Generating a mouse model for relapsed Sonic Hedgehog medulloblastoma
Summary: Tumor relapse is the leading adverse prognostic factor in medulloblastoma (MB). However, there is still no established mouse model for MB relapse, impeding our efforts to develop strategies to treat relapsed MB. We present a protocol for generating a mouse model for relapsed MB using irradi...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723001922 |
| _version_ | 1827964692496121856 |
|---|---|
| author | Allie Heller Fang Du Yongqiang Liu Yijun Yang Zeng-Jie Yang |
| author_facet | Allie Heller Fang Du Yongqiang Liu Yijun Yang Zeng-Jie Yang |
| author_sort | Allie Heller |
| collection | DOAJ |
| description | Summary: Tumor relapse is the leading adverse prognostic factor in medulloblastoma (MB). However, there is still no established mouse model for MB relapse, impeding our efforts to develop strategies to treat relapsed MB. We present a protocol for generating a mouse model for relapsed MB using irradiation by optimizing mouse breeding and age, as well as irradiation dosage and timing. We then detail procedures for determining tumor relapse based on tumor cell trans-differentiation in MB tissue, immunohistochemistry, and tumor cell isolation.For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-09T17:20:32Z |
| format | Article |
| id | doaj.art-73805969727f4f459c008200d0689074 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-09T17:20:32Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-73805969727f4f459c008200d06890742023-04-19T04:23:20ZengElsevierSTAR Protocols2666-16672023-06-0142102234Generating a mouse model for relapsed Sonic Hedgehog medulloblastomaAllie Heller0Fang Du1Yongqiang Liu2Yijun Yang3Zeng-Jie Yang4Nuclear Dynamics and Cancer Program, Fox Chase Cancer Center, Temple University Health System, Philadelphia, PA 19111, USANuclear Dynamics and Cancer Program, Fox Chase Cancer Center, Temple University Health System, Philadelphia, PA 19111, USANuclear Dynamics and Cancer Program, Fox Chase Cancer Center, Temple University Health System, Philadelphia, PA 19111, USANuclear Dynamics and Cancer Program, Fox Chase Cancer Center, Temple University Health System, Philadelphia, PA 19111, USA; Corresponding authorNuclear Dynamics and Cancer Program, Fox Chase Cancer Center, Temple University Health System, Philadelphia, PA 19111, USA; Corresponding authorSummary: Tumor relapse is the leading adverse prognostic factor in medulloblastoma (MB). However, there is still no established mouse model for MB relapse, impeding our efforts to develop strategies to treat relapsed MB. We present a protocol for generating a mouse model for relapsed MB using irradiation by optimizing mouse breeding and age, as well as irradiation dosage and timing. We then detail procedures for determining tumor relapse based on tumor cell trans-differentiation in MB tissue, immunohistochemistry, and tumor cell isolation.For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723001922Cell IsolationCancerMicroscopyModel OrganismsNeuroscience |
| spellingShingle | Allie Heller Fang Du Yongqiang Liu Yijun Yang Zeng-Jie Yang Generating a mouse model for relapsed Sonic Hedgehog medulloblastoma STAR Protocols Cell Isolation Cancer Microscopy Model Organisms Neuroscience |
| title | Generating a mouse model for relapsed Sonic Hedgehog medulloblastoma |
| title_full | Generating a mouse model for relapsed Sonic Hedgehog medulloblastoma |
| title_fullStr | Generating a mouse model for relapsed Sonic Hedgehog medulloblastoma |
| title_full_unstemmed | Generating a mouse model for relapsed Sonic Hedgehog medulloblastoma |
| title_short | Generating a mouse model for relapsed Sonic Hedgehog medulloblastoma |
| title_sort | generating a mouse model for relapsed sonic hedgehog medulloblastoma |
| topic | Cell Isolation Cancer Microscopy Model Organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723001922 |
| work_keys_str_mv | AT allieheller generatingamousemodelforrelapsedsonichedgehogmedulloblastoma AT fangdu generatingamousemodelforrelapsedsonichedgehogmedulloblastoma AT yongqiangliu generatingamousemodelforrelapsedsonichedgehogmedulloblastoma AT yijunyang generatingamousemodelforrelapsedsonichedgehogmedulloblastoma AT zengjieyang generatingamousemodelforrelapsedsonichedgehogmedulloblastoma |