The relationship between gut microbiota and neonatal pathologic jaundice: A pilot case-control study
Background and objectiveNeonatal jaundice is a common clinical disease in neonates. Pathologic jaundice is more harmful to neonates. There are a few studies on the biomarkers of pathologic jaundice and the correlation between gut microbiota and clinical indices. Therefore, we aimed to reveal the cha...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1122172/full |
| _version_ | 1797869551190278144 |
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| author | Jia Jia You Jia Jia You Jia Jia You Jun Qiu Gui Nan Li Xiao Ming Peng Ye Ma Chang Ci Zhou Chang Ci Zhou Chang Ci Zhou Si Wei Fang Si Wei Fang Si Wei Fang Rui Wen Huang Zheng Hui Xiao |
| author_facet | Jia Jia You Jia Jia You Jia Jia You Jun Qiu Gui Nan Li Xiao Ming Peng Ye Ma Chang Ci Zhou Chang Ci Zhou Chang Ci Zhou Si Wei Fang Si Wei Fang Si Wei Fang Rui Wen Huang Zheng Hui Xiao |
| author_sort | Jia Jia You |
| collection | DOAJ |
| description | Background and objectiveNeonatal jaundice is a common clinical disease in neonates. Pathologic jaundice is more harmful to neonates. There are a few studies on the biomarkers of pathologic jaundice and the correlation between gut microbiota and clinical indices. Therefore, we aimed to reveal the characteristics of gut microbiota in pathologic jaundice, provide potential biomarkers for the diagnosis of pathologic jaundice, and find the correlation between gut microbiota and clinical indices.MethodsFourteen neonates with physiologic jaundice were recruited into a control group (Group A). Additionally, 14 neonates with pathologic jaundice were recruited into a case group (Group B). The microbial communities were analyzed using 16S rDNA sequencing. LEfSe and the differences in the relative abundance of gut microbiota were used to identify different bacteria among the two groups. The ROC curve was used to assess effective biomarkers for pathologic jaundice. Spearman’s rank-sum correlation coefficient was used to evaluate the correlation between gut microbiota and clinical indices.ResultsThere were no differences in the total richness or diversity of gut microbiota between the two groups. At the phylum and genus levels, compared with the control group, Bacteroidetes (p = 0.002) and Braydrhizobium (p = 0.01) were significantly higher, while Actinobacteria (p = 0.003) and Bidfldobacterium (p = 0.016) were significantly lower in the case group. Bacteroidetes were valuable in differentiating pathologic jaundice from physiologic jaundice by the ROC curve, and the area under the ROC curve (AUC) value was 0.839 [95%CI (0.648–0.995)]. In the case group, Bacteroidetes were negatively associated with total bilirubin (TBIL) (p < 0.05). In the control group, Bacteroidetes were positively associated with TBIL (p < 0.05).ConclusionBacteroidetes could be used as biomarkers to identify pathologic jaundice and Bacteroidetes are positively associated with bilirubin levels. |
| first_indexed | 2024-04-10T00:14:28Z |
| format | Article |
| id | doaj.art-738615a7c5884727a8a3555fc0fd15c6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T00:14:28Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-738615a7c5884727a8a3555fc0fd15c62023-03-16T06:23:49ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.11221721122172The relationship between gut microbiota and neonatal pathologic jaundice: A pilot case-control studyJia Jia You0Jia Jia You1Jia Jia You2Jun Qiu3Gui Nan Li4Xiao Ming Peng5Ye Ma6Chang Ci Zhou7Chang Ci Zhou8Chang Ci Zhou9Si Wei Fang10Si Wei Fang11Si Wei Fang12Rui Wen Huang13Zheng Hui Xiao14Pediatrics Research Institute of Hunan Province, Hunan Children’s Hospital, Changsha, ChinaAcademy of Pediatrics, Hengyang Medical School, University of South China, Hengyang, ChinaDepartment of Emergency Center, Hunan Children’s Hospital, Changsha, ChinaPediatrics Research Institute of Hunan Province, Hunan Children’s Hospital, Changsha, ChinaDepartment of Neonatology, Hunan Children’s Hospital, Changsha, ChinaDepartment of Neonatology, Hunan Children’s Hospital, Changsha, ChinaDepartment of Neonatology, Hunan