Marker-Assisted Pyramiding of Blast-Resistance Genes in a <i>japonica</i> Elite Rice Cultivar through Forward and Background Selection
Rice blast, caused by <i>Pyricularia oryzae</i>, is one of the main rice diseases worldwide. The pyramiding of blast-resistance (<i>Pi</i>) genes, coupled to Marker-Assisted BackCrossing (MABC), provides broad-spectrum and potentially durable resistance while limiting the don...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/4/757 |
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| author | Elisa Zampieri Andrea Volante Caterina Marè Gabriele Orasen Francesca Desiderio Chiara Biselli Marco Canella Lorena Carmagnola Joëlle Milazzo Henri Adreit Didier Tharreau Nicolas Poncelet Patrizia Vaccino Giampiero Valè |
| author_facet | Elisa Zampieri Andrea Volante Caterina Marè Gabriele Orasen Francesca Desiderio Chiara Biselli Marco Canella Lorena Carmagnola Joëlle Milazzo Henri Adreit Didier Tharreau Nicolas Poncelet Patrizia Vaccino Giampiero Valè |
| author_sort | Elisa Zampieri |
| collection | DOAJ |
| description | Rice blast, caused by <i>Pyricularia oryzae</i>, is one of the main rice diseases worldwide. The pyramiding of blast-resistance (<i>Pi</i>) genes, coupled to Marker-Assisted BackCrossing (MABC), provides broad-spectrum and potentially durable resistance while limiting the donor genome in the background of an elite cultivar. In this work, MABC coupled to foreground and background selections based on KASP marker assays has been applied to introgress four <i>Pi</i> genes (<i>Piz, Pib, Pita</i>, and <i>Pik</i>) in a renowned <i>japonica</i> Italian rice variety, highly susceptible to blast. Molecular analyses on the backcross (BC) lines highlighted the presence of an additional blast-resistance gene, the <i>Pita</i>-linked <i>Pita2</i>/<i>Ptr</i> gene, therefore increasing the number of blast-resistance introgressed genes to five. The recurrent genome was recovered up to 95.65%. Several lines carrying four (including <i>Pita2</i>) <i>Pi</i> genes with high recovery percentage levels were also obtained. Phenotypic evaluations confirmed the effectiveness of the pyramided lines against multivirulent strains, which also had broad patterns of resistance in comparison to those expected based on the pyramided <i>Pi</i> genes. The developed blast-resistant <i>japonica</i> lines represent useful donors of multiple blast-resistance genes for future rice-breeding programs related to the <i>japonica</i> group. |
| first_indexed | 2024-03-11T08:14:53Z |
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| id | doaj.art-738d968d23f14907aec46d04d8dae40f |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T08:14:53Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-738d968d23f14907aec46d04d8dae40f2023-11-16T22:47:04ZengMDPI AGPlants2223-77472023-02-0112475710.3390/plants12040757Marker-Assisted Pyramiding of Blast-Resistance Genes in a <i>japonica</i> Elite Rice Cultivar through Forward and Background SelectionElisa Zampieri0Andrea Volante1Caterina Marè2Gabriele Orasen3Francesca Desiderio4Chiara Biselli5Marco Canella6Lorena Carmagnola7Joëlle Milazzo8Henri Adreit9Didier Tharreau10Nicolas Poncelet11Patrizia Vaccino12Giampiero Valè13Council for Agricultural Research and Economics—Research Centre for Cereal and Industrial Crops, s.s. 11 to Torino, km 2.5, 13100 Vercelli, VC, ItalyCouncil for Agricultural Research and Economics—Research Centre for Cereal and Industrial Crops, s.s. 11 to Torino, km 2.5, 13100 Vercelli, VC, ItalyCouncil for Agricultural Research and Economics—Research Centre for Genomics and Bioinformatics, Via S. Protaso 302, 29017 Fiorenzuola d’Arda, PC, ItalyBertone Sementi S.P.A., Strada Cacciolo, 15030 Terruggia, AL, ItalyCouncil for Agricultural Research and Economics—Research Centre for Genomics and Bioinformatics, Via S. Protaso 302, 29017 Fiorenzuola d’Arda, PC, ItalyCouncil for Agricultural Research and Economics—Viticulture and Oenology, Viale Santa Margherita 80, 52100 Arezzo, AR, ItalyCouncil for Agricultural Research