCl. The influence of pepsin (3 g/l)...
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Agroprint Timisoara
2023-11-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/1802 |
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| author | Emanuel Vamanu Adrian Vamanu Diana Pelinescu Sultana Nita Nicoleta Rusu |
| author_facet | Emanuel Vamanu Adrian Vamanu Diana Pelinescu Sultana Nita Nicoleta Rusu |
| author_sort | Emanuel Vamanu |
| collection | DOAJ |
| description | The viability maintenance of Lactobacillus rhamnosus IL4.2 strain in gastrointestinal conditions represents one of the most important characteristics regarding its use for obtaining probiotic products. The tests were performed with a cell suspension kept in 0.5% NaCl. The influence of pepsin (3 g/l) at pH of 1.5, 2, 2.5 and 3, as well as of pancreatin (1 g/l) in the presence of bile salts (1.5, 2, 3 and 5 mg/ml) were determined. The influence of casein and mucin, in a concentration of 1 g/l, was also established in the aforementioned conditions. It was observed that mucin presented a longer viability maintenance, fact also confirmed by the calculation of the mathematical parameters of viability and mortality, when mucin was either used or not, especially in the case of gastric transit. The results proved that the tested strain maintained its viability even at pH between 1.8 - 2 and at an even higher concentration, of 2 mg/ml of bile salts, but up to two hours as of the exposure to the conditions of the simulated small intestinal juice. Such results were also confirmed by the cumulated effect of the simulated gastric and small intestinal juice, the strain thus increasing its viability with an average of 10% in the presence of mucin. |
| first_indexed | 2024-04-24T11:49:09Z |
| format | Article |
| id | doaj.art-7375f60d67644550ac5147b8e637b54b |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:49:09Z |
| publishDate | 2023-11-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-7375f60d67644550ac5147b8e637b54b2024-04-09T08:38:11ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-11-014414594591802The Viability of the Lactobacillus Rhamnosus IL4.2 Strain in Simulated Gastrointestinal ConditionsEmanuel Vamanu0Adrian Vamanu1Diana Pelinescu2Sultana Nita3Nicoleta Rusu4University of Agronomic Sciences and Veterinary Medicine-Faculty of Biotechnology & Biotehnol Center Bucharest, Bd. Mărăşti no. 59, district 1, Bucharest, RomaniaUniversity of Agronomic Sciences and Veterinary Medicine-Faculty of Biotechnology & Biotehnol Center Bucharest, Bd. Mărăşti no. 59, district 1, Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, Splaiul Independenţei no. 91-95, district 5, zip 76201, Bucharest, RomaniaNational Institute of Chemical-Pharmaceutical Research-Development – ICCF Bucharest, Vitan Road no. 112, sector 3, Bucharest, RomaniaNational Institute of Chemical-Pharmaceutical Research-Development – ICCF Bucharest, Vitan Road no. 112, sector 3, Bucharest, RomaniaThe viability maintenance of Lactobacillus rhamnosus IL4.2 strain in gastrointestinal conditions represents one of the most important characteristics regarding its use for obtaining probiotic products. The tests were performed with a cell suspension kept in 0.5% NaCl. The influence of pepsin (3 g/l) at pH of 1.5, 2, 2.5 and 3, as well as of pancreatin (1 g/l) in the presence of bile salts (1.5, 2, 3 and 5 mg/ml) were determined. The influence of casein and mucin, in a concentration of 1 g/l, was also established in the aforementioned conditions. It was observed that mucin presented a longer viability maintenance, fact also confirmed by the calculation of the mathematical parameters of viability and mortality, when mucin was either used or not, especially in the case of gastric transit. The results proved that the tested strain maintained its viability even at pH between 1.8 - 2 and at an even higher concentration, of 2 mg/ml of bile salts, but up to two hours as of the exposure to the conditions of the simulated small intestinal juice. Such results were also confirmed by the cumulated effect of the simulated gastric and small intestinal juice, the strain thus increasing its viability with an average of 10% in the presence of mucin.https://spasb.ro/index.php/public_html/article/view/1802caseincolonyquantmucinpancreatinpepsin |
| spellingShingle | Emanuel Vamanu Adrian Vamanu Diana Pelinescu Sultana Nita Nicoleta Rusu The Viability of the Lactobacillus Rhamnosus IL4.2 Strain in Simulated Gastrointestinal Conditions Scientific Papers Animal Science and Biotechnologies casein colonyquant mucin pancreatin pepsin |
| title | The Viability of the Lactobacillus Rhamnosus IL4.2 Strain in Simulated Gastrointestinal Conditions |
| title_full | The Viability of the Lactobacillus Rhamnosus IL4.2 Strain in Simulated Gastrointestinal Conditions |
| title_fullStr | The Viability of the Lactobacillus Rhamnosus IL4.2 Strain in Simulated Gastrointestinal Conditions |
| title_full_unstemmed | The Viability of the Lactobacillus Rhamnosus IL4.2 Strain in Simulated Gastrointestinal Conditions |
| title_short | The Viability of the Lactobacillus Rhamnosus IL4.2 Strain in Simulated Gastrointestinal Conditions |
| title_sort | viability of the lactobacillus rhamnosus il4 2 strain in simulated gastrointestinal conditions |
| topic | casein colonyquant mucin pancreatin pepsin |
| url | https://spasb.ro/index.php/public_html/article/view/1802 |
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