Pulsed-Field Ablation Using a Novel Ablation-Mapping Integrated System for Pulmonary Vein Isolation—A Preliminary Animal Study
Objective: The purpose of this study is to evaluate the preliminary safety and effect of a pulsed electric field (PEF) ablation system. Methods: The pulmonary veins (PVs) and superior vena cava (SVC) were isolated with the pulsed field ablation (PFA) system, which included a PEF generator and an ele...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Journal of Cardiovascular Development and Disease |
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| Online Access: | https://www.mdpi.com/2308-3425/9/12/425 |
| _version_ | 1797457132055953408 |
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| author | Zhihong Zhao Yonggang Chen Bin Wu Gaodong Qiu Liangjie Hong Xinhua Chen Xingwei Zhang |
| author_facet | Zhihong Zhao Yonggang Chen Bin Wu Gaodong Qiu Liangjie Hong Xinhua Chen Xingwei Zhang |
| author_sort | Zhihong Zhao |
| collection | DOAJ |
| description | Objective: The purpose of this study is to evaluate the preliminary safety and effect of a pulsed electric field (PEF) ablation system. Methods: The pulmonary veins (PVs) and superior vena cava (SVC) were isolated with the pulsed field ablation (PFA) system, which included a PEF generator and an electrode. The effects of PFA were investigated in six porcines using a novel circular catheter with combined functions (mapping/ablation) designed to work with a cardiac mapping system. The PEF generator delivered a train of biphasic pulsed electric pulses with a high amplitude (800–2000 V) and short pulse duration. The voltage mapping, PVs and SVC potentials, ostial diameters, and phrenic nerve and esophagus viability data were collected 4 weeks later, after which the animals were subsequently euthanized for gross histopathology analysis. Results: PFA 100% isolated the PVs and SVC with four applications with a mean pulse number of 100–150 pulses, causing no muscle convulsion. PFA does not cause PV stenosis or phrenic nerve dysfunction. Histological analysis confirmed 100% transmurally without any venous stenoses or phrenic injuries. Pathology follow-up showed that PFA had selectively ablated cardiomyocytes but spared blood vessels, the esophagus, and phrenic nerves; after ablation, the myocardial tissue showed homogeneous fibrosis. Conclusion: The PFA system is safe and feasible in the preliminary porcine model, which can effectively isolate PVs and SVCs. Transmural tissue damage can be achieved without phrenic palsy or stenosis. |
| first_indexed | 2024-03-09T16:16:52Z |
| format | Article |
| id | doaj.art-739d553d11d0434a94f416c2ba1e130c |
| institution | Directory Open Access Journal |
| issn | 2308-3425 |
| language | English |
| last_indexed | 2024-03-09T16:16:52Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Cardiovascular Development and Disease |
| spelling | doaj.art-739d553d11d0434a94f416c2ba1e130c2023-11-24T15:41:34ZengMDPI AGJournal of Cardiovascular Development and Disease2308-34252022-11-0191242510.3390/jcdd9120425Pulsed-Field Ablation Using a Novel Ablation-Mapping Integrated System for Pulmonary Vein Isolation—A Preliminary Animal StudyZhihong Zhao0Yonggang Chen1Bin Wu2Gaodong Qiu3Liangjie Hong4Xinhua Chen5Xingwei Zhang6Department of Cardiology, The Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, ChinaKey Laboratory of Pulsed Power Translational Medicine of Zhejiang Province, Hangzhou 311121, ChinaKey Laboratory of Pulsed Power Translational Medicine of Zhejiang Province, Hangzhou 311121, ChinaKey Laboratory of Pulsed Power Translational Medicine of Zhejiang Province, Hangzhou 311121, ChinaKey Laboratory of Pulsed Power Translational Medicine of Zhejiang Province, Hangzhou 311121, ChinaKey Laboratory of Pulsed Power Translational Medicine of Zhejiang Province, Hangzhou 311121, ChinaDepartment of Cardiology, The Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, ChinaObjective: The purpose of this study is to evaluate the preliminary safety and effect of a pulsed electric field (PEF) ablation system. Methods: The pulmonary veins (PVs) and superior vena cava (SVC) were isolated with the pulsed field ablation (PFA) system, which included a PEF generator and an electrode. The effects of PFA were investigated in six porcines using a novel circular catheter with combined functions (mapping/ablation) designed to work with a cardiac mapping system. The PEF generator delivered a train of biphasic pulsed electric pulses with a high amplitude (800–2000 V) and short pulse duration. The voltage mapping, PVs and SVC potentials, ostial diameters, and phrenic nerve and esophagus viability data were collected 4 weeks later, after which the animals were subsequently euthanized for gross histopathology analysis. Results: PFA 100% isolated the PVs and SVC with four applications with a mean pulse number of 100–150 pulses, causing no muscle convulsion. PFA does not cause PV stenosis or phrenic nerve dysfunction. Histological analysis confirmed 100% transmurally without any venous stenoses or phrenic injuries. Pathology follow-up showed that PFA had selectively ablated cardiomyocytes but spared blood vessels, the esophagus, and phrenic nerves; after ablation, the myocardial tissue showed homogeneous fibrosis. Conclusion: The PFA system is safe and feasible in the preliminary porcine model, which can effectively isolate PVs and SVCs. Transmural tissue damage can be achieved without phrenic palsy or stenosis.https://www.mdpi.com/2308-3425/9/12/425pulsed-field ablationcardiac electrophysiologypulmonary veinssuperior vena cavaarrhythmia |
| spellingShingle | Zhihong Zhao Yonggang Chen Bin Wu Gaodong Qiu Liangjie Hong Xinhua Chen Xingwei Zhang Pulsed-Field Ablation Using a Novel Ablation-Mapping Integrated System for Pulmonary Vein Isolation—A Preliminary Animal Study Journal of Cardiovascular Development and Disease pulsed-field ablation cardiac electrophysiology pulmonary veins superior vena cava arrhythmia |
| title | Pulsed-Field Ablation Using a Novel Ablation-Mapping Integrated System for Pulmonary Vein Isolation—A Preliminary Animal Study |
| title_full | Pulsed-Field Ablation Using a Novel Ablation-Mapping Integrated System for Pulmonary Vein Isolation—A Preliminary Animal Study |
| title_fullStr | Pulsed-Field Ablation Using a Novel Ablation-Mapping Integrated System for Pulmonary Vein Isolation—A Preliminary Animal Study |
| title_full_unstemmed | Pulsed-Field Ablation Using a Novel Ablation-Mapping Integrated System for Pulmonary Vein Isolation—A Preliminary Animal Study |
| title_short | Pulsed-Field Ablation Using a Novel Ablation-Mapping Integrated System for Pulmonary Vein Isolation—A Preliminary Animal Study |
| title_sort | pulsed field ablation using a novel ablation mapping integrated system for pulmonary vein isolation a preliminary animal study |
| topic | pulsed-field ablation cardiac electrophysiology pulmonary veins superior vena cava arrhythmia |
| url | https://www.mdpi.com/2308-3425/9/12/425 |
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