The Effect of Sodium Butyrate on Radiosensitivity in Glioblastoma Cell
INTRODUCTION: Glioblastoma (GB) is a highly aggressive and malignant brain tumor. Currently there is no cure therapy for GB. Radiotherapy (RT) has an important role in the success of GB treatment. Therefore, the studies regarding with to increase the radiosensitivity of cells by applying of the comb...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Van Yuzuncu Yil University, School of Medicine
2019-10-01
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| Series: | Van Tıp Dergisi |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=vtd&un=VTD-24434 |
| _version_ | 1827611898191806464 |
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| author | Tahir Çakır Mustafa Güven Mehmet Taşpınar Farika Nur Denizler Bahar Kartal |
| author_facet | Tahir Çakır Mustafa Güven Mehmet Taşpınar Farika Nur Denizler Bahar Kartal |
| author_sort | Tahir Çakır |
| collection | DOAJ |
| description | INTRODUCTION: Glioblastoma (GB) is a highly aggressive and malignant brain tumor. Currently there is no cure therapy for GB. Radiotherapy (RT) has an important role in the success of GB treatment. Therefore, the studies regarding with to increase the radiosensitivity of cells by applying of the combine of the molecules having chemotherapeutic potential with RT gain importance. Epigenetic mechanisms play role in both RT and chemotherapy response. The aim of this study was to investigate the effect of sodium butyrate, a histone deacetylase inhibitor, onto the radiosensitivity when combined with RT in GB cells. METHODS: For this purpose, different concentrations of SB (0.5,1,2,3,4,6 and 8 mM) and 8Gy RT were administered to LN-405 cell lines alone and in combination. 3D conformal technique was used in RT application. Cell viability was determined by MTT after incubation 72h. RESULTS: It is detected that SB reduced cell viability on concentration-dependent but RT-independent. Cell viability in the 0.5, 1 and 2 mM groups of SB combination with RT decreased compared to the groups treated with SB alone 31.4%, 36.9% and 17.6%, respectively. According to the results, it is detected that SB increased radiosensitivity at 0.5,1,2,8 mM concentrations.
DISCUSSION AND CONCLUSION: This study showed that SB may increase the therapeutic effects of RT applications depending on the concentration. In addition, it is the first study to increase the radiosensitivity of SB by applying 3-dimensional conformal RT procedures in terms of the method of applying RT to the cell series in which acute radiation dose is given in a single fraction. |
| first_indexed | 2024-03-09T08:13:37Z |
| format | Article |
| id | doaj.art-739c9395526e40798098beec325161ec |
| institution | Directory Open Access Journal |
| issn | 2587-0351 |
| language | English |
| last_indexed | 2024-03-09T08:13:37Z |
| publishDate | 2019-10-01 |
| publisher | Van Yuzuncu Yil University, School of Medicine |
| record_format | Article |
| series | Van Tıp Dergisi |
| spelling | doaj.art-739c9395526e40798098beec325161ec2023-12-02T22:48:30ZengVan Yuzuncu Yil University, School of MedicineVan Tıp Dergisi2587-03512019-10-0126455055610.5505/vtd.2019.24434VTD-24434The Effect of Sodium Butyrate on Radiosensitivity in Glioblastoma CellTahir Çakır0Mustafa Güven1Mehmet Taşpınar2Farika Nur Denizler3Bahar Kartal4Van Yuzuncu Yıl University, Medical Faculty, Department of Medical Physics, VanVan Yuzuncu Yıl University, Medical Faculty, VanVan Yuzuncu Yıl University, Medical Faculty, Department of Medical Biology, VanVan Yuzuncu Yıl University, Medical Faculty, Department of Medical Biology, VanVan Yuzuncu Yıl University, Medical Faculty, Department of Medical Biology, VanINTRODUCTION: Glioblastoma (GB) is a highly aggressive and malignant brain tumor. Currently there is no cure therapy for GB. Radiotherapy (RT) has an important role in the success of GB treatment. Therefore, the studies regarding with to increase the radiosensitivity of cells by applying of the combine of the molecules having chemotherapeutic potential with RT gain importance. Epigenetic mechanisms play role in both RT and chemotherapy response. The aim of this study was to investigate the effect of sodium butyrate, a histone deacetylase inhibitor, onto the radiosensitivity when combined with RT in GB cells. METHODS: For this purpose, different concentrations of SB (0.5,1,2,3,4,6 and 8 mM) and 8Gy RT were administered to LN-405 cell lines alone and in combination. 3D conformal technique was used in RT application. Cell viability was determined by MTT after incubation 72h. RESULTS: It is detected that SB reduced cell viability on concentration-dependent but RT-independent. Cell viability in the 0.5, 1 and 2 mM groups of SB combination with RT decreased compared to the groups treated with SB alone 31.4%, 36.9% and 17.6%, respectively. According to the results, it is detected that SB increased radiosensitivity at 0.5,1,2,8 mM concentrations. DISCUSSION AND CONCLUSION: This study showed that SB may increase the therapeutic effects of RT applications depending on the concentration. In addition, it is the first study to increase the radiosensitivity of SB by applying 3-dimensional conformal RT procedures in terms of the method of applying RT to the cell series in which acute radiation dose is given in a single fraction.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=vtd&un=VTD-24434radiosensitivityglioblastomasodium butyrate |
| spellingShingle | Tahir Çakır Mustafa Güven Mehmet Taşpınar Farika Nur Denizler Bahar Kartal The Effect of Sodium Butyrate on Radiosensitivity in Glioblastoma Cell Van Tıp Dergisi radiosensitivity glioblastoma sodium butyrate |
| title | The Effect of Sodium Butyrate on Radiosensitivity in Glioblastoma Cell |
| title_full | The Effect of Sodium Butyrate on Radiosensitivity in Glioblastoma Cell |
| title_fullStr | The Effect of Sodium Butyrate on Radiosensitivity in Glioblastoma Cell |
| title_full_unstemmed | The Effect of Sodium Butyrate on Radiosensitivity in Glioblastoma Cell |
| title_short | The Effect of Sodium Butyrate on Radiosensitivity in Glioblastoma Cell |
| title_sort | effect of sodium butyrate on radiosensitivity in glioblastoma cell |
| topic | radiosensitivity glioblastoma sodium butyrate |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=vtd&un=VTD-24434 |
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