Protocol for genome editing in wild allotetraploid rice Oryza alta

Protocol for genome editing in wild allotetraploid rice Oryza alta

Summary: We present a protocol for Agrobacterium-mediated genetic transformation and genome editing in wild allotetraploid rice Oryza alta. We detail steps to induce embryogenic calli from mature seeds and co-cultivate with Agrobacterium after infection. We further describe how to select transformed...

Full description

Bibliographic Details
Main Authors:	Jingkun Zhang, Peng Zeng, Hong Yu, Xiangbing Meng, Jiayang Li
Format:	Article
Language:	English
Published:	Elsevier 2022-12-01
Series:	STAR Protocols
Subjects:	Plant sciences CRISPR Biotechnology and bioengineering
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722006694

Similar Items

An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments
by: Xiaoqian Zeng, et al.
Published: (2023-09-01)

Genome editing in Penicillium funiculosum using in vitro assembled CRISPR-Cas9 ribonucleoprotein complexes
by: Tulika Sinha, et al.
Published: (2022-09-01)

Protocol for large DNA transgenesis in mice using the Cas9+Bxb1 toolbox
by: Valerie Erhardt, et al.
Published: (2024-06-01)

Protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes
by: Fernando H. Biase, et al.
Published: (2024-03-01)

Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems
by: Zhiying Zhao, et al.
Published: (2022-09-01)

Large-scale single-cell cloning of genome-edited cultured human cells by On-chip SPiS
by: Gou Takahashi, et al.
Published: (2023-09-01)

Genome editing is induced in a binary manner in single human cells
by: Gou Takahashi, et al.
Published: (2022-12-01)

Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates
by: Noah Stanis, et al.
Published: (2023-09-01)

Current Developments in Biotechnology and Bioengineering : Functional Genomics and Metabolic Engineering /
by: Gunasekaran, Paramasamy, editors, et al.
Published: (2016)

A protocol to investigate neural coupling of brain oscillations in rodents using in vivo electrophysiological recordings
by: Cong Wang, et al.
Published: (2023-09-01)

A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry
by: Zhengyuan Pang, et al.
Published: (2022-12-01)

Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice
by: Zhen-Ping Zou, et al.
Published: (2023-06-01)

Chemical biotechnology and bioengineering /
by: Qian, Xuhong, author, et al.
Published: (2015)

Career development in bioengineering and biotechnology /
by: Madhavan, Guruprasad, et al.
Published: (2008)

Biotechnology and Bioengineering /
by: Jacob-Lopes, Eduardo, editor, et al.
Published: (2019)

Essence and Applications of Biotechnology /
by: Spencer, Valentine, author 652547, et al.
Published: (2012)

Advanced Biotechnology and its Products /
by: Lacey, Rosalia, author 644550, et al.
Published: (2012)

Biotechnology and bioengineering
Published: (1950)

Protocol for gene characterization in Aspergillus niger using 5S rRNA-CRISPR-Cas9-mediated Tet-on inducible promoter exchange
by: Xiaomei Zheng, et al.
Published: (2022-12-01)

Protocol to engineer nanofilms embedded lipid nanoparticles for controlled and targeted drug delivery (NECTAR)
by: Rashi Porwal, et al.
Published: (2023-03-01)

Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection
by: Kun Li, et al.
Published: (2023-09-01)

A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria
by: Sonal Fande, et al.
Published: (2023-06-01)

Protocol to access unknown flanking DNA sequences using Wristwatch-PCR for genome-walking
by: Lingqin Wang, et al.
Published: (2023-03-01)

Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses
by: Yoshitaka J. Sei, et al.
Published: (2023-12-01)

Protocol for near-infrared optogenetics manipulation of neurons and motor behavior in C. elegans using emissive upconversion nanoparticles
by: Ruipeng Wang, et al.
Published: (2024-03-01)

Protocol to retrieve unknown flanking DNA sequences using semi-site-specific PCR-based genome walking
by: Hong Chen, et al.
Published: (2024-03-01)

A stereoselective protocol for the synthesis of a molecular cinquefoil (51) knot
by: Jing Han, et al.
Published: (2023-09-01)

History and trends in bioprocessing and biotransformation /
by: Scheper, T., et al.
Published: (2002)

Handbook of Biotechnology and Biological Engineering /
by: Lacey, Rosalia, author 644550, et al.
Published: (2012)

Biology for engineers /
by: Johnson, Arthur T. (Arthur Thomas), 1941-
Published: (2011)

Integration of in vivo electrophysiology and optogenetics in rodents with PEDOT:PSS neural electrode array
by: Young Uk Cho, et al.
Published: (2024-03-01)

Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models
by: Adele Moatti, et al.
Published: (2023-06-01)

Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells
by: Lorena Mazuecos, et al.
Published: (2023-09-01)

Fabricating self-powered microfluidic devices via 3D printing for manipulating fluid flow
by: Sung Oh Woo, et al.
Published: (2022-06-01)

Biotechnology and bioengineering /
by: Flynne, William G.
Published: (2008)

Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains
by: Hannah Tabakh, et al.
Published: (2023-03-01)

Protocol to assess extravasation of fluorescent molecules in mice after ultrasound-mediated blood-brain barrier opening
by: Lea Peko, et al.
Published: (2024-03-01)

Protocol for designing and preparing gallium particles using cell membrane encapsulation for applications in melanoma cryoablation therapy
by: Xuelin Wang, et al.
Published: (2022-12-01)

Protocol for a placenta-on-a-chip model using trophoblasts differentiated from human induced pluripotent stem cells
by: Agathe Lermant, et al.
Published: (2024-03-01)

Protocol for the operation of a breathing and vaping biomimetic robot to delineate real-time inhaled particle profile of electronic cigarettes
by: Rachel F. Bogdanoff, et al.
Published: (2022-12-01)