MlrA, a MerR family regulator in Vibrio cholerae, senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonization
Vibrio cholerae (V. cholerae), one of the most important bacterial pathogens in history, is a gram-negative motile bacterium that causes fatal pandemic disease in humans via oral ingestion of contaminated water or food. This process involves the coordinated actions of numerous regulatory factors. Th...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2022.2143216 |
| _version_ | 1811220398306492416 |
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| author | Jialin Wu Yutao Liu Wendi Li Fan Li Ruiying Liu Hao Sun Jingliang Qin Xiaohui Feng Di Huang Bin Liu |
| author_facet | Jialin Wu Yutao Liu Wendi Li Fan Li Ruiying Liu Hao Sun Jingliang Qin Xiaohui Feng Di Huang Bin Liu |
| author_sort | Jialin Wu |
| collection | DOAJ |
| description | Vibrio cholerae (V. cholerae), one of the most important bacterial pathogens in history, is a gram-negative motile bacterium that causes fatal pandemic disease in humans via oral ingestion of contaminated water or food. This process involves the coordinated actions of numerous regulatory factors. The MerR family regulators, which are widespread in prokaryotes, have been reported to be associated with pathogenicity. However, the role of the MerR family regulators in V. cholerae virulence remains unknown. Our study systematically investigated the influence of MerR family regulators on intestinal colonization of V. cholerae within the host. Among the five MerR family regulators, MlrA was found to significantly promote the colonization capacity of V. cholerae in infant mice. Furthermore, we revealed that MlrA increases bacterial intestinal colonization by directly enhancing the expression of tcpA, which encodes one of the most important virulence factors in V. cholerae, by binding to its promoter region. In addition, we revealed that during infection, mlrA is activated by anaerobic signals in the small intestine of the host through Fnr. In summary, our findings reveal a MlrA-mediated virulence regulation pathway that enables V. cholerae to sense environmental signals at the infection site to precisely activate virulence gene expression, thus providing useful insights into the pathogenic mechanisms of V. cholerae. |
| first_indexed | 2024-04-12T07:42:15Z |
| format | Article |
| id | doaj.art-738fa04742e3486190ed612e9a3569af |
| institution | Directory Open Access Journal |
| issn | 1949-0976 1949-0984 |
| language | English |
| last_indexed | 2024-04-12T07:42:15Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj.art-738fa04742e3486190ed612e9a3569af2022-12-22T03:41:48ZengTaylor & Francis GroupGut Microbes1949-09761949-09842022-12-0114110.1080/19490976.2022.2143216MlrA, a MerR family regulator in Vibrio cholerae, senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonizationJialin Wu0Yutao Liu1Wendi Li2Fan Li3Ruiying Liu4Hao Sun5Jingliang Qin6Xiaohui Feng7Di Huang8Bin Liu9TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaTEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaTEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaTEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaTEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaTEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaTEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaTEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaTEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaTEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaVibrio cholerae (V. cholerae), one of the most important bacterial pathogens in history, is a gram-negative motile bacterium that causes fatal pandemic disease in humans via oral ingestion of contaminated water or food. This process involves the coordinated actions of numerous regulatory factors. The MerR family regulators, which are widespread in prokaryotes, have been reported to be associated with pathogenicity. However, the role of the MerR family regulators in V. cholerae virulence remains unknown. Our study systematically investigated the influence of MerR family regulators on intestinal colonization of V. cholerae within the host. Among the five MerR family regulators, MlrA was found to significantly promote the colonization capacity of V. cholerae in infant mice. Furthermore, we revealed that MlrA increases bacterial intestinal colonization by directly enhancing the expression of tcpA, which encodes one of the most important virulence factors in V. cholerae, by binding to its promoter region. In addition, we revealed that during infection, mlrA is activated by anaerobic signals in the small intestine of the host through Fnr. In summary, our findings reveal a MlrA-mediated virulence regulation pathway that enables V. cholerae to sense environmental signals at the infection site to precisely activate virulence gene expression, thus providing useful insights into the pathogenic mechanisms of V. cholerae.https://www.tandfonline.com/doi/10.1080/19490976.2022.2143216Vibrio choleraeMerR family regulatortoxin-coregulated pilusMlrApathogenicity |
| spellingShingle | Jialin Wu Yutao Liu Wendi Li Fan Li Ruiying Liu Hao Sun Jingliang Qin Xiaohui Feng Di Huang Bin Liu MlrA, a MerR family regulator in Vibrio cholerae, senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonization Gut Microbes Vibrio cholerae MerR family regulator toxin-coregulated pilus MlrA pathogenicity |
| title | MlrA, a MerR family regulator in Vibrio cholerae, senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonization |
| title_full | MlrA, a MerR family regulator in Vibrio cholerae, senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonization |
| title_fullStr | MlrA, a MerR family regulator in Vibrio cholerae, senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonization |
| title_full_unstemmed | MlrA, a MerR family regulator in Vibrio cholerae, senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonization |
| title_short | MlrA, a MerR family regulator in Vibrio cholerae, senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonization |
| title_sort | mlra a merr family regulator in vibrio cholerae senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonization |
| topic | Vibrio cholerae MerR family regulator toxin-coregulated pilus MlrA pathogenicity |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2022.2143216 |
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