Differential expression of miRNAs are associated with the insulin signaling pathway in preeclampsia and gestational hypertension
Objectives: The objective of this study was to determine microRNAs (miRNAs) expression levels in placental tissue and serum samples from preeclampsia (PE) and gestational hypertensive (GH) patients. Study design: Using a targeted qPCR method, the selected miRNAs putatively involved in the PE and GH...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-11-01
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| Series: | Clinical and Experimental Hypertension |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10641963.2018.1431257 |
| _version_ | 1797681378174697472 |
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| author | Olive P Khaliq Saravanakumar Murugesan Jagidesa Moodley Irene Mackraj |
| author_facet | Olive P Khaliq Saravanakumar Murugesan Jagidesa Moodley Irene Mackraj |
| author_sort | Olive P Khaliq |
| collection | DOAJ |
| description | Objectives: The objective of this study was to determine microRNAs (miRNAs) expression levels in placental tissue and serum samples from preeclampsia (PE) and gestational hypertensive (GH) patients. Study design: Using a targeted qPCR method, the selected miRNAs putatively involved in the PE and GH were examined from normotensive (n = 32), PE (n = 32) and GH (n = 28) in South African women. Western blot analysis of protein expressions of AKT and PI3K was performed in the placental tissue of all three groups. Results: qPCR results of serum miR-222 expression levels showed a significant decrease in PE compared to GH and normotensive groups. miR-29a expression levels were significantly increased in PE and GH groups compared to normotensives. Serum expression levels of miR-181a in GH showed a significant increase compared to the PE and normotensive groups. Placental tissue expression levels of miR-181a were significantly increased in PE and GH groups compared to normotensives. Western blot results of placental tissue showed a decrease in the expression levels of AKT-serine and threonine in the PE groups compared to the normotensives and a significantly higher expression in the GH groups compared to normotensives. Phosphatidyl-inositol-3 kinase (PI3K) expression levels were significantly decreased in PE and GH groups compared to normotensives. Conclusion: The present study, interestingly, demonstrates the differential expression of circulating miRNA in GH and a correlation between the expression levels of miRNAs with AKT/PI3K in the insulin signaling pathway, reinforcing the presence of metabolic dysregulation in PE and GH. |
| first_indexed | 2024-03-11T23:44:05Z |
| format | Article |
| id | doaj.art-7393da5656cf428b9694eecd09c8700a |
| institution | Directory Open Access Journal |
| issn | 1064-1963 1525-6006 |
| language | English |
| last_indexed | 2024-03-11T23:44:05Z |
| publishDate | 2018-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Clinical and Experimental Hypertension |
| spelling | doaj.art-7393da5656cf428b9694eecd09c8700a2023-09-19T15:19:26ZengTaylor & Francis GroupClinical and Experimental Hypertension1064-19631525-60062018-11-0140874475110.1080/10641963.2018.14312571431257Differential expression of miRNAs are associated with the insulin signaling pathway in preeclampsia and gestational hypertensionOlive P Khaliq0Saravanakumar Murugesan1Jagidesa Moodley2Irene Mackraj3University of KwaZulu-NatalUniversity of KwaZulu-NatalUniversity of KwaZulu-NatalUniversity of KwaZulu-NatalObjectives: The objective of this study was to determine microRNAs (miRNAs) expression levels in placental tissue and serum samples from preeclampsia (PE) and gestational hypertensive (GH) patients. Study design: Using a targeted qPCR method, the selected miRNAs putatively involved in the PE and GH were examined from normotensive (n = 32), PE (n = 32) and GH (n = 28) in South African women. Western blot analysis of protein expressions of AKT and PI3K was performed in the placental tissue of all three groups. Results: qPCR results of serum miR-222 expression levels showed a significant decrease in PE compared to GH and normotensive groups. miR-29a expression levels were significantly increased in PE and GH groups compared to normotensives. Serum expression levels of miR-181a in GH showed a significant increase compared to the PE and normotensive groups. Placental tissue expression levels of miR-181a were significantly increased in PE and GH groups compared to normotensives. Western blot results of placental tissue showed a decrease in the expression levels of AKT-serine and threonine in the PE groups compared to the normotensives and a significantly higher expression in the GH groups compared to normotensives. Phosphatidyl-inositol-3 kinase (PI3K) expression levels were significantly decreased in PE and GH groups compared to normotensives. Conclusion: The present study, interestingly, demonstrates the differential expression of circulating miRNA in GH and a correlation between the expression levels of miRNAs with AKT/PI3K in the insulin signaling pathway, reinforcing the presence of metabolic dysregulation in PE and GH.http://dx.doi.org/10.1080/10641963.2018.1431257mirnaspreeclampsiagestational hypertensionakt-serinephosphatidyl-inositol-3 |
| spellingShingle | Olive P Khaliq Saravanakumar Murugesan Jagidesa Moodley Irene Mackraj Differential expression of miRNAs are associated with the insulin signaling pathway in preeclampsia and gestational hypertension Clinical and Experimental Hypertension mirnas preeclampsia gestational hypertension akt-serine phosphatidyl-inositol-3 |
| title | Differential expression of miRNAs are associated with the insulin signaling pathway in preeclampsia and gestational hypertension |
| title_full | Differential expression of miRNAs are associated with the insulin signaling pathway in preeclampsia and gestational hypertension |
| title_fullStr | Differential expression of miRNAs are associated with the insulin signaling pathway in preeclampsia and gestational hypertension |
| title_full_unstemmed | Differential expression of miRNAs are associated with the insulin signaling pathway in preeclampsia and gestational hypertension |
| title_short | Differential expression of miRNAs are associated with the insulin signaling pathway in preeclampsia and gestational hypertension |
| title_sort | differential expression of mirnas are associated with the insulin signaling pathway in preeclampsia and gestational hypertension |
| topic | mirnas preeclampsia gestational hypertension akt-serine phosphatidyl-inositol-3 |
| url | http://dx.doi.org/10.1080/10641963.2018.1431257 |
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