Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (<i>Lilrb4</i>) Expression in Microglia
As resident innate immune cells of the CNS, microglia play important essential roles during physiological and pathological situations. Recent reports have described the expression of <i>Lilrb4</i> in disease-associated and aged microglia. Here, we characterized the expression of <i>...
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MDPI AG
2021-12-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/10/12/1300 |
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| author | Felix Kretzschmar Robin Piecha Jannik Jahn Phani Sankar Potru Björn Spittau |
| author_facet | Felix Kretzschmar Robin Piecha Jannik Jahn Phani Sankar Potru Björn Spittau |
| author_sort | Felix Kretzschmar |
| collection | DOAJ |
| description | As resident innate immune cells of the CNS, microglia play important essential roles during physiological and pathological situations. Recent reports have described the expression of <i>Lilrb4</i> in disease-associated and aged microglia. Here, we characterized the expression of <i>Lilrb4</i> in microglia in vitro and in vivo in comparison with bone marrow-derived monocytes and peritoneal macrophages in mice. Using BV2 cells, primary microglia cultures as well as ex vivo isolated microglia and myeloid cells in combination with qPCR and flow cytometry, we were able to provide a comprehensive characterization of <i>Lilrb4</i> expression in distinct mouse myeloid cells. Whereas microglia in vivo display low expression of <i>Lilrb4</i>, primary microglia cultures present high levels of surface LILRB4. Among the analyzed peripheral myeloid cells, peritoneal macrophages showed the highest expression levels of <i>Lilrb4</i>. Moreover, LPS treatment and inhibition of microglial TGFβ signaling resulted in significant increases of LILRB4 cell surface levels. Taken together, our data indicate that LILRB4 is a reliable surface marker for activated microglia and further demonstrate that microglial TGFβ signaling is involved in the regulation of <i>Lilrb4</i> expression during LPS-induced microglia activation. |
| first_indexed | 2024-03-10T04:34:46Z |
| format | Article |
| id | doaj.art-7388edccecec4c8a9fa5eb566b815b46 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T04:34:46Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-7388edccecec4c8a9fa5eb566b815b462023-11-23T03:53:57ZengMDPI AGBiology2079-77372021-12-011012130010.3390/biology10121300Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (<i>Lilrb4</i>) Expression in MicrogliaFelix Kretzschmar0Robin Piecha1Jannik Jahn2Phani Sankar Potru3Björn Spittau4Institute of Anatomy, Medicine Rostock, University of Rostock, 18055 Rostock, GermanyInstitute of Anatomy, Medicine Rostock, University of Rostock, 18055 Rostock, GermanyInstitute of Anatomy, Medicine Rostock, University of Rostock, 18055 Rostock, GermanyInstitute of Anatomy, Medicine Rostock, University of Rostock, 18055 Rostock, GermanyInstitute of Anatomy, Medicine Rostock, University of Rostock, 18055 Rostock, GermanyAs resident innate immune cells of the CNS, microglia play important essential roles during physiological and pathological situations. Recent reports have described the expression of <i>Lilrb4</i> in disease-associated and aged microglia. Here, we characterized the expression of <i>Lilrb4</i> in microglia in vitro and in vivo in comparison with bone marrow-derived monocytes and peritoneal macrophages in mice. Using BV2 cells, primary microglia cultures as well as ex vivo isolated microglia and myeloid cells in combination with qPCR and flow cytometry, we were able to provide a comprehensive characterization of <i>Lilrb4</i> expression in distinct mouse myeloid cells. Whereas microglia in vivo display low expression of <i>Lilrb4</i>, primary microglia cultures present high levels of surface LILRB4. Among the analyzed peripheral myeloid cells, peritoneal macrophages showed the highest expression levels of <i>Lilrb4</i>. Moreover, LPS treatment and inhibition of microglial TGFβ signaling resulted in significant increases of LILRB4 cell surface levels. Taken together, our data indicate that LILRB4 is a reliable surface marker for activated microglia and further demonstrate that microglial TGFβ signaling is involved in the regulation of <i>Lilrb4</i> expression during LPS-induced microglia activation.https://www.mdpi.com/2079-7737/10/12/1300Lilrb4microgliaTGFβ1LPS |
| spellingShingle | Felix Kretzschmar Robin Piecha Jannik Jahn Phani Sankar Potru Björn Spittau Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (<i>Lilrb4</i>) Expression in Microglia Biology Lilrb4 microglia TGFβ1 LPS |
| title | Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (<i>Lilrb4</i>) Expression in Microglia |
| title_full | Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (<i>Lilrb4</i>) Expression in Microglia |
| title_fullStr | Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (<i>Lilrb4</i>) Expression in Microglia |
| title_full_unstemmed | Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (<i>Lilrb4</i>) Expression in Microglia |
| title_short | Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (<i>Lilrb4</i>) Expression in Microglia |
| title_sort | characterization of the leucocyte immunoglobulin like receptor b4 i lilrb4 i expression in microglia |
| topic | Lilrb4 microglia TGFβ1 LPS |
| url | https://www.mdpi.com/2079-7737/10/12/1300 |
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