A Lamping U-Shaped Fiber Biosensor Detector for MicroRNA
This study presents a U-shaped optical fiber developed for a facile application of microRNA detection. It is fabricated by the lamping process and packaged in a quartz tube to eliminate human negligence. In addition, silanization and electrostatic self-assembly are employed to bind gold nanoparticle...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/5/1509 |
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| author | Hsin-Yi Wen Chun-Wei Huang Yu-Le Li Jing-Luen Chen Yao-Tsung Yeh Chia-Chin Chiang |
| author_facet | Hsin-Yi Wen Chun-Wei Huang Yu-Le Li Jing-Luen Chen Yao-Tsung Yeh Chia-Chin Chiang |
| author_sort | Hsin-Yi Wen |
| collection | DOAJ |
| description | This study presents a U-shaped optical fiber developed for a facile application of microRNA detection. It is fabricated by the lamping process and packaged in a quartz tube to eliminate human negligence. In addition, silanization and electrostatic self-assembly are employed to bind gold nanoparticles and miRNA-133a probe onto the silicon dioxide of the fiber surface. For Mahlavu of hepatocellular carcinoma (HCC), detection is determined by the wavelength shift and transmission loss of a U-shaped optical fiber biosensor. The spectral sensitivity of wavelength and their coefficient of determination are found at −218.319 nm/ ng/mL and 0.839, respectively. Concurrently, the sensitivity of transmission loss and their coefficient of determination are found at 162.394 dB/ ng/mL and 0.984, respectively. A method for estimating the limit of detection of Mahlavu is at 0.0133 ng/mL. The results show that the proposed U-shaped biosensor is highly specific to miRNA-133a and possesses good sensitivity to variations in specimen concentration. As such, it could be of substantial value in microRNA detection. |
| first_indexed | 2024-12-10T07:16:43Z |
| format | Article |
| id | doaj.art-73a4cd39b99841b9849ddd471fa95ddd |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-12-10T07:16:43Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-73a4cd39b99841b9849ddd471fa95ddd2022-12-22T01:57:55ZengMDPI AGSensors1424-82202020-03-01205150910.3390/s20051509s20051509A Lamping U-Shaped Fiber Biosensor Detector for MicroRNAHsin-Yi Wen0Chun-Wei Huang1Yu-Le Li2Jing-Luen Chen3Yao-Tsung Yeh4Chia-Chin Chiang5Department of Mechanical Engineering, National Kaohsiung University of Science and Technology, 415 Chien Kung Road, Kaohsiung 80778, TaiwanDepartment of Mechanical Engineering, National Kaohsiung University of Science and Technology, 415 Chien Kung Road, Kaohsiung 80778, TaiwanDepartment of Mechanical Engineering, National Kaohsiung University of Science and Technology, 415 Chien Kung Road, Kaohsiung 80778, TaiwanDepartment of Mechanical Engineering, National Kaohsiung University of Science and Technology, 415 Chien Kung Road, Kaohsiung 80778, TaiwanDepartment of Medical Laboratory Science and Biotechnology, Fooyin University, Kaohsiung 83102, TaiwanDepartment of Mechanical Engineering, National Kaohsiung University of Science and Technology, 415 Chien Kung Road, Kaohsiung 80778, TaiwanThis study presents a U-shaped optical fiber developed for a facile application of microRNA detection. It is fabricated by the lamping process and packaged in a quartz tube to eliminate human negligence. In addition, silanization and electrostatic self-assembly are employed to bind gold nanoparticles and miRNA-133a probe onto the silicon dioxide of the fiber surface. For Mahlavu of hepatocellular carcinoma (HCC), detection is determined by the wavelength shift and transmission loss of a U-shaped optical fiber biosensor. The spectral sensitivity of wavelength and their coefficient of determination are found at −218.319 nm/ ng/mL and 0.839, respectively. Concurrently, the sensitivity of transmission loss and their coefficient of determination are found at 162.394 dB/ ng/mL and 0.984, respectively. A method for estimating the limit of detection of Mahlavu is at 0.0133 ng/mL. The results show that the proposed U-shaped biosensor is highly specific to miRNA-133a and possesses good sensitivity to variations in specimen concentration. As such, it could be of substantial value in microRNA detection.https://www.mdpi.com/1424-8220/20/5/1509micrornas detectionu-shaped optical fibersilanizationbiosensor |
| spellingShingle | Hsin-Yi Wen Chun-Wei Huang Yu-Le Li Jing-Luen Chen Yao-Tsung Yeh Chia-Chin Chiang A Lamping U-Shaped Fiber Biosensor Detector for MicroRNA Sensors micrornas detection u-shaped optical fiber silanization biosensor |
| title | A Lamping U-Shaped Fiber Biosensor Detector for MicroRNA |
| title_full | A Lamping U-Shaped Fiber Biosensor Detector for MicroRNA |
| title_fullStr | A Lamping U-Shaped Fiber Biosensor Detector for MicroRNA |
| title_full_unstemmed | A Lamping U-Shaped Fiber Biosensor Detector for MicroRNA |
| title_short | A Lamping U-Shaped Fiber Biosensor Detector for MicroRNA |
| title_sort | lamping u shaped fiber biosensor detector for microrna |
| topic | micrornas detection u-shaped optical fiber silanization biosensor |
| url | https://www.mdpi.com/1424-8220/20/5/1509 |
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