<i>Let-7</i> miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma
The <i>lethal-7 (let-7)</i> family is an important microRNA (miRNA) group that usually exerts functions as a tumor suppressor. We aimed to evaluate the expression profile of <i>let-7a, let-7b, let-7c, let-7d, let-7e, let-7f, let-7g</i>, and <i>let-7i</i> and to as...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/11/1452 |
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| author | Bruna Cristine de Almeida Laura Gonzalez dos Anjos Miyuki Uno Isabela Werneck da Cunha Fernando Augusto Soares Glauco Baiocchi Edmund Chada Baracat Katia Candido Carvalho |
| author_facet | Bruna Cristine de Almeida Laura Gonzalez dos Anjos Miyuki Uno Isabela Werneck da Cunha Fernando Augusto Soares Glauco Baiocchi Edmund Chada Baracat Katia Candido Carvalho |
| author_sort | Bruna Cristine de Almeida |
| collection | DOAJ |
| description | The <i>lethal-7 (let-7)</i> family is an important microRNA (miRNA) group that usually exerts functions as a tumor suppressor. We aimed to evaluate the expression profile of <i>let-7a, let-7b, let-7c, let-7d, let-7e, let-7f, let-7g</i>, and <i>let-7i</i> and to assess their value as prognostic markers in uterine leiomyosarcoma (LMS) patients. The miRNAs expression profile was assessed in 34 LMS and 13 normal myometrium (MM) paraffin-embedded samples. All <i>let-7</i> family members showed downregulation in LMS. Our findings showed that patients with <i>let-7e</i> downregulation had worse overall survival (OS) and is an independent prognostic factor (hazard ratio [HR] = 2.24). In addition, almost half the patients had distant metastasis. LMS patients with downregulated <i>let-7b</i> and <i>let-7d</i> had worse disease-free survival (DFS); they are not independent prognostic factors (HR = 2.65). Patients’ ages were associated with <i>let-7d, let-7e</i> and <i>let-7f</i> (<i>p</i> = 0.0160) downregulation. In conclusion, all the <i>let-7</i> family members were downregulated in LMS patients, and the greater the loss of expression of these molecules, the greater their relationship with worse prognosis of patients. <i>Let-7e</i> expression might influence the OS, while <i>let-7b</i> and <i>le-7d</i> might influence the DFS. The lowest expression levels of <i>let-7d, let-7e</i>, and <i>let-7f</i> were associated with the oldest patients. Our findings indicate strong evidence of <i>let-7’s</i> role as a potential prognostic biomarker in LMS. |
| first_indexed | 2024-03-12T06:30:06Z |
| format | Article |
| id | doaj.art-737c8100768548f288d815dc73f44cb2 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T06:30:06Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-737c8100768548f288d815dc73f44cb22023-09-03T01:41:05ZengMDPI AGCells2073-44092019-11-01811145210.3390/cells8111452cells8111452<i>Let-7</i> miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine LeiomyosarcomaBruna Cristine de Almeida0Laura Gonzalez dos Anjos1Miyuki Uno2Isabela Werneck da Cunha3Fernando Augusto Soares4Glauco Baiocchi5Edmund Chada Baracat6Katia Candido Carvalho7Laboratório de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, Cerqueira Cesar, São Paulo 05403-010, BrazilLaboratório de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, Cerqueira Cesar, São Paulo 05403-010, BrazilCentro de Investigação Translacional em Oncologia (LIM 24), Instituto do Câncer do Estado de São Paulo (CTO/ICESP) Av Dr Arnaldo 251 sala 23 8 andar, São Paulo 01246000, BrazilDepartment of Pathology, Rede D’OR-São Luiz, Rua das Perobas, 344-Jabaquara, São Paulo 04321-120, BrazilDepartment of Pathology, Rede D’OR-São Luiz, Rua das Perobas, 344-Jabaquara, São Paulo 04321-120, BrazilDepartment of Gynecology Oncology, A.C.Camargo Cancer Center, Rua Prof Antonio Prudente 211, São Paulo 01509-001, BrazilLaboratório de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, Cerqueira Cesar, São Paulo 05403-010, BrazilLaboratório de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, Cerqueira Cesar, São Paulo 05403-010, BrazilThe <i>lethal-7 (let-7)</i> family is an important microRNA (miRNA) group that usually exerts functions as a tumor suppressor. We aimed to evaluate the expression profile of <i>let-7a, let-7b, let-7c, let-7d, let-7e, let-7f, let-7g</i>, and <i>let-7i</i> and to assess their value as prognostic markers in uterine leiomyosarcoma (LMS) patients. The miRNAs expression profile was assessed in 34 LMS and 13 normal myometrium (MM) paraffin-embedded samples. All <i>let-7</i> family members showed downregulation in LMS. Our findings showed that patients with <i>let-7e</i> downregulation had worse overall survival (OS) and is an independent prognostic factor (hazard ratio [HR] = 2.24). In addition, almost half the patients had distant metastasis. LMS patients with downregulated <i>let-7b</i> and <i>let-7d</i> had worse disease-free survival (DFS); they are not independent prognostic factors (HR = 2.65). Patients’ ages were associated with <i>let-7d, let-7e</i> and <i>let-7f</i> (<i>p</i> = 0.0160) downregulation. In conclusion, all the <i>let-7</i> family members were downregulated in LMS patients, and the greater the loss of expression of these molecules, the greater their relationship with worse prognosis of patients. <i>Let-7e</i> expression might influence the OS, while <i>let-7b</i> and <i>le-7d</i> might influence the DFS. The lowest expression levels of <i>let-7d, let-7e</i>, and <i>let-7f</i> were associated with the oldest patients. Our findings indicate strong evidence of <i>let-7’s</i> role as a potential prognostic biomarker in LMS.https://www.mdpi.com/2073-4409/8/11/1452<i>let-7</i> familymirnauterine leiomyosarcomanon-coding rna |
| spellingShingle | Bruna Cristine de Almeida Laura Gonzalez dos Anjos Miyuki Uno Isabela Werneck da Cunha Fernando Augusto Soares Glauco Baiocchi Edmund Chada Baracat Katia Candido Carvalho <i>Let-7</i> miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma Cells <i>let-7</i> family mirna uterine leiomyosarcoma non-coding rna |
| title | <i>Let-7</i> miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma |
| title_full | <i>Let-7</i> miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma |
| title_fullStr | <i>Let-7</i> miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma |
| title_full_unstemmed | <i>Let-7</i> miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma |
| title_short | <i>Let-7</i> miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma |
| title_sort | i let 7 i mirna s expression profile and its potential prognostic role in uterine leiomyosarcoma |
| topic | <i>let-7</i> family mirna uterine leiomyosarcoma non-coding rna |
| url | https://www.mdpi.com/2073-4409/8/11/1452 |
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