| Summary: | In order to study the effect of green manure return for stabilized spring maize (<i>Zea mays</i> L.) grain yield (GY) we reduced nitrogen fertilizer input by regulation and examined effects on soil nutrients, enzyme activity, and fungal communities. This two-year field experiment was conducted in the North China Plain. The field experiment was undertaken with a split-plot design; the primary plots were winter fallow (WF) and green manure (GM), and the split-plots were five N application rates of 0 (N0), 189 (N189), 216 (N216), 243 (N243), and 270 (N270) kg ha<sup>−1</sup>. The results showed that, spring maize GY under GM treatments (GY<i><sub>GM</sub></i>) were significantly increased by 5.38–11.68% more than WF treatment (GY<i><sub>WF</sub></i>), and GY<i><sub>WF</sub></i> and GY<i><sub>GM</sub></i> significantly increased by 35.9–91.5% and 80.1–135.5% across all N treatments. By linear-platform model analysis, spring maize under GM treatments obtained higher GY, reaching 1270.5–14,312.2 kg ha<sup>−1</sup> with optimized N application rate at 238–265 kg ha<sup>−1</sup>, which resulted in a GY higher than WF (11,820.0 and 13,654.2 kg ha<sup>−1</sup>) and N reduced 11.2% (238 vs. 268 kg ha<sup>−1</sup>). GM treatment significantly increased soil organic carbon by 3.90–12.23% more than WF over all N application rates, and total nitrogen and available nitrogen were significantly increased by 3.79–15.76% and 4.87–17.29%, with total phosphorus and available phosphorus for GM higher than WF by 6.1–13.6% and 9.6–5.3%, respectively. However, there were lesser effects of GM on total potassium and available potassium. Compared to WF, soil catalase, sucrose, urease, and alkaline phosphatase activity were significantly increased by 6.2–16.4%, 5.8–48.1%, 3.3–21.5% and 11.5–82.3%, respectively, over all N application rates under GM over two years. GM increased <i>Zygomycota</i> and <i>Basidiomycota</i> relative abundances significantly, and reduced <i>Thielavia</i>, <i>unclassified fungi</i>, and <i>Podospora</i> relative abundances by 35.35%, 52.92% and 52.77% more than WF treatment, respectively. In summary, due to the GM return into fields, increased soil nutrients were available, which were positively affected by soil enzyme activity and fungal communities, and reduced nutrient requirements, and so the farmers could obtain a spring maize grain yield higher than 14,000 kg ha<sup>−1</sup> with a reduced 11.2% N application rate from 268 kg ha<sup>−1</sup> to 238 kg ha<sup>−1</sup> by sowing winter green manure for a long time period in the North China Plain.
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