Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods

Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods

Extracellular vesicles (EVs) are a versatile group of cell-secreted membranous nanoparticles present in body fluids. They have an exceptional diagnostic potential due to their molecular content matching the originating cells and accessibility from body fluids. However, methods for EV isolation are s...

Full description

Bibliographic Details
Main Authors:	Vedrana Krušić Alić, Mladenka Malenica, Maša Biberić, Siniša Zrna, Lara Valenčić, Aleksandar Šuput, Lada Kalagac Fabris, Karmen Wechtersbach, Nika Kojc, Mario Kurtjak, Natalia Kučić, Kristina Grabušić
Format:	Article
Language:	English
Published:	MDPI AG 2022-03-01
Series:	Biomedicines
Subjects:	extracellular vesicles cerebrospinal fluid traumatic brain injury size exclusion chromatography CD9 protein CD81 protein
Online Access:	https://www.mdpi.com/2227-9059/10/4/785

Similar Items

Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury
by: Lara Valenčić Seršić, et al.
Published: (2023-03-01)

Unveiling the Native Morphology of Extracellular Vesicles from Human Cerebrospinal Fluid by Atomic Force and Cryogenic Electron Microscopy
by: Mario Kurtjak, et al.
Published: (2022-05-01)

New Concept of Fusion Technics in Regional Anesthesia
by: Lada Kalagac Fabris, et al.
Published: (2022-01-01)

In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus
by: Juliana Lara Padovani, et al.
Published: (2013-02-01)

In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus
by: Juliana Lara Padovani, et al.
Published: (2013-04-01)

CD81 and CD9 work independently as extracellular components upon fusion of sperm and oocyte
by: Naoko Ohnami, et al.
Published: (2012-05-01)

Tetraspanins: Host Factors in Viral Infections
by: ChihSheng New, et al.
Published: (2021-10-01)

CD39 and CD326 Are Bona Fide Markers of Murine and Human Plasma Cells and Identify a Bone Marrow Specific Plasma Cell Subpopulation in Lupus
by: Van Duc Dang, et al.
Published: (2022-04-01)

Changes in Soluble Serum CD81 Concentration during an Oral Glucose Tolerance Test in Patients with Diabetes Mellitus and Individuals with Normal Glucose Tolerance
by: Seon Mee Kang, et al.
Published: (2023-11-01)

Machine learning-assisted elucidation of CD81–CD44 interactions in promoting cancer stemness and extracellular vesicle integrity
by: Erika K Ramos, et al.
Published: (2022-10-01)

Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus
by: Dmitry Polyakov, et al.
Published: (2021-05-01)

Differential proteomics argues against a general role for CD9, CD81 or CD63 in the sorting of proteins into extracellular vesicles
by: Yé Fan, et al.
Published: (2023-08-01)

Screening of EWI-2-Derived Peptides for Targeting Tetraspanin CD81 and Their Effect on Cancer Cell Migration
by: Thanawat Suwatthanarak, et al.
Published: (2023-03-01)

Recombinant hepatitis C virus-envelope protein 2 interactions with low-density lipoprotein/CD81 receptors
by: Ana Carolina Urbaczek, et al.
Published: (2015-06-01)

A Role for CD81 and Hepatitis C Virus in Hepatoma Mobility
by: Claire L. Brimacombe, et al.
Published: (2014-03-01)

CD81 binding regions of hepatitis C virus remain conserved after liver transplantation
by: Andre C. Lyra, et al.

