All-optical targeted drug delivery and real-time detection of a single cancer cell
Targeted drug delivery and real-time detection both play an important role for studying the specificity of a single cancer cell and the development of anticancer drugs. However, a method that simultaneously enables safe and efficient targeted drug delivery and noninvasive, free-label cell detection...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2019-12-01
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| Series: | Nanophotonics |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/nanoph-2019-0318 |
| _version_ | 1818689920889257984 |
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| author | Liu Xiaole Yuan Jie Wu Dong Zou Xiaobin Zheng Qing Zhang Weina Lei Hongxiang |
| author_facet | Liu Xiaole Yuan Jie Wu Dong Zou Xiaobin Zheng Qing Zhang Weina Lei Hongxiang |
| author_sort | Liu Xiaole |
| collection | DOAJ |
| description | Targeted drug delivery and real-time detection both play an important role for studying the specificity of a single cancer cell and the development of anticancer drugs. However, a method that simultaneously enables safe and efficient targeted drug delivery and noninvasive, free-label cell detection is highly desirable but challenging. Here, we report an all-optical method that combines fiber optical tweezers with laser Raman microspectroscopy, which can achieve targeted drug delivery to a single cancer cell using optical manipulation in vitro quickly and accurately by a tapered fiber probe, and simultaneously record the corresponding active characteristics of the targeted cancer cell under the contact of delivered drug through a Raman spectrometer. Using the method, drug delivery and release can be flexibly controlled by turning on/off the trapping laser beam propagating in the fiber, which can avoid the complex systems and is highly autonomous and controllable. Moreover, the detection of cell activity does not require any dye calibration and processing, and it is noninvasive. In addition, for a single suspension cell, optical trapping of the cell using another fiber tip can overcome the low efficiency of targeted drug delivery and the poor stability of the Raman spectrum caused by Brownian motion of the cell. This all-optical method provides a promising approach to conduct pharmacologic studies with the reaction of cancer cell and drugs at the level of a single cell. |
| first_indexed | 2024-12-17T12:17:47Z |
| format | Article |
| id | doaj.art-73a1693f10ca479182825ac5f65cbf2a |
| institution | Directory Open Access Journal |
| issn | 2192-8614 |
| language | English |
| last_indexed | 2024-12-17T12:17:47Z |
| publishDate | 2019-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Nanophotonics |
| spelling | doaj.art-73a1693f10ca479182825ac5f65cbf2a2022-12-21T21:49:05ZengDe GruyterNanophotonics2192-86142019-12-019361162210.1515/nanoph-2019-0318nanoph-2019-0318All-optical targeted drug delivery and real-time detection of a single cancer cellLiu Xiaole0Yuan Jie1Wu Dong2Zou Xiaobin3Zheng Qing4Zhang Weina5Lei Hongxiang6School of Materials Science and Engineering, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou 510275, ChinaSchool of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing163319, ChinaSchool of Materials Science and Engineering, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou 510275, ChinaSchool of Materials Science and Engineering, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou 510275, ChinaSchool of Materials Science and Engineering, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou 510275, ChinaSchool of Materials Science and Engineering, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou 510275, ChinaSchool of Materials Science and Engineering, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou 510275, ChinaTargeted drug delivery and real-time detection both play an important role for studying the specificity of a single cancer cell and the development of anticancer drugs. However, a method that simultaneously enables safe and efficient targeted drug delivery and noninvasive, free-label cell detection is highly desirable but challenging. Here, we report an all-optical method that combines fiber optical tweezers with laser Raman microspectroscopy, which can achieve targeted drug delivery to a single cancer cell using optical manipulation in vitro quickly and accurately by a tapered fiber probe, and simultaneously record the corresponding active characteristics of the targeted cancer cell under the contact of delivered drug through a Raman spectrometer. Using the method, drug delivery and release can be flexibly controlled by turning on/off the trapping laser beam propagating in the fiber, which can avoid the complex systems and is highly autonomous and controllable. Moreover, the detection of cell activity does not require any dye calibration and processing, and it is noninvasive. In addition, for a single suspension cell, optical trapping of the cell using another fiber tip can overcome the low efficiency of targeted drug delivery and the poor stability of the Raman spectrum caused by Brownian motion of the cell. This all-optical method provides a promising approach to conduct pharmacologic studies with the reaction of cancer cell and drugs at the level of a single cell.https://doi.org/10.1515/nanoph-2019-0318optical tweezersraman detectiondrug delivery |
| spellingShingle | Liu Xiaole Yuan Jie Wu Dong Zou Xiaobin Zheng Qing Zhang Weina Lei Hongxiang All-optical targeted drug delivery and real-time detection of a single cancer cell Nanophotonics optical tweezers raman detection drug delivery |
| title | All-optical targeted drug delivery and real-time detection of a single cancer cell |
| title_full | All-optical targeted drug delivery and real-time detection of a single cancer cell |
| title_fullStr | All-optical targeted drug delivery and real-time detection of a single cancer cell |
| title_full_unstemmed | All-optical targeted drug delivery and real-time detection of a single cancer cell |
| title_short | All-optical targeted drug delivery and real-time detection of a single cancer cell |
| title_sort | all optical targeted drug delivery and real time detection of a single cancer cell |
| topic | optical tweezers raman detection drug delivery |
| url | https://doi.org/10.1515/nanoph-2019-0318 |
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