Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA
The Old Kladruber horse is the oldest original Czech horse breed - gene resource, with pedigree records spanning three centuries. Because the population is closed, there is a concern about the loss of genetic variation. The genetic diversity within and amongst sire lines was evaluated using microsat...
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Czech Academy of Agricultural Sciences
2011-04-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201104-0003_intra-line-and-inter-line-genetic-diversity-in-sire-lines-of-the-old-kladruber-horse-based-on-microsatellite-an.php |
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| author | L. Vostrý O. Kracíková B. Hofmanová V. Czerneková T. Kott J. Přibyl |
| author_facet | L. Vostrý O. Kracíková B. Hofmanová V. Czerneková T. Kott J. Přibyl |
| author_sort | L. Vostrý |
| collection | DOAJ |
| description | The Old Kladruber horse is the oldest original Czech horse breed - gene resource, with pedigree records spanning three centuries. Because the population is closed, there is a concern about the loss of genetic variation. The genetic diversity within and amongst sire lines was evaluated using microsatellite markers and based on pedigree information. The DNA analysis, covering 16 microsatellite loci, revealed that the genetic diversity (observed heterozygosity = 0.637, genetic diversity = 0.678 and the mean number of alleles = 8.25) for the Old Kladruber horse is consistent with other populations of horses. Inter-line genetic difference in sire lines reached the mean value (the FST values ranged from 0.020 to 0.017). Differences amongst the sire lines were identified using genetic distances and principal component analysis. One gene cluster comprised black variation sire lines, while the second cluster included the sire lines of the grey variation. In the subjects monitored, the average inbreeding coefficient of 0.076 was estimated on the basis of pedigree information. The highest mean values of the coefficient of relationship were estimated within the colour variations. When comparing the genetic distance and the average coefficient of relationship, differences were found out. These results are useful for the development of breeding strategies which consider classical horse breeding as well as recent achievements of population and conservation genetics. |
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| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:27:21Z |
| publishDate | 2011-04-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-73828d9d1cf746a39b60eed61efe430f2023-02-23T03:32:53ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092011-04-0156416317510.17221/1437-CJAScjs-201104-0003Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNAL. Vostrý0O. Kracíková1B. Hofmanová2V. Czerneková3T. Kott4J. Přibyl5Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Science Prague, Prague, Czech RepublicDepartment of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Science Prague, Prague, Czech RepublicDepartment of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Science Prague, Prague, Czech RepublicInstitute of Animal Science Praha Uhříněves, Prague, Czech RepublicInstitute of Animal Science Praha Uhříněves, Prague, Czech RepublicInstitute of Animal Science Praha Uhříněves, Prague, Czech RepublicThe Old Kladruber horse is the oldest original Czech horse breed - gene resource, with pedigree records spanning three centuries. Because the population is closed, there is a concern about the loss of genetic variation. The genetic diversity within and amongst sire lines was evaluated using microsatellite markers and based on pedigree information. The DNA analysis, covering 16 microsatellite loci, revealed that the genetic diversity (observed heterozygosity = 0.637, genetic diversity = 0.678 and the mean number of alleles = 8.25) for the Old Kladruber horse is consistent with other populations of horses. Inter-line genetic difference in sire lines reached the mean value (the FST values ranged from 0.020 to 0.017). Differences amongst the sire lines were identified using genetic distances and principal component analysis. One gene cluster comprised black variation sire lines, while the second cluster included the sire lines of the grey variation. In the subjects monitored, the average inbreeding coefficient of 0.076 was estimated on the basis of pedigree information. The highest mean values of the coefficient of relationship were estimated within the colour variations. When comparing the genetic distance and the average coefficient of relationship, differences were found out. These results are useful for the development of breeding strategies which consider classical horse breeding as well as recent achievements of population and conservation genetics.https://cjas.agriculturejournals.cz/artkey/cjs-201104-0003_intra-line-and-inter-line-genetic-diversity-in-sire-lines-of-the-old-kladruber-horse-based-on-microsatellite-an.phpgenetic variabilitygenetic structureconservationmicrosatellite dnakladruber horseline |
| spellingShingle | L. Vostrý O. Kracíková B. Hofmanová V. Czerneková T. Kott J. Přibyl Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA Czech Journal of Animal Science genetic variability genetic structure conservation microsatellite dna kladruber horse line |
| title | Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA |
| title_full | Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA |
| title_fullStr | Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA |
| title_full_unstemmed | Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA |
| title_short | Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA |
| title_sort | intra line and inter line genetic diversity in sire lines of the old kladruber horse based on microsatellite analysis of dna |
| topic | genetic variability genetic structure conservation microsatellite dna kladruber horse line |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201104-0003_intra-line-and-inter-line-genetic-diversity-in-sire-lines-of-the-old-kladruber-horse-based-on-microsatellite-an.php |
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