Children’s Hospital, Changsha, ChinaPediatrics Research Institute of Hunan Province, Hunan Children’s Hospital, Changsha, ChinaAcademy of Pediatrics, Hengyang Medical School, University of South China, Hengyang, ChinaDepartment of Emergency Center, Hunan Children’s Hospital, Changsha, ChinaPediatrics Research Institute of Hunan Province, Hunan Children’s Hospital, Changsha, ChinaAcademy of Pediatrics, Hengyang Medical School, University of South China, Hengyang, ChinaDepartment of Emergency Center, Hunan Children’s Hospital, Changsha, ChinaDepartment of Neonatology, Hunan Children’s Hospital, Changsha, ChinaDepartment of Emergency Center, Hunan Children’s Hospital, Changsha, ChinaBackground and objectiveNeonatal jaundice is a common clinical disease in neonates. Pathologic jaundice is more harmful to neonates. There are a few studies on the biomarkers of pathologic jaundice and the correlation between gut microbiota and clinical indices. Therefore, we aimed to reveal the characteristics of gut microbiota in pathologic jaundice, provide potential biomarkers for the diagnosis of pathologic jaundice, and find the correlation between gut microbiota and clinical indices.MethodsFourteen neonates with physiologic jaundice were recruited into a control group (Group A). Additionally, 14 neonates with pathologic jaundice were recruited into a case group (Group B). The microbial communities were analyzed using 16S rDNA sequencing. LEfSe and the differences in the relative abundance of gut microbiota were used to identify different bacteria among the two groups. The ROC curve was used to assess effective biomarkers for pathologic jaundice. Spearman’s rank-sum correlation coefficient was used to evaluate the correlation between gut microbiota and clinical indices.ResultsThere were no differences in the total richness or diversity of gut microbiota between the two groups. At the phylum and genus levels, compared with the control group, Bacteroidetes (p = 0.002) and Braydrhizobium (p = 0.01) were significantly higher, while Actinobacteria (p = 0.003) and Bidfldobacterium (p = 0.016) were significantly lower in the case group. Bacteroidetes were valuable in differentiating pathologic jaundice from physiologic jaundice by the ROC curve, and the area under the ROC curve (AUC) value was 0.839 [95%CI (0.648–0.995)]. In the case group, Bacteroidetes were negatively associated with total bilirubin (TBIL) (p < 0.05). In the control group, Bacteroidetes were positively associated with TBIL (p < 0.05).ConclusionBacteroidetes could be used as biomarkers to identify pathologic jaundice and Bacteroidetes are positively associated with bilirubin levels.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1122172/fullBacteroidetesgut microbiotapathologic jaundicephysiologic jaundiceneonate |
| spellingShingle | Jia Jia You Jia Jia You Jia Jia You Jun Qiu Gui Nan Li Xiao Ming Peng Ye Ma Chang Ci Zhou Chang Ci Zhou Chang Ci Zhou Si Wei Fang Si Wei Fang Si Wei Fang Rui Wen Huang Zheng Hui Xiao The relationship between gut microbiota and neonatal pathologic jaundice: A pilot case-control study Frontiers in Microbiology Bacteroidetes gut microbiota pathologic jaundice physiologic jaundice neonate |
| title | The relationship between gut microbiota and neonatal pathologic jaundice: A pilot case-control study |
| title_full | The relationship between gut microbiota and neonatal pathologic jaundice: A pilot case-control study |
| title_fullStr | The relationship between gut microbiota and neonatal pathologic jaundice: A pilot case-control study |
| title_full_unstemmed | The relationship between gut microbiota and neonatal pathologic jaundice: A pilot case-control study |
| title_short | The relationship between gut microbiota and neonatal pathologic jaundice: A pilot case-control study |
| title_sort | relationship between gut microbiota and neonatal pathologic jaundice a pilot case control study |
| topic | Bacteroidetes gut microbiota pathologic jaundice physiologic jaundice neonate |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1122172/full |
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