Immune Infiltration, Cancer Stemness, and Targeted Therapy in Gastrointestinal Stromal Tumor
ObjectiveTo investigate the characteristics of the tumor immune microenvironment in patients with gastrointestinal stromal tumor (GIST) and identify cancer stem-like properties of GIST to screen potential druggable molecular targets.MethodsThe gene expression data of 60 patients with GIST was retrie...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.691713/full |
| _version_ | 1818990481249402880 |
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| author | Jingjing Wang Hui Ren Wenhui Wu Qianlin Zeng Jingyao Chen Juanjuan Han Minquan Lin Changhua Zhang Yulong He Mingzhe Li |
| author_facet | Jingjing Wang Hui Ren Wenhui Wu Qianlin Zeng Jingyao Chen Juanjuan Han Minquan Lin Changhua Zhang Yulong He Mingzhe Li |
| author_sort | Jingjing Wang |
| collection | DOAJ |
| description | ObjectiveTo investigate the characteristics of the tumor immune microenvironment in patients with gastrointestinal stromal tumor (GIST) and identify cancer stem-like properties of GIST to screen potential druggable molecular targets.MethodsThe gene expression data of 60 patients with GIST was retrieved from the Array Express database. CIBERSORT was applied to calculate the level of immune infiltration. ssGSEA and ESTIMATE were used to calculate the cancer stemness index and tissue purity. The Connectivity Map (CMAP) database was implemented to screen targeted drugs based on cancer stem-like properties of GIST.ResultThere was a difference in the level of immune infiltration between the metastasis and non-metastasis GIST groups. The low level of T-cell infiltration was correlated with high tumor purity and tumor stemness index, and the correlation coefficients were -0.87 and -0.61 (p < 0.001), respectively. Furthermore, there was a positive correlation between cancer stemness index and cell purity (p < 0.001). The cancer stemness index in the metastasis group was higher than that in the non-metastasis group (p = 0.0017). After adjusting for tumor purity, there was no significant correlation between T-cell infiltration and cancer stemness index (p = 0.086). Through the pharmacological mechanism of topoisomerase inhibitors, six molecular complexes may be the targets of GIST treatment.ConclusionImmune infiltration in GIST patients is related to cancer stem-like properties, and the correlation relies on tumor purity. Cancer stemness index can be used as a new predictive biomarker of tumor metastasis and targets of drug therapy for GIST patients. |
| first_indexed | 2024-12-20T19:55:03Z |
| format | Article |
| id | doaj.art-73a7be628fcc4467a38e004de0bebabd |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-20T19:55:03Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-73a7be628fcc4467a38e004de0bebabd2022-12-21T19:28:11ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-12-011210.3389/fimmu.2021.691713691713Immune Infiltration, Cancer Stemness, and Targeted Therapy in Gastrointestinal Stromal TumorJingjing Wang0Hui Ren1Wenhui Wu2Qianlin Zeng3Jingyao Chen4Juanjuan Han5Minquan Lin6Changhua Zhang7Yulong He8Mingzhe Li9Department of Laboratory, Hexian Memorial Hospital of Panyu District, Guangzhou, ChinaDigestive Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDigestive Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDigestive Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDigestive Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDepartment of Laboratory, Hexian Memorial Hospital of Panyu District, Guangzhou, ChinaDepartment of Laboratory, Hexian Memorial Hospital of Panyu District, Guangzhou, ChinaDigestive Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDigestive Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDigestive Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaObjectiveTo investigate the characteristics of the tumor immune microenvironment in patients with gastrointestinal stromal tumor (GIST) and identify cancer stem-like properties of GIST to screen potential druggable molecular targets.MethodsThe gene expression data of 60 patients with GIST was retrieved from the Array Express database. CIBERSORT was applied to calculate the level of immune infiltration. ssGSEA and ESTIMATE were used to calculate the cancer stemness index and tissue purity. The Connectivity Map (CMAP) database was implemented to screen targeted drugs based on cancer stem-like properties of GIST.ResultThere was a difference in the level of immune infiltration between the metastasis and non-metastasis GIST groups. The low level of T-cell infiltration was correlated with high tumor purity and tumor stemness index, and the correlation coefficients were -0.87 and -0.61 (p < 0.001), respectively. Furthermore, there was a positive correlation between cancer stemness index and cell purity (p < 0.001). The cancer stemness index in the metastasis group was higher than that in the non-metastasis group (p = 0.0017). After adjusting for tumor purity, there was no significant correlation between T-cell infiltration and cancer stemness index (p = 0.086). Through the pharmacological mechanism of topoisomerase inhibitors, six molecular complexes may be the targets of GIST treatment.ConclusionImmune infiltration in GIST patients is related to cancer stem-like properties, and the correlation relies on tumor purity. Cancer stemness index can be used as a new predictive biomarker of tumor metastasis and targets of drug therapy for GIST patients.https://www.frontiersin.org/articles/10.3389/fimmu.2021.691713/fullgastrointestinal stromal tumortargeted therapyimmune infiltrationcancer stem cellstumor immune microenvironment |
| spellingShingle | Jingjing Wang Hui Ren Wenhui Wu Qianlin Zeng Jingyao Chen Juanjuan Han Minquan Lin Changhua Zhang Yulong He Mingzhe Li Immune Infiltration, Cancer Stemness, and Targeted Therapy in Gastrointestinal Stromal Tumor Frontiers in Immunology gastrointestinal stromal tumor targeted therapy immune infiltration cancer stem cells tumor immune microenvironment |
| title | Immune Infiltration, Cancer Stemness, and Targeted Therapy in Gastrointestinal Stromal Tumor |
| title_full | Immune Infiltration, Cancer Stemness, and Targeted Therapy in Gastrointestinal Stromal Tumor |
| title_fullStr | Immune Infiltration, Cancer Stemness, and Targeted Therapy in Gastrointestinal Stromal Tumor |
| title_full_unstemmed | Immune Infiltration, Cancer Stemness, and Targeted Therapy in Gastrointestinal Stromal Tumor |
| title_short | Immune Infiltration, Cancer Stemness, and Targeted Therapy in Gastrointestinal Stromal Tumor |
| title_sort | immune infiltration cancer stemness and targeted therapy in gastrointestinal stromal tumor |
| topic | gastrointestinal stromal tumor targeted therapy immune infiltration cancer stem cells tumor immune microenvironment |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.691713/full |
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