Oral dysbiosis induced by Porphyromonas gingivalis is strain-dependent in mice
Background: Porphyromonas gingivalis strain W83, one of the most widely investigated, is considered virulent in the context of periodontitis. The recently isolated P. gingivalis TDC60 has been reported to be highly pathogenic, although it has not yet been investigated in a mouse periodontitis model...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2020.1832837 |
| _version_ | 1818827254352838656 |
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| author | Emile Boyer Patricia Leroyer Ludivine Malherbe Shao Bing Fong Olivier Loréal Martine Bonnaure Mallet Vincent Meuric |
| author_facet | Emile Boyer Patricia Leroyer Ludivine Malherbe Shao Bing Fong Olivier Loréal Martine Bonnaure Mallet Vincent Meuric |
| author_sort | Emile Boyer |
| collection | DOAJ |
| description | Background: Porphyromonas gingivalis strain W83, one of the most widely investigated, is considered virulent in the context of periodontitis. The recently isolated P. gingivalis TDC60 has been reported to be highly pathogenic, although it has not yet been investigated in a mouse periodontitis model by oral gavage. Aim: Our aim was to compare the virulence of both strains by evaluating their impact on alveolar bone loss and the composition of oral microbiota. Methods: We inoculated by oral gavage C57BL/6 mice with either one of the two P. gingivalis strains and compared to a sham-treated group, without antibiotics pre-treatment. The mandibular alveolar bone of treated mice and controls were assessed, one month after the final inoculation, by microCT measurements. Moreover, at this time, we characterized their oral microbiota by 16S rRNA gene sequencing. Results: While P. gingivalis W83 successfully initiated periodontitis, TDC60-treated mice only experienced moderate lesions. Furthermore, only W83-treated mice exhibited a specific distinct microbiota, with significantly lower richness and evenness than other samples, and decreased proportions of taxa usually found in healthy individuals. Conclusion: This association between alveolar bone loss and a major persistent shift of the oral microbiota gives insights into virulence discrepancies among these bacterial strains. |
| first_indexed | 2024-12-19T00:40:38Z |
| format | Article |
| id | doaj.art-73967c9eb8334941a262bca615e4c0b2 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-19T00:40:38Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-73967c9eb8334941a262bca615e4c0b22022-12-21T20:44:35ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972020-01-0112110.1080/20002297.2020.18328371832837Oral dysbiosis induced by Porphyromonas gingivalis is strain-dependent in miceEmile Boyer0Patricia Leroyer1Ludivine Malherbe2Shao Bing Fong3Olivier Loréal4Martine Bonnaure Mallet5Vincent Meuric6Institut NUMECAN (Nutrition Metabolisms and Cancer)Institut NUMECAN (Nutrition Metabolisms and Cancer)CNRS, GMGM UMR 7156Institut NUMECAN (Nutrition Metabolisms and Cancer)Institut NUMECAN (Nutrition Metabolisms and Cancer)Institut NUMECAN (Nutrition Metabolisms and Cancer)Institut NUMECAN (Nutrition Metabolisms and Cancer)Background: Porphyromonas gingivalis strain W83, one of the most widely investigated, is considered virulent in the context of periodontitis. The recently isolated P. gingivalis TDC60 has been reported to be highly pathogenic, although it has not yet been investigated in a mouse periodontitis model by oral gavage. Aim: Our aim was to compare the virulence of both strains by evaluating their impact on alveolar bone loss and the composition of oral microbiota. Methods: We inoculated by oral gavage C57BL/6 mice with either one of the two P. gingivalis strains and compared to a sham-treated group, without antibiotics pre-treatment. The mandibular alveolar bone of treated mice and controls were assessed, one month after the final inoculation, by microCT measurements. Moreover, at this time, we characterized their oral microbiota by 16S rRNA gene sequencing. Results: While P. gingivalis W83 successfully initiated periodontitis, TDC60-treated mice only experienced moderate lesions. Furthermore, only W83-treated mice exhibited a specific distinct microbiota, with significantly lower richness and evenness than other samples, and decreased proportions of taxa usually found in healthy individuals. Conclusion: This association between alveolar bone loss and a major persistent shift of the oral microbiota gives insights into virulence discrepancies among these bacterial strains.http://dx.doi.org/10.1080/20002297.2020.1832837periodontitisanimal modelmiceporphyromonas gingivalis w83p. gingivalis tdc60oral microbiotaalveolar bone loss |
| spellingShingle | Emile Boyer Patricia Leroyer Ludivine Malherbe Shao Bing Fong Olivier Loréal Martine Bonnaure Mallet Vincent Meuric Oral dysbiosis induced by Porphyromonas gingivalis is strain-dependent in mice Journal of Oral Microbiology periodontitis animal model mice porphyromonas gingivalis w83 p. gingivalis tdc60 oral microbiota alveolar bone loss |
| title | Oral dysbiosis induced by Porphyromonas gingivalis is strain-dependent in mice |
| title_full | Oral dysbiosis induced by Porphyromonas gingivalis is strain-dependent in mice |
| title_fullStr | Oral dysbiosis induced by Porphyromonas gingivalis is strain-dependent in mice |
| title_full_unstemmed | Oral dysbiosis induced by Porphyromonas gingivalis is strain-dependent in mice |
| title_short | Oral dysbiosis induced by Porphyromonas gingivalis is strain-dependent in mice |
| title_sort | oral dysbiosis induced by porphyromonas gingivalis is strain dependent in mice |
| topic | periodontitis animal model mice porphyromonas gingivalis w83 p. gingivalis tdc60 oral microbiota alveolar bone loss |
| url | http://dx.doi.org/10.1080/20002297.2020.1832837 |
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