Characterization and Oxidation Behaviour of Al2O3 Coating Deposited on Ti-Al-Nb Alloy in Air and in 9%O2 + 0.2%HCl + 0.08%SO2 + N2 Atmosphere
by: Małecka Joanna
Published: (2015-02-01)

Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids
by: Mladenka Malenica, et al.
Published: (2021-05-01)

Expression and functions of cluster of differentiation 9 and 81 in rat mammary epithelial cells
by: Kotaro HORIGUCHI, et al.
Published: (2020-08-01)

CD82 and Gangliosides Tune CD81 Membrane Behavior
by: Laurent Fernandez, et al.
Published: (2021-08-01)

Combination of High-Resolution Structures for the B Cell Receptor and Co-Receptors Provides an Understanding of Their Interactions with Therapeutic Antibodies
by: Puja Bhattacharyya, et al.
Published: (2023-05-01)

SHP-2 Interacts with CD81 and Regulates the Malignant Evolution of Colorectal Cancer by Inhibiting Epithelial–Mesenchymal Transition [Retraction]
by: Yuan H, et al.
Published: (2024-02-01)

Inhibiting uptake of extracellular vesicles derived from senescent bone marrow mesenchymal stem cells by muscle satellite cells attenuates sarcopenia
by: Hanhao Dai, et al.
Published: (2022-07-01)

Thyroiditis, secondary to viral infection.
by: Dr Husnia I Marrif
Published: (2010-12-01)

SHP-2 Interacts with CD81 and Regulates the Malignant Evolution of Colorectal Cancer by Inhibiting Epithelial–Mesenchymal Transition
by: Yuan H, et al.
Published: (2020-12-01)

Kajian penjerapan protin & amiloghikosidase keatas bahan penjerap deae-sepharose /
by: 351105 Khairul Anuar Khalid
Published: (1989)

Clinical Significance of BTLA, CD27, CD70, CD28 and CD80 as Diagnostic and Prognostic Markers in Ovarian Cancer
by: Janina Świderska, et al.
Published: (2022-01-01)

N-Glycosylation of Lipocalin 2 Is Not Required for Secretion or Exosome Targeting
by: Erawan Borkham-Kamphorst, et al.
Published: (2018-04-01)

The Tetraspanin CD81 Is a Host Factor for Chikungunya Virus Replication
by: Lisa Lasswitz, et al.
Published: (2022-06-01)

Genetically Engineered Extracellular Vesicles Harboring Transmembrane Scaffolds Exhibit Differences in Their Size, Expression Levels of Specific Surface Markers and Cell-Uptake
by: Jiayi Zhang, et al.
Published: (2022-11-01)

Comparative marker analysis of extracellular vesicles in different human cancer types
by: Yusuke Yoshioka, et al.
Published: (2013-06-01)

Increased circulating platelet-derived extracellular vesicles in severe COVID-19 disease
by: Tuukka Helin, et al.
Published: (2024-12-01)

Kajian penjerapan protein & amiloglukosidase ke atas bahan penjerap deae - sepharose / [mikrofilem]
by: 351105 Khairul Anuar Khalid

Targeting protein–protein interaction for immunomodulation: A sunflower trypsin inhibitor analog peptidomimetic suppresses RA progression in CIA model
by: Achyut Dahal, et al.
Published: (2022-07-01)

LincRNA01703 Facilitates CD81<sup>+</sup> Exosome Secretion to Inhibit Lung Adenocarcinoma Metastasis via the Rab27a/SYTL1/CD81 Complex
by: Yun Huang, et al.
Published: (2023-12-01)

CD81 Enhances Radioresistance of Glioblastoma by Promoting Nuclear Translocation of Rad51
by: Wang Zheng, et al.
Published: (2021-04-01)

Hydroxychloroquine induces apoptosis of myeloid-derived suppressor cells via up-regulation of CD81 contributing to alleviate lupus symptoms
by: Jiali Ni, et al.
Published: (2022-06-01)

Pseudotyping retrovirus like particles vaccine candidates with Hepatitis C virus envelope protein E2 requires the cellular expression of CD81
by: Hugo R. Soares, et al.
Published: (2019-02-01)

Design of Inhibitory Peptides of the Interaction between the E2 Protein of the Hepatitis C Virus and the CD81 and CD209 receptors
by: Carlos Andrés Rodríguez-Salazar
Published: (2021-04-01)

Interplay between Podoplanin, CD44s and CD44v in Squamous Carcinoma Cells
by: Lucía Montero-Montero, et al.
Published: (2020-09-01)