Re-introducing non-optimal synonymous codons into codon-optimized constructs enhances soluble recovery of recombinant proteins from Escherichia coli.
Gene synthesis services have largely superseded traditional PCR methods for the generation of cDNAs destined for bacterial expression vectors. This, in turn, has increased the application of codon-optimized cDNAs where codons rarely used by Escherchia coli are replaced with common synonymous codons...
Main Authors: | Jennifer Konczal, Justin Bower, Christopher H Gray |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2019-01-01
|
Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0215892 |
Similar Items
-
Soluble expression and catalytic properties of codon-optimized recombinant bromelain from MD2 pineapple in Escherichia coli
by: Rafida Razali, et al.
Published: (2021) -
Relative synonymous codon usage and codon pair analysis of depression associated genes
by: Rekha Khandia, et al.
Published: (2024-02-01) -
Emergent rules for codon choice elucidated by editing rare arginine codons in Escherichia coli
by: Napolitano, Michael G., et al.
Published: (2017) -
Synonymous codon usage regulates translation initiation
by: Chloe L. Barrington, et al.
Published: (2023-12-01) -
Pan-cancer analyses of synonymous mutations based on tissue-specific codon optimality
by: Xia Ran, et al.
Published: (2022-01-01)