Identification of hsa_circRNA_100632 as a novel molecular biomarker for fulminant type 1 diabetes
ObjectiveCircular RNAs (circRNAs) are associated with diabetes, but their role in fulminant type 1 diabetes (FT1D) is unclear. Thus, we characterized the role of circRNAs in FT1D.Research design and methodsCircRNA expression profiles were detected in peripheral blood mononuclear cells (PBMCs) of fiv...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1075970/full |
| _version_ | 1797896052712407040 |
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| author | Wenfeng Yin Shuoming Luo Junlin Qiu Zilin Xiao Ziwei Zhang Zhiguo Xie Xia Li Zhiguang Zhou |
| author_facet | Wenfeng Yin Shuoming Luo Junlin Qiu Zilin Xiao Ziwei Zhang Zhiguo Xie Xia Li Zhiguang Zhou |
| author_sort | Wenfeng Yin |
| collection | DOAJ |
| description | ObjectiveCircular RNAs (circRNAs) are associated with diabetes, but their role in fulminant type 1 diabetes (FT1D) is unclear. Thus, we characterized the role of circRNAs in FT1D.Research design and methodsCircRNA expression profiles were detected in peripheral blood mononuclear cells (PBMCs) of five FT1D patients and five controls using a circRNA microarray. An independent cohort comprised of 40 FT1D cases, 75 type 1 diabetes (T1D) cases, and 115 controls was used to verify the circRNAs using quantitative real-time polymerase chain reaction (qRT-PCR). Spearman’s correlation analysis and receiver operating characteristic (ROC) curve analysis were performed to determine the clinical diagnostic capability of circRNAs. Bioinformatics was used to identify potential biological functions and circRNA–miRNA–mRNA interactions.ResultsThere were 13 upregulated and 13 downregulated circRNAs in PBMCs of patients with FT1D. Five circRNAs were further verified in a second cohort. Hsa_circRNA_100632 was significantly upregulated in the FT1D and T1D groups. Hsa_circRNA_100632 was differentiated between patients with FT1D and controls [area under the curve (AUC) 0.846; 95% CI 0.776–0.916; P<0.0001] as well as between patients with FT1D and patients with T1D (AUC 0.726; 95% CI 0.633–0.820; P<0.0001). Bioinformatics analysis showed that hsa_circRNA_100632 may be involved in 47 circRNA–miRNA–mRNA signaling pathways associated with diabetes.ConclusionsCircRNAs were aberrantly expressed in PBMCs of patients with FT1D, and hsa_circRNA_100632 may be a diagnostic marker of FT1D. |
| first_indexed | 2024-04-10T07:36:43Z |
| format | Article |
| id | doaj.art-7386e804eb9e42e0b63d3b283817e384 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-10T07:36:43Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-7386e804eb9e42e0b63d3b283817e3842023-02-23T12:39:53ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-02-011410.3389/fimmu.2023.10759701075970Identification of hsa_circRNA_100632 as a novel molecular biomarker for fulminant type 1 diabetesWenfeng YinShuoming LuoJunlin QiuZilin XiaoZiwei ZhangZhiguo XieXia LiZhiguang ZhouObjectiveCircular RNAs (circRNAs) are associated with diabetes, but their role in fulminant type 1 diabetes (FT1D) is unclear. Thus, we characterized the role of circRNAs in FT1D.Research design and methodsCircRNA expression profiles were detected in peripheral blood mononuclear cells (PBMCs) of five FT1D patients and five controls using a circRNA microarray. An independent cohort comprised of 40 FT1D cases, 75 type 1 diabetes (T1D) cases, and 115 controls was used to verify the circRNAs using quantitative real-time polymerase chain reaction (qRT-PCR). Spearman’s correlation analysis and receiver operating characteristic (ROC) curve analysis were performed to determine the clinical diagnostic capability of circRNAs. Bioinformatics was used to identify potential biological functions and circRNA–miRNA–mRNA interactions.ResultsThere were 13 upregulated and 13 downregulated circRNAs in PBMCs of patients with FT1D. Five circRNAs were further verified in a second cohort. Hsa_circRNA_100632 was significantly upregulated in the FT1D and T1D groups. Hsa_circRNA_100632 was differentiated between patients with FT1D and controls [area under the curve (AUC) 0.846; 95% CI 0.776–0.916; P<0.0001] as well as between patients with FT1D and patients with T1D (AUC 0.726; 95% CI 0.633–0.820; P<0.0001). Bioinformatics analysis showed that hsa_circRNA_100632 may be involved in 47 circRNA–miRNA–mRNA signaling pathways associated with diabetes.ConclusionsCircRNAs were aberrantly expressed in PBMCs of patients with FT1D, and hsa_circRNA_100632 may be a diagnostic marker of FT1D.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1075970/fullcircular RNAsfulminant type 1 diabetesbiomarkersdiagnostic evaluationimmune regulation |
| spellingShingle | Wenfeng Yin Shuoming Luo Junlin Qiu Zilin Xiao Ziwei Zhang Zhiguo Xie Xia Li Zhiguang Zhou Identification of hsa_circRNA_100632 as a novel molecular biomarker for fulminant type 1 diabetes Frontiers in Immunology circular RNAs fulminant type 1 diabetes biomarkers diagnostic evaluation immune regulation |
| title | Identification of hsa_circRNA_100632 as a novel molecular biomarker for fulminant type 1 diabetes |
| title_full | Identification of hsa_circRNA_100632 as a novel molecular biomarker for fulminant type 1 diabetes |
| title_fullStr | Identification of hsa_circRNA_100632 as a novel molecular biomarker for fulminant type 1 diabetes |
| title_full_unstemmed | Identification of hsa_circRNA_100632 as a novel molecular biomarker for fulminant type 1 diabetes |
| title_short | Identification of hsa_circRNA_100632 as a novel molecular biomarker for fulminant type 1 diabetes |
| title_sort | identification of hsa circrna 100632 as a novel molecular biomarker for fulminant type 1 diabetes |
| topic | circular RNAs fulminant type 1 diabetes biomarkers diagnostic evaluation immune regulation |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1075970/full |
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