Evaluation of the synergistic effect of ceftaroline against methicillin-resistant Staphylococcus aureus
Objectives: We aimed to determine the synergistic effects of ceftaroline (CPT) in combination with daptomycin (DAP), vancomycin (VAN), or linezolid (LNZ) against various methicillin-resistant Staphylococcus aureus (MRSA) strains. Methods: MRSA strains randomly selected from 2014 to 2018 were studied...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | International Journal of Infectious Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971222003186 |
| _version_ | 1817995050167042048 |
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| author | Cheng-En Tsai Chia-Jui Yang Yu-Chung Chuang Jann-Tay Wang Wang-Huei Sheng Yee-Chun Chen Shan-Chwen Chang |
| author_facet | Cheng-En Tsai Chia-Jui Yang Yu-Chung Chuang Jann-Tay Wang Wang-Huei Sheng Yee-Chun Chen Shan-Chwen Chang |
| author_sort | Cheng-En Tsai |
| collection | DOAJ |
| description | Objectives: We aimed to determine the synergistic effects of ceftaroline (CPT) in combination with daptomycin (DAP), vancomycin (VAN), or linezolid (LNZ) against various methicillin-resistant Staphylococcus aureus (MRSA) strains. Methods: MRSA strains randomly selected from 2014 to 2018 were studied. Checkerboard titration and in vitro time-kill analyses were used to determine the synergistic activities of the antibiotic combinations. Results: A total of 10 genetically distinct MRSA strains were included in this study. The checkerboard titration analysis revealed that the CPT-DAP, CPT-VAN, and CPT-LNZ combinations had a synergistic effect against 30%, 10%, and 10% of the selected MRSA strains, respectively. Using time-kill analysis, we showed that CPT-DAP exhibited a significant synergistic and sustained bactericidal effect against both DAP-susceptible (Δ colony-forming units/ml, –5.79; P = 0.0495) and DAP-resistant (Δ colony-forming units/ml, –6.40; P = 0.0463) MRSA strains at a concentration of 0.5 × the minimum inhibitory concentration of CPT plus 0.5 × the minimum inhibitory concentration of DAP. No synergistic bactericidal effects were observed for the CPT-VAN and CPT-LNZ combinations against the selected strains. Conclusion: The CPT-DAP combination showed better synergistic activity than the CPT-VAN and CPT-LNZ combinations against the enrolled MRSA strains. DAP, rather than VAN or LNZ, might be a better choice for CPT combination in the treatment of MRSA infections. |
| first_indexed | 2024-04-14T02:01:35Z |
| format | Article |
| id | doaj.art-73aa52a48b8a4700a184b6c9b44fc0eb |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-04-14T02:01:35Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-73aa52a48b8a4700a184b6c9b44fc0eb2022-12-22T02:18:50ZengElsevierInternational Journal of Infectious Diseases1201-97122022-09-01122230236Evaluation of the synergistic effect of ceftaroline against methicillin-resistant Staphylococcus aureusCheng-En Tsai0Chia-Jui Yang1Yu-Chung Chuang2Jann-Tay Wang3Wang-Huei Sheng4Yee-Chun Chen5Shan-Chwen Chang6School of Medicine, National Taiwan University College of Medicine, Taipei City, TaiwanDepartment of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City, Taiwan; School of Medicine, National Yang Ming Chiao Tung University, Taipei, TaiwanDepartment of Internal Medicine, National Taiwan University Hospital, Taipei City, Taiwan; Correspondence: Dr. Yu-Chung Chuang, Department of Internal Medicine, National Taiwan University Hospital, 7 Chung-Shan South Road, Taipei, Taiwan, 100, Telephone: 886-2-23123456 ext. 63015; Fax: 886-2-23971412.Department of Internal Medicine, National Taiwan University Hospital, Taipei City, TaiwanDepartment of Internal Medicine, National Taiwan University Hospital, Taipei City, TaiwanDepartment of Internal Medicine, National Taiwan University Hospital, Taipei City, TaiwanDepartment of Internal Medicine, National Taiwan University Hospital, Taipei City, TaiwanObjectives: We aimed to determine the synergistic effects of ceftaroline (CPT) in combination with daptomycin (DAP), vancomycin (VAN), or linezolid (LNZ) against various methicillin-resistant Staphylococcus aureus (MRSA) strains. Methods: MRSA strains randomly selected from 2014 to 2018 were studied. Checkerboard titration and in vitro time-kill analyses were used to determine the synergistic activities of the antibiotic combinations. Results: A total of 10 genetically distinct MRSA strains were included in this study. The checkerboard titration analysis revealed that the CPT-DAP, CPT-VAN, and CPT-LNZ combinations had a synergistic effect against 30%, 10%, and 10% of the selected MRSA strains, respectively. Using time-kill analysis, we showed that CPT-DAP exhibited a significant synergistic and sustained bactericidal effect against both DAP-susceptible (Δ colony-forming units/ml, –5.79; P = 0.0495) and DAP-resistant (Δ colony-forming units/ml, –6.40; P = 0.0463) MRSA strains at a concentration of 0.5 × the minimum inhibitory concentration of CPT plus 0.5 × the minimum inhibitory concentration of DAP. No synergistic bactericidal effects were observed for the CPT-VAN and CPT-LNZ combinations against the selected strains. Conclusion: The CPT-DAP combination showed better synergistic activity than the CPT-VAN and CPT-LNZ combinations against the enrolled MRSA strains. DAP, rather than VAN or LNZ, might be a better choice for CPT combination in the treatment of MRSA infections.http://www.sciencedirect.com/science/article/pii/S1201971222003186Methicillin-resistant Staphylococcus aureusCeftarolineSynergyDaptomycinLinezolidVancomycin |
| spellingShingle | Cheng-En Tsai Chia-Jui Yang Yu-Chung Chuang Jann-Tay Wang Wang-Huei Sheng Yee-Chun Chen Shan-Chwen Chang Evaluation of the synergistic effect of ceftaroline against methicillin-resistant Staphylococcus aureus International Journal of Infectious Diseases Methicillin-resistant Staphylococcus aureus Ceftaroline Synergy Daptomycin Linezolid Vancomycin |
| title | Evaluation of the synergistic effect of ceftaroline against methicillin-resistant Staphylococcus aureus |
| title_full | Evaluation of the synergistic effect of ceftaroline against methicillin-resistant Staphylococcus aureus |
| title_fullStr | Evaluation of the synergistic effect of ceftaroline against methicillin-resistant Staphylococcus aureus |
| title_full_unstemmed | Evaluation of the synergistic effect of ceftaroline against methicillin-resistant Staphylococcus aureus |
| title_short | Evaluation of the synergistic effect of ceftaroline against methicillin-resistant Staphylococcus aureus |
| title_sort | evaluation of the synergistic effect of ceftaroline against methicillin resistant staphylococcus aureus |
| topic | Methicillin-resistant Staphylococcus aureus Ceftaroline Synergy Daptomycin Linezolid Vancomycin |
| url | http://www.sciencedirect.com/science/article/pii/S1201971222003186 |
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