Quantitative Mass Spectrometric Analysis and Post-Extraction Stability Assessment of the Euglenoid Toxin Euglenophycin
Euglenophycin is a recently discovered toxin produced by at least one species of euglenoid algae. The toxin has been responsible for several fish mortality events. To facilitate the identification and monitoring of euglenophycin in freshwater ponds, we have developed a specific mass spectrometric me...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-09-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/5/9/1587 |
| _version_ | 1811184650540810240 |
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| author | Paul V. Zimba Richard E. Triemer Peter D. Moeller Alexandra Rafalski Kevin Beauchesne Danielle B. Gutierrez |
| author_facet | Paul V. Zimba Richard E. Triemer Peter D. Moeller Alexandra Rafalski Kevin Beauchesne Danielle B. Gutierrez |
| author_sort | Paul V. Zimba |
| collection | DOAJ |
| description | Euglenophycin is a recently discovered toxin produced by at least one species of euglenoid algae. The toxin has been responsible for several fish mortality events. To facilitate the identification and monitoring of euglenophycin in freshwater ponds, we have developed a specific mass spectrometric method for the identification and quantitation of euglenophycin. The post-extraction stability of the toxin was assessed under various conditions. Euglenophycin was most stable at room temperature. At 8 °C there was a small, but statistically significant, loss in toxin after one day. These methods and knowledge of the toxin’s stability will facilitate identification of the toxin as a causative agent in fish kills and determination of the toxin’s distribution in the organs of exposed fish. |
| first_indexed | 2024-04-11T13:16:03Z |
| format | Article |
| id | doaj.art-7399498882eb4de6bc0fe29a7cfbd791 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T13:16:03Z |
| publishDate | 2013-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-7399498882eb4de6bc0fe29a7cfbd7912022-12-22T04:22:24ZengMDPI AGToxins2072-66512013-09-01591587159610.3390/toxins5091587Quantitative Mass Spectrometric Analysis and Post-Extraction Stability Assessment of the Euglenoid Toxin EuglenophycinPaul V. ZimbaRichard E. TriemerPeter D. MoellerAlexandra RafalskiKevin BeauchesneDanielle B. GutierrezEuglenophycin is a recently discovered toxin produced by at least one species of euglenoid algae. The toxin has been responsible for several fish mortality events. To facilitate the identification and monitoring of euglenophycin in freshwater ponds, we have developed a specific mass spectrometric method for the identification and quantitation of euglenophycin. The post-extraction stability of the toxin was assessed under various conditions. Euglenophycin was most stable at room temperature. At 8 °C there was a small, but statistically significant, loss in toxin after one day. These methods and knowledge of the toxin’s stability will facilitate identification of the toxin as a causative agent in fish kills and determination of the toxin’s distribution in the organs of exposed fish.http://www.mdpi.com/2072-6651/5/9/1587euglenaeuglenophycinstabilitytoxin |
| spellingShingle | Paul V. Zimba Richard E. Triemer Peter D. Moeller Alexandra Rafalski Kevin Beauchesne Danielle B. Gutierrez Quantitative Mass Spectrometric Analysis and Post-Extraction Stability Assessment of the Euglenoid Toxin Euglenophycin Toxins euglena euglenophycin stability toxin |
| title | Quantitative Mass Spectrometric Analysis and Post-Extraction Stability Assessment of the Euglenoid Toxin Euglenophycin |
| title_full | Quantitative Mass Spectrometric Analysis and Post-Extraction Stability Assessment of the Euglenoid Toxin Euglenophycin |
| title_fullStr | Quantitative Mass Spectrometric Analysis and Post-Extraction Stability Assessment of the Euglenoid Toxin Euglenophycin |
| title_full_unstemmed | Quantitative Mass Spectrometric Analysis and Post-Extraction Stability Assessment of the Euglenoid Toxin Euglenophycin |
| title_short | Quantitative Mass Spectrometric Analysis and Post-Extraction Stability Assessment of the Euglenoid Toxin Euglenophycin |
| title_sort | quantitative mass spectrometric analysis and post extraction stability assessment of the euglenoid toxin euglenophycin |
| topic | euglena euglenophycin stability toxin |
| url | http://www.mdpi.com/2072-6651/5/9/1587 |
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