CRISPR/Cas9-based generation of a recombinant double-reporter pseudorabies virus and its characterization in vitro and in vivo

CRISPR/Cas9-based generation of a recombinant double-reporter pseudorabies virus and its characterization in vitro and in vivo

Abstract Pseudorabies, caused by pseudorabies virus (PRV) variants, has broken out among commercial PRV vaccine-immunized swine herds and resulted in major economic losses to the pig industry in China since late 2011. However, the mechanism of virulence enhancement of variant PRV is currently unclea...

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Bibliographic Details
Main Authors: Peng-Fei Fu, Xuan Cheng, Bing-Qian Su, Li-Fang Duan, Cong-Rong Wang, Xin-Rui Niu, Jiang Wang, Guo-Yu Yang, Bei-Bei Chu
Format: Article
Language:English
Published: BMC 2021-06-01
Series:Veterinary Research
Subjects:
Pseudorabies virus
Firefly luciferase
EGFP
CRISPR/Cas9
Imaging in vivo
25-hydroxycholesterol
Online Access:https://doi.org/10.1186/s13567-021-00964-4

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