A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods
This study compared gradient gel electrophoresis (GGE) and light-scattering (LS) methods of determining low density lipoprotein (LDL) particle size. LDL was isolated from 27 fasting subjects. Peak particle size was determined by GGE on 3–13% gradient gels (Gradipore, Sydney, Australia) and by LS usi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1998-10-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520325074 |
| _version_ | 1818961193176399872 |
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| author | D. O'Neal P. Harrip G. Dragicevic D. Rae J.D. Best |
| author_facet | D. O'Neal P. Harrip G. Dragicevic D. Rae J.D. Best |
| author_sort | D. O'Neal |
| collection | DOAJ |
| description | This study compared gradient gel electrophoresis (GGE) and light-scattering (LS) methods of determining low density lipoprotein (LDL) particle size. LDL was isolated from 27 fasting subjects. Peak particle size was determined by GGE on 3–13% gradient gels (Gradipore, Sydney, Australia) and by LS using a Zetasizer 3000 (Malvern Instruments, Malvern, UK). Repeated measurements on a single specimen indicated a coefficient of variation (CV) of 0.3%. A correlation was noted (P < 0.0001; r = 0.78) when comparing LDL particle size determined by LS methodology and GGE. Particle diameter results obtained by LS were smaller than those obtained by GGE (23.1 ± 0.1 vs. 26.1 ± 0.1 nm; P < 0.0001). LDL particle size determined by LS methodology correlated inversely with the log of triglyceride level (P < 0.0001; r = −0.77) and positively with high density lipoprotein (HDL) cholesterol level (P < 0.002; r = 0.57).—O'Neal, D., P. Harrip, G. Dragicevic, D. Rae, and J. D. Best. A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods. J. Lipid Res. 1998. 39: 2086–2090. |
| first_indexed | 2024-12-20T12:09:32Z |
| format | Article |
| id | doaj.art-73987a692ad342b4b3f13bb75d6bfa6c |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T12:09:32Z |
| publishDate | 1998-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-73987a692ad342b4b3f13bb75d6bfa6c2022-12-21T19:41:16ZengElsevierJournal of Lipid Research0022-22751998-10-01391020862090A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methodsD. O'Neal0P. Harrip1G. Dragicevic2D. Rae3J.D. Best4Department of Medicine, University of Melbourne, St. Vincent's Hospital, Fitzroy, Victoria, Australia, 3065ATA Scientific, Williamstown, Victoria, Australia, 3016Department of Medicine, University of Melbourne, St. Vincent's Hospital, Fitzroy, Victoria, Australia, 3065Department of Chemical Pathology, St. Vincent's Hospital, Fitzroy, Victoria, Australia, 3065To whom correspondence should be addressed.; Department of Medicine, University of Melbourne, St. Vincent's Hospital, Fitzroy, Victoria, Australia, 3065; To whom correspondence should be addressed.This study compared gradient gel electrophoresis (GGE) and light-scattering (LS) methods of determining low density lipoprotein (LDL) particle size. LDL was isolated from 27 fasting subjects. Peak particle size was determined by GGE on 3–13% gradient gels (Gradipore, Sydney, Australia) and by LS using a Zetasizer 3000 (Malvern Instruments, Malvern, UK). Repeated measurements on a single specimen indicated a coefficient of variation (CV) of 0.3%. A correlation was noted (P < 0.0001; r = 0.78) when comparing LDL particle size determined by LS methodology and GGE. Particle diameter results obtained by LS were smaller than those obtained by GGE (23.1 ± 0.1 vs. 26.1 ± 0.1 nm; P < 0.0001). LDL particle size determined by LS methodology correlated inversely with the log of triglyceride level (P < 0.0001; r = −0.77) and positively with high density lipoprotein (HDL) cholesterol level (P < 0.002; r = 0.57).—O'Neal, D., P. Harrip, G. Dragicevic, D. Rae, and J. D. Best. A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods. J. Lipid Res. 1998. 39: 2086–2090.http://www.sciencedirect.com/science/article/pii/S0022227520325074LDL sizelight scatteringphoton correlation spectroscopygradient gel electrophoresis |
| spellingShingle | D. O'Neal P. Harrip G. Dragicevic D. Rae J.D. Best A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods Journal of Lipid Research LDL size light scattering photon correlation spectroscopy gradient gel electrophoresis |
| title | A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods |
| title_full | A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods |
| title_fullStr | A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods |
| title_full_unstemmed | A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods |
| title_short | A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods |
| title_sort | comparison of ldl size determination using gradient gel electrophoresis and light scattering methods |
| topic | LDL size light scattering photon correlation spectroscopy gradient gel electrophoresis |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520325074 |
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