Microglia isolation from aging mice for cell culture: A beginner’s guide
Microglia, the innate immune cell of the central nervous system, play significant roles in brain development, maintenance, homeostasis, and neuroinflammation. Although numerous methods have been developed to isolate microglia from embryonic or postnatal mouse brains, still major difficulties exist i...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2023.1082180/full |
| _version_ | 1797947959923441664 |
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| author | Akshay Kumar Vijaya Monika Iešmantaitė Virginia Mela Daiva Baltriukienė Aurelijus Burokas |
| author_facet | Akshay Kumar Vijaya Monika Iešmantaitė Virginia Mela Daiva Baltriukienė Aurelijus Burokas |
| author_sort | Akshay Kumar Vijaya |
| collection | DOAJ |
| description | Microglia, the innate immune cell of the central nervous system, play significant roles in brain development, maintenance, homeostasis, and neuroinflammation. Although numerous methods have been developed to isolate microglia from embryonic or postnatal mouse brains, still major difficulties exist in isolating microglia from adult mice, often resulting in low yield and risk of cellular activation. Therefore, there is a need for a more efficient method to isolate pure and high-yield microglia from adult mice to study various neurodegenerative diseases. The aim of this study was to develop a fully functional protocol for the isolation of microglia by comparing different protocols. We investigated the efficacy of three protocols in terms of cell yield, purity, cellular activation, cellular aging, and migration properties and proposed the modified protocol (PROTOCOL 1), which provides an optimal yield of functional microglial cells with a minimum of material and equipment and allows young researchers with little experience to isolate microglia and helps them to delve deeper into the world of neuroscience. |
| first_indexed | 2024-04-10T21:35:54Z |
| format | Article |
| id | doaj.art-73a24dadd70f4b209ac32b31d036c8a4 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-04-10T21:35:54Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-73a24dadd70f4b209ac32b31d036c8a42023-01-19T11:59:01ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022023-01-011710.3389/fncel.2023.10821801082180Microglia isolation from aging mice for cell culture: A beginner’s guideAkshay Kumar Vijaya0Monika Iešmantaitė1Virginia Mela2Daiva Baltriukienė3Aurelijus Burokas4Department of Biological Models, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius, LithuaniaDepartment of Biological Models, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius, LithuaniaDepartment of Medicine and Dermatology, Faculty of Medicine, University of Malaga, Málaga, SpainDepartment of Biological Models, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius, LithuaniaDepartment of Biological Models, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius, LithuaniaMicroglia, the innate immune cell of the central nervous system, play significant roles in brain development, maintenance, homeostasis, and neuroinflammation. Although numerous methods have been developed to isolate microglia from embryonic or postnatal mouse brains, still major difficulties exist in isolating microglia from adult mice, often resulting in low yield and risk of cellular activation. Therefore, there is a need for a more efficient method to isolate pure and high-yield microglia from adult mice to study various neurodegenerative diseases. The aim of this study was to develop a fully functional protocol for the isolation of microglia by comparing different protocols. We investigated the efficacy of three protocols in terms of cell yield, purity, cellular activation, cellular aging, and migration properties and proposed the modified protocol (PROTOCOL 1), which provides an optimal yield of functional microglial cells with a minimum of material and equipment and allows young researchers with little experience to isolate microglia and helps them to delve deeper into the world of neuroscience.https://www.frontiersin.org/articles/10.3389/fncel.2023.1082180/fullmicrogliaisolationPercollphagocytosissenescencechemotaxis |
| spellingShingle | Akshay Kumar Vijaya Monika Iešmantaitė Virginia Mela Daiva Baltriukienė Aurelijus Burokas Microglia isolation from aging mice for cell culture: A beginner’s guide Frontiers in Cellular Neuroscience microglia isolation Percoll phagocytosis senescence chemotaxis |
| title | Microglia isolation from aging mice for cell culture: A beginner’s guide |
| title_full | Microglia isolation from aging mice for cell culture: A beginner’s guide |
| title_fullStr | Microglia isolation from aging mice for cell culture: A beginner’s guide |
| title_full_unstemmed | Microglia isolation from aging mice for cell culture: A beginner’s guide |
| title_short | Microglia isolation from aging mice for cell culture: A beginner’s guide |
| title_sort | microglia isolation from aging mice for cell culture a beginner s guide |
| topic | microglia isolation Percoll phagocytosis senescence chemotaxis |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2023.1082180/full |
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