and Economics—Research Centre for Cereal and Industrial Crops, s.s. 11 to Torino, km 2.5, 13100 Vercelli, VC, ItalyCouncil for Agricultural Research and Economics—Research Centre for Cereal and Industrial Crops, s.s. 11 to Torino, km 2.5, 13100 Vercelli, VC, ItalyCIRAD, UMR PHIM TA A 120/K, Campus de Baillarguet, 34, CEDEX 5, 34398 Montpellier, FranceCIRAD, UMR PHIM TA A 120/K, Campus de Baillarguet, 34, CEDEX 5, 34398 Montpellier, FranceCIRAD, UMR PHIM TA A 120/K, Campus de Baillarguet, 34, CEDEX 5, 34398 Montpellier, FranceCIRAD, UMR PHIM TA A 120/K, Campus de Baillarguet, 34, CEDEX 5, 34398 Montpellier, FranceCouncil for Agricultural Research and Economics—Research Centre for Cereal and Industrial Crops, s.s. 11 to Torino, km 2.5, 13100 Vercelli, VC, ItalyCouncil for Agricultural Research and Economics—Research Centre for Cereal and Industrial Crops, s.s. 11 to Torino, km 2.5, 13100 Vercelli, VC, ItalyRice blast, caused by <i>Pyricularia oryzae</i>, is one of the main rice diseases worldwide. The pyramiding of blast-resistance (<i>Pi</i>) genes, coupled to Marker-Assisted BackCrossing (MABC), provides broad-spectrum and potentially durable resistance while limiting the donor genome in the background of an elite cultivar. In this work, MABC coupled to foreground and background selections based on KASP marker assays has been applied to introgress four <i>Pi</i> genes (<i>Piz, Pib, Pita</i>, and <i>Pik</i>) in a renowned <i>japonica</i> Italian rice variety, highly susceptible to blast. Molecular analyses on the backcross (BC) lines highlighted the presence of an additional blast-resistance gene, the <i>Pita</i>-linked <i>Pita2</i>/<i>Ptr</i> gene, therefore increasing the number of blast-resistance introgressed genes to five. The recurrent genome was recovered up to 95.65%. Several lines carrying four (including <i>Pita2</i>) <i>Pi</i> genes with high recovery percentage levels were also obtained. Phenotypic evaluations confirmed the effectiveness of the pyramided lines against multivirulent strains, which also had broad patterns of resistance in comparison to those expected based on the pyramided <i>Pi</i> genes. The developed blast-resistant <i>japonica</i> lines represent useful donors of multiple blast-resistance genes for future rice-breeding programs related to the <i>japonica</i> group.https://www.mdpi.com/2223-7747/12/4/757<i>Pyricularia oryzae</i><i>Pi</i> genespyramidingbackcrossricemolecular markers |
| spellingShingle | Elisa Zampieri Andrea Volante Caterina Marè Gabriele Orasen Francesca Desiderio Chiara Biselli Marco Canella Lorena Carmagnola Joëlle Milazzo Henri Adreit Didier Tharreau Nicolas Poncelet Patrizia Vaccino Giampiero Valè Marker-Assisted Pyramiding of Blast-Resistance Genes in a <i>japonica</i> Elite Rice Cultivar through Forward and Background Selection Plants <i>Pyricularia oryzae</i> <i>Pi</i> genes pyramiding backcross rice molecular markers |
| title | Marker-Assisted Pyramiding of Blast-Resistance Genes in a <i>japonica</i> Elite Rice Cultivar through Forward and Background Selection |
| title_full | Marker-Assisted Pyramiding of Blast-Resistance Genes in a <i>japonica</i> Elite Rice Cultivar through Forward and Background Selection |
| title_fullStr | Marker-Assisted Pyramiding of Blast-Resistance Genes in a <i>japonica</i> Elite Rice Cultivar through Forward and Background Selection |
| title_full_unstemmed | Marker-Assisted Pyramiding of Blast-Resistance Genes in a <i>japonica</i> Elite Rice Cultivar through Forward and Background Selection |
| title_short | Marker-Assisted Pyramiding of Blast-Resistance Genes in a <i>japonica</i> Elite Rice Cultivar through Forward and Background Selection |
| title_sort | marker assisted pyramiding of blast resistance genes in a i japonica i elite rice cultivar through forward and background selection |
| topic | <i>Pyricularia oryzae</i> <i>Pi</i> genes pyramiding backcross rice molecular markers |
| url | https://www.mdpi.com/2223-7747/12/4/757 |
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