BIOLOGICAL ACTIVITY OF ANTIMICROBIAL PEPTIDES OF ENTEROCOCCUS FAECIUM
Aim. Isolate bacteriocins from Enterococcus faecium metabolites and characterize their effect on cells of Gram positive (Listeria monocytogenes) and Gram negative (Escherichia coli) bacteria. Materials and methods. Methods of solid-phase extraction, ion-exchange and reverse-phase chromatography were...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2015-08-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.crie.ru/jour/article/view/14056 |
| _version_ | 1797782210458157056 |
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| author | A. S Vasilchenko E. A Rogozhin A. V Valyshev |
| author_facet | A. S Vasilchenko E. A Rogozhin A. V Valyshev |
| author_sort | A. S Vasilchenko |
| collection | DOAJ |
| description | Aim. Isolate bacteriocins from Enterococcus faecium metabolites and characterize their effect on cells of Gram positive (Listeria monocytogenes) and Gram negative (Escherichia coli) bacteria. Materials and methods. Methods of solid-phase extraction, ion-exchange and reverse-phase chromatography were applied for isolation of bacteriocins from cultural medium of bacteria. MALDI time-of-flight mass-spectrometry was used for characterization of the obtained preparations. The mechanism ofbiological effect of peptides was evaluated using DNA-tropic dyes (SYTO 9 and PI) with subsequent registration of fluorescence spectra. Atomic-force microscopy (AFM) was used for characterization of morpho-functional reaction of target cells. Results. Peptide fractions with mass of 1.0 - 3.0 kDa were isolated from enterococci metabolites, that inhibit the growth of indicator microorganisms. E. faecium strain exoproducts were shown to increase membrane permeability during interaction with L. monocytogenes, that results in subsequent detectable disturbance of normal cell morphology of listeria. Alterations of E. coli surface during the effect of purified peptide fraction was detected using AFM. Conclusion. The studies carried out have revealed the effect ofbacteriocins of enterococci on microorganisms with various types of cell wall composition and have confirmed the importance of bacterial barrier structure permeability disturbance in the mechanism of antimicrobial effect of enterocins. |
| first_indexed | 2024-03-13T00:07:47Z |
| format | Article |
| id | doaj.art-738d0c6152d140668b9bf9a1d70ffd56 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-03-13T00:07:47Z |
| publishDate | 2015-08-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-738d0c6152d140668b9bf9a1d70ffd562023-07-12T20:19:38ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132015-08-0192422262481BIOLOGICAL ACTIVITY OF ANTIMICROBIAL PEPTIDES OF ENTEROCOCCUS FAECIUMA. S Vasilchenko0E. A Rogozhin1A. V Valyshev2Research Institute of Cellular and Intracellular SymbiosisSchemyakin and Ovchinnikov Research Institute of Bioorganic ChemistryResearch Institute of Cellular and Intracellular SymbiosisAim. Isolate bacteriocins from Enterococcus faecium metabolites and characterize their effect on cells of Gram positive (Listeria monocytogenes) and Gram negative (Escherichia coli) bacteria. Materials and methods. Methods of solid-phase extraction, ion-exchange and reverse-phase chromatography were applied for isolation of bacteriocins from cultural medium of bacteria. MALDI time-of-flight mass-spectrometry was used for characterization of the obtained preparations. The mechanism ofbiological effect of peptides was evaluated using DNA-tropic dyes (SYTO 9 and PI) with subsequent registration of fluorescence spectra. Atomic-force microscopy (AFM) was used for characterization of morpho-functional reaction of target cells. Results. Peptide fractions with mass of 1.0 - 3.0 kDa were isolated from enterococci metabolites, that inhibit the growth of indicator microorganisms. E. faecium strain exoproducts were shown to increase membrane permeability during interaction with L. monocytogenes, that results in subsequent detectable disturbance of normal cell morphology of listeria. Alterations of E. coli surface during the effect of purified peptide fraction was detected using AFM. Conclusion. The studies carried out have revealed the effect ofbacteriocins of enterococci on microorganisms with various types of cell wall composition and have confirmed the importance of bacterial barrier structure permeability disturbance in the mechanism of antimicrobial effect of enterocins.https://microbiol.crie.ru/jour/article/view/14056enterococciantimicrobial peptidesbacteriocinsenterocins |
| spellingShingle | A. S Vasilchenko E. A Rogozhin A. V Valyshev BIOLOGICAL ACTIVITY OF ANTIMICROBIAL PEPTIDES OF ENTEROCOCCUS FAECIUM Журнал микробиологии, эпидемиологии и иммунобиологии enterococci antimicrobial peptides bacteriocins enterocins |
| title | BIOLOGICAL ACTIVITY OF ANTIMICROBIAL PEPTIDES OF ENTEROCOCCUS FAECIUM |
| title_full | BIOLOGICAL ACTIVITY OF ANTIMICROBIAL PEPTIDES OF ENTEROCOCCUS FAECIUM |
| title_fullStr | BIOLOGICAL ACTIVITY OF ANTIMICROBIAL PEPTIDES OF ENTEROCOCCUS FAECIUM |
| title_full_unstemmed | BIOLOGICAL ACTIVITY OF ANTIMICROBIAL PEPTIDES OF ENTEROCOCCUS FAECIUM |
| title_short | BIOLOGICAL ACTIVITY OF ANTIMICROBIAL PEPTIDES OF ENTEROCOCCUS FAECIUM |
| title_sort | biological activity of antimicrobial peptides of enterococcus faecium |
| topic | enterococci antimicrobial peptides bacteriocins enterocins |
| url | https://microbiol.crie.ru/jour/article/view/14056 |
| work_keys_str_mv | AT asvasilchenko biologicalactivityofantimicrobialpeptidesofenterococcusfaecium AT earogozhin biologicalactivityofantimicrobialpeptidesofenterococcusfaecium AT avvalyshev biologicalactivityofantimicrobialpeptidesofenterococcusfaecium |