Meiotic Spindle Formation Following Inhibition of First Polar Body Formation in the Zhikong Scallop (Chlamys farreri Jones et Preston)
Fertilized Zhikong scallop (Chlamys farreri) eggs were treated with cytochalasin B (CB 0.5 mg/L) at 14–15 min postfertilization to inhibit first polar body formation. The eggs were then stained with fluorescein isothiocyanate (FITC) -anti-α-tubulin and propidium iodide (PI) to examine their microtub...
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2021.653000/full |
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| author | Yongren Li Baolu Zhang Shuang Liang Yongjun Guo |
| author_facet | Yongren Li Baolu Zhang Shuang Liang Yongjun Guo |
| author_sort | Yongren Li |
| collection | DOAJ |
| description | Fertilized Zhikong scallop (Chlamys farreri) eggs were treated with cytochalasin B (CB 0.5 mg/L) at 14–15 min postfertilization to inhibit first polar body formation. The eggs were then stained with fluorescein isothiocyanate (FITC) -anti-α-tubulin and propidium iodide (PI) to examine their microtubule patterns and chromosome, respectively. Fluorescent microscope observations of treated eggs sampled every 2–3 min during meiotic maturation revealed meiotic apparatus assembly and correlated chromosome segregation. In CB-treated groups, meiosis I proceeded normally and produced two groups of dyads, with 19 in each group. Both dyad groups were retained in the eggs as they entered meiosis II. Two, three, or four asters (centrosome with microtubules around it) in meiosis II rearranged the spindle in several patterns: bipolar [24.0 ± 4.1 μm (long axis) × 18.3 ± 4.1 μm (diameter: metaphase plate)], tripolar (18.6 ± 3.9 μm × 9.9 ± 1.3 μm), separated bipolar (18.3 ± 2.8 μm × 11.2 ± 1.8 μm), and other unclassified spindle patterns. Corresponding chromosome segregation, including bipolar (18.9%), tripolar (38.9%), double bipolar (16.5%), and unclassified (25.6%), was observed during meiosis II in CB-treated eggs. The data indicated that chromosome segregation patterns determined by spindle patterns were critically influenced by the number of centrosomes in meiosis II eggs following inhibition of polar body 1 (PB1) formation with CB. |
| first_indexed | 2024-12-16T17:02:07Z |
| format | Article |
| id | doaj.art-737b095103a74a5ba014f8599114c702 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-16T17:02:07Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-737b095103a74a5ba014f8599114c7022022-12-21T22:23:42ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452021-03-01810.3389/fmars.2021.653000653000Meiotic Spindle Formation Following Inhibition of First Polar Body Formation in the Zhikong Scallop (Chlamys farreri Jones et Preston)Yongren Li0Baolu Zhang1Shuang Liang2Yongjun Guo3Key Laboratory for Aquatic Ecology and Aquaculture of Tianjin, Department of Fisheries Science, Tianjin Agricultural University, Tianjin, ChinaOceanic Consultation Center, Ministry of Natural Resources of the People’s Republic of China, Beijing, ChinaKey Laboratory for Aquatic Ecology and Aquaculture of Tianjin, Department of Fisheries Science, Tianjin Agricultural University, Tianjin, ChinaKey Laboratory for Aquatic Ecology and Aquaculture of Tianjin, Department of Fisheries Science, Tianjin Agricultural University, Tianjin, ChinaFertilized Zhikong scallop (Chlamys farreri) eggs were treated with cytochalasin B (CB 0.5 mg/L) at 14–15 min postfertilization to inhibit first polar body formation. The eggs were then stained with fluorescein isothiocyanate (FITC) -anti-α-tubulin and propidium iodide (PI) to examine their microtubule patterns and chromosome, respectively. Fluorescent microscope observations of treated eggs sampled every 2–3 min during meiotic maturation revealed meiotic apparatus assembly and correlated chromosome segregation. In CB-treated groups, meiosis I proceeded normally and produced two groups of dyads, with 19 in each group. Both dyad groups were retained in the eggs as they entered meiosis II. Two, three, or four asters (centrosome with microtubules around it) in meiosis II rearranged the spindle in several patterns: bipolar [24.0 ± 4.1 μm (long axis) × 18.3 ± 4.1 μm (diameter: metaphase plate)], tripolar (18.6 ± 3.9 μm × 9.9 ± 1.3 μm), separated bipolar (18.3 ± 2.8 μm × 11.2 ± 1.8 μm), and other unclassified spindle patterns. Corresponding chromosome segregation, including bipolar (18.9%), tripolar (38.9%), double bipolar (16.5%), and unclassified (25.6%), was observed during meiosis II in CB-treated eggs. The data indicated that chromosome segregation patterns determined by spindle patterns were critically influenced by the number of centrosomes in meiosis II eggs following inhibition of polar body 1 (PB1) formation with CB.https://www.frontiersin.org/articles/10.3389/fmars.2021.653000/fullChlamys farreripolyploidmeiotic spindlechromosome segregationcytochalasin B |
| spellingShingle | Yongren Li Baolu Zhang Shuang Liang Yongjun Guo Meiotic Spindle Formation Following Inhibition of First Polar Body Formation in the Zhikong Scallop (Chlamys farreri Jones et Preston) Frontiers in Marine Science Chlamys farreri polyploid meiotic spindle chromosome segregation cytochalasin B |
| title | Meiotic Spindle Formation Following Inhibition of First Polar Body Formation in the Zhikong Scallop (Chlamys farreri Jones et Preston) |
| title_full | Meiotic Spindle Formation Following Inhibition of First Polar Body Formation in the Zhikong Scallop (Chlamys farreri Jones et Preston) |
| title_fullStr | Meiotic Spindle Formation Following Inhibition of First Polar Body Formation in the Zhikong Scallop (Chlamys farreri Jones et Preston) |
| title_full_unstemmed | Meiotic Spindle Formation Following Inhibition of First Polar Body Formation in the Zhikong Scallop (Chlamys farreri Jones et Preston) |
| title_short | Meiotic Spindle Formation Following Inhibition of First Polar Body Formation in the Zhikong Scallop (Chlamys farreri Jones et Preston) |
| title_sort | meiotic spindle formation following inhibition of first polar body formation in the zhikong scallop chlamys farreri jones et preston |
| topic | Chlamys farreri polyploid meiotic spindle chromosome segregation cytochalasin B |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2021.653000/full |
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