| Summary: | Ottelia cordata (Wallich) Dandy (Hydrocharitaceae) is an endangered aquatic macrophyte. The complete chloroplast genome sequence of O. cordata was generated by de novo assembly using whole genome next-generation sequencing. The complete chloroplast genome was 157,879 bp in length and constructed out of four parts – a large single-copy (LSC) region of 87,672 bp, a small single-copy (SSC) region of 20,265 bp, and two inverted repeat (IRa and IRb) regions of 24,971 bp each. The genome annotation predicted a total of 117 genes, including 72 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis of 20 selected chloroplast genomes revealed that sampled species of Hydrocharitaceae formed a monophyletic clade, in which O. cordata and O. acuminata var. songmingensis formed a sister clade to Elodea canadensis.
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