Both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeast
Summary Ferritin proteins have an enormous capacity to store iron in cells. In search for the best conditions to accumulate and store bioavailable iron, we made use of a double mutant null for the monothiol glutaredoxins GRX3 and GRX4. The strain grx3grx4 accumulates high iron concentrations in the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-05-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13939 |
| _version_ | 1818015879439319040 |
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| author | Nuria Pujol‐Carrion Alma Gonzalez‐Alfonso Sergi Puig Maria Angeles de laTorre‐Ruiz |
| author_facet | Nuria Pujol‐Carrion Alma Gonzalez‐Alfonso Sergi Puig Maria Angeles de laTorre‐Ruiz |
| author_sort | Nuria Pujol‐Carrion |
| collection | DOAJ |
| description | Summary Ferritin proteins have an enormous capacity to store iron in cells. In search for the best conditions to accumulate and store bioavailable iron, we made use of a double mutant null for the monothiol glutaredoxins GRX3 and GRX4. The strain grx3grx4 accumulates high iron concentrations in the cytoplasm, making the metal easily available for ferritin chelation. Here, we perform a comparative study between human (L and H) and soya bean ferritins (H1 and H2) function in the eukaryotic system Saccharomyces cerevisiae. We demonstrate that the four human and soya bean ferritin chains are successfully expressed in our model system. Upon coexpression of either both human or soya bean ferritin chains, respiratory conditions along with iron supplementation led us to obtain the maximum yields of iron stored in yeast described to date. Human and soya bean ferritin chains are functional and present equivalent properties as promoters of cell survival in iron overload conditions. The best system revealed that the four human and soya bean ferritins possess a novel function as anti‐ageing proteins in conditions of iron excess. In this respect, both ferritin chains with oxidoreductase capacity (human‐H and soya bean‐H2) bear the highest capacity to extend life suggesting the possibility of an evolutionary conservation. |
| first_indexed | 2024-04-14T07:03:43Z |
| format | Article |
| id | doaj.art-739ecc7072cf4843abedbe00daf26b65 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-04-14T07:03:43Z |
| publishDate | 2022-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-739ecc7072cf4843abedbe00daf26b652022-12-22T02:06:38ZengWileyMicrobial Biotechnology1751-79152022-05-011551525154110.1111/1751-7915.13939Both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeastNuria Pujol‐Carrion0Alma Gonzalez‐Alfonso1Sergi Puig2Maria Angeles de laTorre‐Ruiz3Cell Signalling in Yeast Unit Department of Basic Medical Sciences Institut de Recerca Biomèdica de Lleida (IRBLleida) University of Lleida Lleida 25198 SpainCell Signalling in Yeast Unit Department of Basic Medical Sciences Institut de Recerca Biomèdica de Lleida (IRBLleida) University of Lleida Lleida 25198 SpainDepartamento de Biotecnología Instituto de Agroquímica y Tecnología de Alimentos (IATA) Consejo Superior de Investigaciones Científicas (CSIC) Paterna Valencia E‐46980 SpainCell Signalling in Yeast Unit Department of Basic Medical Sciences Institut de Recerca Biomèdica de Lleida (IRBLleida) University of Lleida Lleida 25198 SpainSummary Ferritin proteins have an enormous capacity to store iron in cells. In search for the best conditions to accumulate and store bioavailable iron, we made use of a double mutant null for the monothiol glutaredoxins GRX3 and GRX4. The strain grx3grx4 accumulates high iron concentrations in the cytoplasm, making the metal easily available for ferritin chelation. Here, we perform a comparative study between human (L and H) and soya bean ferritins (H1 and H2) function in the eukaryotic system Saccharomyces cerevisiae. We demonstrate that the four human and soya bean ferritin chains are successfully expressed in our model system. Upon coexpression of either both human or soya bean ferritin chains, respiratory conditions along with iron supplementation led us to obtain the maximum yields of iron stored in yeast described to date. Human and soya bean ferritin chains are functional and present equivalent properties as promoters of cell survival in iron overload conditions. The best system revealed that the four human and soya bean ferritins possess a novel function as anti‐ageing proteins in conditions of iron excess. In this respect, both ferritin chains with oxidoreductase capacity (human‐H and soya bean‐H2) bear the highest capacity to extend life suggesting the possibility of an evolutionary conservation.https://doi.org/10.1111/1751-7915.13939 |
| spellingShingle | Nuria Pujol‐Carrion Alma Gonzalez‐Alfonso Sergi Puig Maria Angeles de laTorre‐Ruiz Both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeast Microbial Biotechnology |
| title | Both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeast |
| title_full | Both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeast |
| title_fullStr | Both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeast |
| title_full_unstemmed | Both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeast |
| title_short | Both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeast |
| title_sort | both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeast |
| url | https://doi.org/10.1111/1751-7915.13939 |
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