Effect of ciRS-7 expression on clear cell renal cell carcinoma progression
Abstract. Background. Circular RNA ciRS-7 has been reported to be involved in the progression of various cancers. However, ciRS-7 expression and its role in clear cell renal cell carcinoma (ccRCC) progression remains unclear. This study aimed to investigate the effect of ciRS-7 expression on ccRCC a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2020-09-01
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| Series: | Chinese Medical Journal |
| Online Access: | http://journals.lww.com/10.1097/CM9.0000000000000867 |
| _version_ | 1831544746994040832 |
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| author | Yan-Hui Zhao Zhi Wang Na Zhang Tao Cui Yan-Hui Zhang Yuan-Yuan Ji. |
| author_facet | Yan-Hui Zhao Zhi Wang Na Zhang Tao Cui Yan-Hui Zhang Yuan-Yuan Ji. |
| author_sort | Yan-Hui Zhao |
| collection | DOAJ |
| description | Abstract. Background. Circular RNA ciRS-7 has been reported to be involved in the progression of various cancers. However, ciRS-7 expression and its role in clear cell renal cell carcinoma (ccRCC) progression remains unclear. This study aimed to investigate the effect of ciRS-7 expression on ccRCC and the related signaling pathway.
Methods. ciRS-7 expression was analyzed using quantitative reverse transcription polymerase chain reaction in 87 pairs of ccRCC and matched adjacent normal tissues. The role of ciRS-7 in ccRCC cell proliferation and invasion was determined using the cell counting kit-8 and invasion assays, respectively. Potential mechanisms underlying the role of ciRS-7 in promoting ccRCC progression were explored by Western blotting. The relationship between the expression of ciRS-7 and features of ccRCC was analyzed by the Chi-square test and progression-free survival was determined using a Kaplan-Meier plot.
Results. ciRS-7 was overexpressed in ccRCC tissues compared with that in matched adjacent normal tissues. In addition, ciRS-7 up-regulation was closely associated with tumor diameter (P = 0.050), clinical stage (P = 0.009), and distant metastasis (P = 0.007). ciRS-7 knockdown in 786O and 769P cells markedly inhibited their proliferative and invasive abilities. In addition, ciRS-7 inhibition reduced phosphorylated epidermal growth factor receptor (p-EGFR) and phosphorylated serine/threonine kinase (p-Akt) levels.
Conclusions. ciRS-7 up-regulation could promote ccRCC cell proliferation and invasion, which may be related with the EGFR/Akt signaling pathway. ciRS-7 might be a potential ccRCC therapeutic target. |
| first_indexed | 2024-12-17T01:15:44Z |
| format | Article |
| id | doaj.art-73ac69b3aa16415a92d8e5c3bf84f200 |
| institution | Directory Open Access Journal |
| issn | 0366-6999 2542-5641 |
| language | English |
| last_indexed | 2024-12-17T01:15:44Z |
| publishDate | 2020-09-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj.art-73ac69b3aa16415a92d8e5c3bf84f2002022-12-21T22:09:01ZengWolters KluwerChinese Medical Journal0366-69992542-56412020-09-01133172084208910.1097/CM9.0000000000000867202009050-00010Effect of ciRS-7 expression on clear cell renal cell carcinoma progressionYan-Hui ZhaoZhi WangNa ZhangTao CuiYan-Hui ZhangYuan-Yuan Ji.Abstract. Background. Circular RNA ciRS-7 has been reported to be involved in the progression of various cancers. However, ciRS-7 expression and its role in clear cell renal cell carcinoma (ccRCC) progression remains unclear. This study aimed to investigate the effect of ciRS-7 expression on ccRCC and the related signaling pathway. Methods. ciRS-7 expression was analyzed using quantitative reverse transcription polymerase chain reaction in 87 pairs of ccRCC and matched adjacent normal tissues. The role of ciRS-7 in ccRCC cell proliferation and invasion was determined using the cell counting kit-8 and invasion assays, respectively. Potential mechanisms underlying the role of ciRS-7 in promoting ccRCC progression were explored by Western blotting. The relationship between the expression of ciRS-7 and features of ccRCC was analyzed by the Chi-square test and progression-free survival was determined using a Kaplan-Meier plot. Results. ciRS-7 was overexpressed in ccRCC tissues compared with that in matched adjacent normal tissues. In addition, ciRS-7 up-regulation was closely associated with tumor diameter (P = 0.050), clinical stage (P = 0.009), and distant metastasis (P = 0.007). ciRS-7 knockdown in 786O and 769P cells markedly inhibited their proliferative and invasive abilities. In addition, ciRS-7 inhibition reduced phosphorylated epidermal growth factor receptor (p-EGFR) and phosphorylated serine/threonine kinase (p-Akt) levels. Conclusions. ciRS-7 up-regulation could promote ccRCC cell proliferation and invasion, which may be related with the EGFR/Akt signaling pathway. ciRS-7 might be a potential ccRCC therapeutic target.http://journals.lww.com/10.1097/CM9.0000000000000867 |
| spellingShingle | Yan-Hui Zhao Zhi Wang Na Zhang Tao Cui Yan-Hui Zhang Yuan-Yuan Ji. Effect of ciRS-7 expression on clear cell renal cell carcinoma progression Chinese Medical Journal |
| title | Effect of ciRS-7 expression on clear cell renal cell carcinoma progression |
| title_full | Effect of ciRS-7 expression on clear cell renal cell carcinoma progression |
| title_fullStr | Effect of ciRS-7 expression on clear cell renal cell carcinoma progression |
| title_full_unstemmed | Effect of ciRS-7 expression on clear cell renal cell carcinoma progression |
| title_short | Effect of ciRS-7 expression on clear cell renal cell carcinoma progression |
| title_sort | effect of cirs 7 expression on clear cell renal cell carcinoma progression |
| url | http://journals.lww.com/10.1097/CM9.0000000000000867 |
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